|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Smoothelin,a new marker to determine the origin of liver fibrogenic cells显示文摘AIM:To explore this hypothesis that smooth muscle cells may be capable of acquiring a myofibroblastic phenotype,we have studied the expression of smoothelin in fibrotic conditions.METHODS:Normal liver tissue(n=3)was obtained from macroscopically normal parts of hepatectomy,taken at a distance from hemangiomas.Pathological specimens included post-burn cutaneous hypertrophic scars(n=3),fibrotic liver tissue(n=5),cirrhotic tissue(viral and alcoholic hepatitis)(n=5),and hepatocellular carcinomas(n=5).Tissue samples were fixed in 10%formalin and embedded in paraffin for immunohistochemistry or were immediately frozen in liquid nitrogen-cooled isopentane for confocal microscopy analysis.Sections were stained with antibodies against smoothelin,which is expressed exclusively by smooth muscle cells,andα-smooth muscle actin,which is expressed by both smooth muscle cells and myofibroblasts.RESULTS:In hypertrophic scars,α-smooth muscle actin was detected in vascular smooth muscle cells and in numerous myofibroblasts present in and around nodules,whereas smoothelin was exclusively expressed in vascular smooth muscle cells.In the normal liver,vascular smooth muscle cells were the only cells that expressα-smooth muscle actin and smoothelin.In fibrotic areas of the liver,myofibroblasts expressingα-smooth muscle actin were detected.Myofibroblasts co-expressingα-smooth muscle actin and smoothelin were observed,and their number was slightly increased in parallel with the degree of fibrosis(absent in liver with mild or moderate fibrosis;5%to 10%positive in liver showing severe fibrosis).In cirrhotic septa,numerous myofibroblasts co-expressedα-smooth muscle actin and smoothelin(more than 50%).In hepatocellular carcinomas,the same pattern of expression forα-smooth muscle actin and smoothelin was observed in the stroma reaction surrounding the tumor and around tumoral cell plates.In all pathological liver samples,α-smooth muscle actin and smoothelin were co-expressed in vascular smooth muscle cells.CONCLUSION:During development of advanced liver fibrosis,a subpopulation of myofibroblasts expressing smoothelin may be derived from vascular smooth muscle cells,illustrating the different cellular origins of myofibroblasts. | Sébastien Lepreux Christelle Guyot Fabrice Billet Chantal Combe Charles Balabaud Paulette Bioulac-Sage Alexis Desmoulière | 2013 | World Journal of Gastroenterology2013，19，48： | 2 |
| 2 | LC/ES1-MS/MS character- isation of procyanidins and propelargonidins responsible for the strong antioxidant activity of the edible halophyte Mesembryanthemum edule L显示文摘 | FALLEH H OUESLATI S GUYOT S | 2011 | Food Chem2011，127，： | 1 |
| 3 | Chemopreventive properties of apple procyanidins on human colon cancer- derived metastatic SW620 cells and in a rat model of co- lon carcinogenesis 显示文摘 | GOSSI~ F GUYOT S ROUSSI S | 2005 | Carcinogenesis2005，26，7： | 1 |
| 4 | In vitro selection and charac- terization of resistance to macrolides and related antibiotics in Myco- plasma pneumoniae 显示文摘 | Pereyre S Guyot C Renaudin H | 2004 | Antimicrob Agents Chemother2004，48，2： | 1 |
| 5 | Comparative responses to sodium selenite and organic selenium supplements in Belgian Blue cows and calves显示文摘 | Guyot H Spring P Andrieu S | 2007 | Livest Sci2007，111，3： | 1 |
| 6 | Hepatic fibrosis and cirrhosis: The (myo) fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | The International Journal of Biochemistry Cell Biology2006，38，1： | 1 |
| 7 | Hepatic fibrosis and cirrho-sis:the(myo)fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combe C | 2006 | Int JBiochem Cell Biol2006，38，2： | 1 |
| 8 | In vitro selection and charac- terization of resistance to macrolides and related antibiotics in Mycoplasma pneumortiae显示文摘 | Pereyre S Guyot C Renaudin H | 2004 | Antimicrob Agents Chemother2004，48，2： | 1 |
| 9 | Texture analysis based on local analysis of the Bidimensional Empirical Mode Decomposition显示文摘 | Nunes J C Guyot S Delechelle E | 2005 | Machine Vision and Applications2005，16，： | 1 |
| 10 | Texture Anal- ysis Based on Local Analysis of the Bidimensional Empirical Mode Decomposition显示文摘 | Nunes J C Guyot S Del'Eehelle E | 2005 | Machine Vision and Applications2005，16，： | 1 |
| 11 | Diagnosis of the follicular variant of papillary thyroid carcinoma Significance of immunohistochemistry 显示文摘 | GuyOtant S Michalak S Vain I | 2003 | Ann Pathol2003，23，1： | 1 |
| 12 | Polyphenol profiles of French cider apple varieties显示文摘 | SANONER P GUYOT S | 1999 | J Agric Food Chem1999，47，： | 1 |
| 13 | Modeled analysis of the biophysical nature of spectral shift and comparison with information content of broad bands显示文摘 | Baret F Jacquemoud S Guyot G | 1992 | Remote Sensing of Environment1992，41，： | 1 |
| 14 | The mouth-feel properties of grape and apple proanthocyanidins in a wine-like edium显示文摘 | VIDAL S FRANCIS L GUYOT S | 2003 | Journal of the Science of Food and Agriculture2003，83，6： | 1 |
| 15 | Evaluation of adjunctive HPV tes- ting by Hybrid Capture II in women with minor cytological abnormali- ties for the diagnosis of CIN2/3 and cost comparison with colposcopy 显示文摘 | Guyot A Karim S Kyi MS | 2003 | BMC Infect Dis2003，3，1： | 1 |
| 16 | Modeled analysis of the biophysical nature of spectral shifts and comparison with information content of broad bands 显示文摘 | Baret F Jacquermoud S Guyot G | 1992 | Remote Sensing of Environment1992，41，23： | 1 |
| 17 | Texture Analy- sis Based on Local Analysis of the Bidimensional Empirical Mode Decomposition显示文摘 | Nunes J C Guyot S Del6chelle E | 2005 | Machine Vision and Applications2005，16，3： | 1 |
| 18 | Time trends in the treatment and survival of recurrences from colorectal can- cer显示文摘 | Guyot F Faivre J Manfredi S | 2005 | Ann 0ncol2005，16，5： | 1 |
| 19 | Hepatic fibrosis and cirrhosis:the (myo) fibroblastic cell subpopulations involved显示文摘 | Guyot C Lepreux S Combea C | | 0，，02： | 1 |
| 20 | Fluxes of dissolved and colloidal organic carbon, along the Purus and Amazonas rivers (Brazil) 显示文摘 | PATEL N MOUNIER S GUYOT J L | 1999 | The Science of the Total Environment1999，229，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员