| 2 | Development and validation of RP-HPLC and RP-UPLC methods for quantification of parathyroid hormones (1-34) in medicinal product formulated with meta-cresol显示文摘Rapid and sensitive reversed phase high performance liquid chromatography (RP-HPLC) and ultra performance liquid chromatography (RP-UPLC) method with UV detection has been developed and validated for quantification of parathyroid hormone (PTH) in presence of meta-cresol as a stabilizer in a pharmaceutical formulation.Chromatography was performed with mobile phase containing 0.1% Trifluoroacetic acid (TFA) in MilliQ water and 0.1% TFA in acetonitrile with gradient program and flow rate at 0.3 mL/min for HPLC and 0.4 mL/min for UPLC.Quantification was accomplished with internal reference standard (qualified against innovator product and National Institute for Biological Standards and Control (NIBSC) standard).The methods were validated for linearity (correlation coefficient 0.99),range,accuracy,precision and robustness.Robustness was confirmed by considering three factors;mobile phase composition,column temperature and flow rate/age of mobile phase.Intermediate precision was confirmed on different equipments,different columns and on different days.The relative standard deviation (RSD) (<2% for RP-HPLC and <1% for UPLC,n=30) indicated a good precision.Retention time was found about 17 min and 2 min by HPLC and UPLC methods,respectively.Both methods are simple,highly sensitive,precise and accurate and have the potential of being useful for routine quality control. | Shaligram S.Rane Alkesh Ajameri Rustom Mody P.Padmaja | 2012 | Journal of Pharmaceutical Analysis2012，2，2： | 1 |
| 3 | Lentil straw(Lens culinaris): An alternative and nutritious feed resource for kids显示文摘The scarcity of feed resources with the continuously increasing cost of usual animal feeds urgently demands searching some alternate feeds for ruminants. In this study, Barberi male kids were divided into4 groups of 5 kids(body weight 17.5 ± 1.8 kg) in each group, and ad libitum fed lentil straw(Lens culinaris;LS), LS based total mixed ration(LSTMR), urea ammoniated LS(ALS) or ALS based total mixed ration(ALSTMR) for a period of 28 days. Results showed LS was a superior feed(CP, 9.2%) for kids, but having quite high crude fibre(CF; 39.6%) and Ca:P ratio(10:1). Urea ammoniation of LS was helpful for increasing the digestible crude protein(DCP)(P < 0.01), nitrogen-free extract(NFE) and total digestible nutrients(TDN) with reduction of CF content. Urea mmoniation also improved the digestibility of neutral and acid detergent fibre(P < 0.01), but its effect on CP digestibility was negative(P < 0.05). Dry matter(DM), DCP and TDN intakes(per kg W0.75) were also improved(P < 0.01) in the kids fed ALS. Negative growth rate and nitrogen(N) balance(-33.8 and -1.4 g/day, respectively) in kids fed LS became positive(46.9 and 2.0 g/day, respectively) when ALS was used in the diets of kids. Feeding of ALS also increased(P < 0.01) the total N and ammonia N content of strained rumen liquor(SRL). Use of straw(LS or ALS) in TMR increased the digestibility of DM, organic matter and NFE(P < 0.01), intake of energy, as well as total volatile fatty acids concentration(P < 0.01) in the SRL. The present study suggested that optimum performance of kids may be achieved using either ALS alone or TMR with LS or ALS. | Vishal Mudgal Mukesh K.Mehta Ashok S.Rane | 2018 | Animal Nutrition2018，，4： | 0 |
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