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| 1 | Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression显示文摘AIM: To establish stable tetracycline-inducible pancreatic cancer cell lines. METHODS: Suit-2, MiaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-M2), under the control of either a cytomegalovirus (CMV) or a chicken β-actin promoter, and the resulting clones were characterised.RESULTS: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2E1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and Ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable. | Sarah Tonack Sabina Patel Mehdi Jalali Taoufik Nedjadi Rosalind E Jenkins Christopher Goldring John Neoptolemos Eithne Costello | 2011 | World Journal of Gastroenterology2011，17，15： | 4 |
| 2 | 6-Shogaol inhibits monosodium urate crystal-induced inflammation- an in vivo and in vitro study 显示文摘 | Sabina E P Rasool M Mathew L | 2010 | Food Chem Toxicol2010，48，1： | 1 |
| 3 | Lentivector-me-diated RNAi efficiently suppresses prion protein and prolongs surviv-al of scrapie-infected mice显示文摘 | ALEXANDER P SABINA E SABA A K | 2006 | J Clin Invest2006，116，12： | 1 |
| 4 | An in vivo and in vitro potential of italian ayurvedic herbal formulation triphala on experimental gouty arthritis in mice显示文摘 | Sabina E P Rasool M | 2008 | Fiseul Pharmacol2008，68，： | 1 |
| 5 | 显示文摘 | Todd E M Sabina Cemalovic Manna Warburton | 2006 | Sensor and Acurators B2006，116，： | 1 |
| 6 | Identification of a noncatalytic domain in AMP deaminase that influences binding to myosin显示文摘 | MARQUETANT R SABINA R L HOLMES E W | 1989 | Biochemistry1989，28，： | 1 |
| 7 | A role of piper- ine on monosodium urate crystal-induced inflammation-- an experimental model of gouty arthritis 显示文摘 | SABINA E P NAGAR S RASOOL M | 2011 | Inflamma- tion2011，34，3： | 1 |
| 8 | Regulation of skele tal muscle ATP catabolism by AMPD1 genotype during sprint exercise in asymptomatic subjects显示文摘 | NORMAN B SABINA R L JANSSON E | 2001 | J Appl Physiol2001，91，1： | 1 |
| 9 | 6-Shogao1 inhibits monosodium urate crystal-induced inflammation1 an in vivo and in vitro study 显示文摘 | Sabina E P Rasool M Mathew L | 2010 | Food Chem Toxicol2010，48，1： | 1 |
| 10 | An in vivo and in vitro potential of indian ayurvedic herbal formulation triphala on experimental gouty arthritis in mice 显示文摘 | Sabina E P Rasool M | 2008 | Vascul Pharmacol2008，48，： | 1 |
| 11 | Regulation of skeletal muscle ATP catabolism by AMPD1 genotype during sprint exercise in asymptomatic subjects显示文摘 | Norman B Sabina RL Jansson E | 2001 | J Appl Physiol2001，91，1： | 1 |
| 12 | Micrusomal triacylglycerol transfter protein in required for lumenal accretion oi triacylglycerol not associated with ApoB,as well as for ApoB iipidatioa显示文摘 | Agnes K Sabina R Vance-Jean E | 2002 | J Biol Chem2002，277，31： | 1 |
| 13 | Preparation and characterization of branched chitosans显示文摘 | Kristoffer T Ommeraas Sabina P Strand Bjf3rn E Christensen | 2011 | Carbohy Po- ly2011，83，15： | 1 |
| 14 | Regulation of skeletal muscle ATP catabolismby AMPDI genotype during sprint exercise in asymptomatic subjects显示文摘 | Norman B Sabina RL Jansson E | 2001 | J applPhysiol2001，91，1： | 1 |
| 15 | Muhisite modification of neomycin B: combined mitsunobu and click chemistry approach 显示文摘 | Sabina Quader Boyd S E Jenkins I D | 2007 | The Journal of Organic Chemistry2007，72，： | 1 |
| 16 | Regulation of skeletal muscle ATP catabolism by AMPD1 genotype during sprint exercise in asymptomatic subjects显示文摘 | Norman B Sabina R L Jansson E | 2001 | J Appl Physiol2001，91，1： | 1 |
| 17 | Chemical and irradiation treatments for killing Escherichia coli O157:H7 on alfalfa, radish, and mung bean seeds显示文摘 | Sari ML Nazuka E Sabina Y | 2003 | Journal of Food Protection2003，66，5： | 1 |
| 18 | 6-Shogaol inhibits monosodium urate crystal-induced inflammationAn in vivo and in vitro study显示文摘 | Sabina E P Rasool M Mathew L | 2010 | Food Chem Toxicol2010，48，1： | 1 |
| 19 | Replacement by Homologous Recombination of the minK Gene With lacZ Reveals Restriction of minK Expression to the Mouse Cardiac Conduction System 显示文摘 | Sabina Kupershmidt Tao Yang Mark E | 1999 | Circulation Research1999，84，： | 1 |
| 20 | Conductometric investigation of dye-surfactant ion pair formation in aqueous solution显示文摘 | SABINA Bracko JOZ E Span | 2000 | Dyes and Pigments2000，，45： | 1 |
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