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您的检索式：作者名="Ryszard Grygorczyk"
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| 1 | Heat induces adenosine triphosphate release from mast cells in vitro:a putative mechanism for moxibustion显示文摘OBJECTIVE:To investigate the role of adenosine triphosphate(ATP) purinergic signaling in mast cells(MCs) modulated by heat to further understand the molecular mechanisms of moxibustion.METHODS:Skin temperatures induced by monkshood cake moxibustion were evaluated by measuring the Neiguan acupoint(PC 6) from 31 participants with a digital thermocouple thermometer.Temperatures of 43 ℃ and 52 ℃ were applied to cultured human leukemia mast cell line HMC-1 in vitro.Calcium fluorescence was applied to detect intracellular Ca^(2+)([Ca^(2+)]_i).Extracellular ATP contents were measured by luciferin-luciferase assay.RESULTS:Maximum skin temperatures mostly ranged from 40-45 ℃,but some reached up to50 ℃.Both 43 ℃,and 52 ℃ induced MC degranulation,which was accompanied by an increase in[Ca^(2+)]_i and ATP release.Complexing extracellular Ca^(2+) with 5 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid(EGTA) inhibited the noxious heat-induced elevation of[Ca^(2+)]_i and prevented the enhanced ATP secretion by those cells at 52℃ but not 43 ℃.CONCLUSION:Monkshood cake moxibustion can generate heat sufficient to trigger cellular events of MCs,including degranulation,[Ca^(2+)]_i elevation,and ATP release,suggesting that purinergic signals originating from MCs are possibly the initiating response of acupoints to moxibustion. | Hu Lei Wang Lina Wei Jianzi Ryszard Grygorczyk Shen Xueyong Wolfgang Schwarz | 2015 | Journal of Traditional Chinese Medicine2015，35，3： | 12 |
| 2 | Volume-sensitive release of organic osmolytes in the humanlung epithelial cell line A549: role of the 5-lipoxygenase 显示文摘 | Jacob Bak Holm Ryszard Grygorczyk Ian Henry Lambert | 2013 | Am J Physiol Cell Physiol2013，305，1： | 1 |
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