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1413篇 您的检索式:作者名="S R SUN"
    题名 作者 年代 出处 被引量
1Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER 2008Chinese Science Bulletin2008,53,7:8
2Imaging pancreatobiliary ductal system with optical coherence tomography: A review显示文摘An accurate, noninvasive and cost-effective method of in situ tissue evaluation during endoscopy would be highly advantageous for the detection of dysplasia or early cancer and for identifying different disease stages. Optical coherence tomography(OCT) is a noninvasive, high-resolution(1-10 μm) emerging optical imaging method with potential for identifying microscopic subsurface features in the pancreatic and biliary ductal system. Tissue microstructure of pancreaticobiliary ductal system has been successfully imaged by inserting an OCT probe through a standard endoscope operative channel. High-resolution OCT images and the technique's endoscopic compatibility have allowed for the microstructural diagnostic of thepancreatobiliary diseases. In this review, we discussed currently available pancreaticobiliary ductal imaging systems to assess the pancreatobiliary tissue microstructure and to evaluate varieties of pancreaticobiliary disorders and diseases. Results show that OCT can improve the quality of images of pancreatobiliary system during endoscopic retrograde cholangiopancheatography procedure, which may be important in distinguishing between the neoplastic and non-neoplastic lesions.Mohammad S Mahmud Gray R May Mohammad M Kamal Ahmed S Khwaja Carry Sun Alex Vitkin Victor XD Yang 2013World Journal of Gastrointestinal Endoscopy2013,5,11:2
3Wind-blown origin of the Pliocene red clay formation in the central Loess Plateau 显示文摘Ding Z L Sun J M Liu T S Zhu R X Yang S L Guo B 1998Earth and Planetary Science Letters1998,161,:1
4Scrption of phenanthrene from water onto alumina coated with dlanionic surfactants 显示文摘Sun'S B Jaffe P R 1996Environmental Science Technology1996,30,:1
5Support vector machine approach for protein subcellular localization prediction 显示文摘Hua S J Sun Z R 2001Bioinformatics2001,17,8:1
6Effects of epitopes com-bination and adjuvants on immune responses to anti-Alzhei-mer disease DNA vaccines in mice显示文摘HE Y SUN S H CHEN R W 2005Alzheimer Dis AssocDisord2005,19,4:1
7Linear theory and algorithms显示文摘LI S Y R SUN Q T Shao Ziyu Proceedings of the0,,3:1
8Calculation of energy losses in a distribution system显示文摘SUN D I ABE S SHOULTS R R 1980IEEE Trans on Power Apparatus and Systems1980,99,4:1
9SRAM based reprogrammable FPGA for space applications显示文摘WANG J J KATZ R B SUN J S 0,,06:1
10An approach to B-rep model sim- plification based on region suppression显示文摘Sun R Gao S M W Zhao 2010Computers & Graphics2010,34,5:1
11Oxidation behavior and breakdown of an aluminide coating on DZ40M alloy at high temperatures显示文摘Liu P S Liang K M Zhou H Y Guan H R Sun X F Jin T Yang K N 2001Oxidation of Metals2001,55,56:1
12The synthesis and electrochemicalperformance of LiV3 08 cathode with lanthanum - doped 显示文摘Hu F Sun M R Wu Y S 2012AdvancedMaterials Research2012,860,:1
13Heterotrimeric G proteins regulate nitrogen-use efficiency in rice 显示文摘Sun H Y Qian Q Wu K Luo J J Wang S S Zhang C W Ma Y F Liu Q Huang X Z Yuan Q B Han R X Zhao M Dong G J Guo L B Zhu X D Gou Z H Wang W Wu Y J Lin H X Fu X D 2014Nature Genetics2014,46,4:1
14RNA editing of transcripts of a chimeric mitochondrial gene associated with cytoplasmic male- sterility in Brassica显示文摘Stahl R Sun S L'Hornme Y 1994Nucleic acids research1994,22,11:1
15Ductile titanium alloy with low poission's ratio显示文摘HAO Y L LI S J SUN B B SUI M L YANG R 2007Phys Rev Lctt2007,98,21:1
16Instantaneous spectral analysis: Detection of low-frequency shadows associated with hydrocarbons 显示文摘CASTAGNA J P SUN S SIEGFRIED R W 2003The Leading Edge2003,22,2:1
17Predic tion of effective thermo-mechanical properties of par tieulate composites显示文摘Annapragada S R Sun D W Garimella S V 2007Computational Materials Sci ence2007,40,:1
18Cadmium accumulation in relation to organic acids and nonprotein thiols in leaves ofrecently found Cd hyperaccumulator Rorippa globosa and the Cd-accumulating plant Rorippa islandica显示文摘Sun R L Zhou Q X Wei S H 2011Journal ofPlant Growth Regulation2011,30,1:1
19Electronic properties of hydrogenated amorphous silicon-germanium alloys and long-range potential fluctuations 显示文摘Sheng S R Sun G S etal 2002Materials Science and Engineering2002,325,:1
20Trace Deter- ruination of Triclosan and Triclocarban in Environ- mental Water Sar0ples with Ionic Liquid Dispersive Liquid-phase Microextraction Prior to HPLC-ESI- MS-MS显示文摘ZHAO R S WANG X SUN J etal 2010Anal Bioanal Chem2010,397,4:1
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