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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Ethanol impaired neuronal migration is associated with reduced aspartyl-asparaginylbeta-hydroxylase expression显示文摘 | Carter J J Tong M Silbermann E Lahousse S A Ding F F Longato L Roper N Wands J R | | 0，，03： | 1 |
| 3 | Hole doping of the CuO2 chains(La,Sr,Ca)14Cu24 O41显示文摘 | CARTER S A BATLOGG B CAVA R J KRAJEWSKI J J PECK JR W F RICE T M | 1996 | Phys Rev Lett1996，77，7： | 1 |
| 4 | Evidence for recombination in the flagellin locus of Campylobacter jejuni : implications for the flagellin gene typing scheme显示文摘 | HARRINGTON C S THOMSON-CARTER F M CARTER P E | 1997 | J Clin Microbiol1997，35，： | 1 |
| 5 | Effect of alternative pathway therapy on branched chain amino acid metabolism in urea cycle dis- order patients显示文摘 | Scaglia F Carter S O'Brine WE | 2004 | Mol Genet Metab2004，8110，： | 1 |
| 6 | Limit state solutions based on linear elastic solutions with spatially varying elastic modulus 显示文摘 | PONTER A R S CARTER K F | 1997 | Computer Methods in Applied Mechanics and Engineering1997，140，34： | 1 |
| 7 | Factors Influencing the Formation of Pinholes in Gray and Ductile Iron 显示文摘 | S F Carter W J Evans J C Harkness | 1979 | AFS Transactions1979，87，： | 1 |
| 8 | Results after surgical and en- dovascular treatment of paraclinoid aneurysms by a combined neurovas- cular team显示文摘 | Hob B L Carter B S Budzik R F | 2001 | Neurosurgery2001，48，1： | 1 |
| 9 | Protein synthesis, nitrogen excretion and long-term growth of juvenile Pleuronectesflesus 显示文摘 | Carter C G Houlihan D F Owen S F | 1998 | J Fish Biol1998，53，： | 1 |
| 10 | Physical properties of constructed and undisturbed soils显示文摘 | Potter K N Carter F S Doll E C | 1988 | Soil Science Society o{ America Journal1988，52，5： | 1 |
| 11 | Ganeden- BC30TM cell wall and metabolites: anti-inflammatory and immune modulating effects in vitro显示文摘 | Jensen G S Benson K F Carter S G | 2010 | BMC Immunology2010，11，： | 1 |
| 12 | Noninvasive assessment of pancreatic beta-cell function in vivo with manganese-enhanced magnetic resonance imaging 显示文摘 | ANTKOWIAK P F TERSEY S A CARTER J D | 2009 | Am J Physiol Endocrinol Metab2009，296，3： | 1 |
| 13 | Community dermatology 显示文摘 | Alian S Crowley N Carter F | 1993 | BMJ1993，306，6877： | 1 |
| 14 | Physical properties of constructed and unconstructed soils显示文摘 | Potter K N Carter F S Doll E C | 1988 | Soil Sci Soc Am J1988，52，： | 1 |
| 15 | Self-fertility in Epilobium obcordatum (Onagraceae)显示文摘 | Seavey S F Carter S K | 1993 | American Journal of Botany1993，81，： | 1 |
| 16 | Physical properties of constructed and unconstructed soils显示文摘 | Potter K N Carter F S Doll E C | 1988 | Soil Science Society of America Journal1988，52，5： | 1 |
| 17 | Evidence forrecombination in the flagellin locus of Campylobacter jejuni ;implications for the flagellin gene typing scheme 显示文摘 | Harrington C S Thomson-Carter F M Carter P E | 1997 | J ClinMcrobiol1997，35，9： | 1 |
| 18 | Physical properties of constructed and unconstructed soils显示文摘 | Potter K N Carter F S Doll E C | 1988 | Soil Science Society of America Journal1988，52，： | 1 |
| 19 | International students' motivations for studying in UK HE, insights into the choice and decision making of African students显示文摘 | MARINGE F CARTER S | 2007 | International Journal of Educational Marketing2007，21，6： | 1 |
| 20 | The vineland adaptive behavior scales:supplementary norms for individu- als with autism 显示文摘 | Carter A S Volkmar F R Sparrow S S | 1998 | Journal of Autism and Developmental Disorders1998，，28： | 1 |
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