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| 1 | Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC. | Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel | 2017 | World Journal of Stem Cells2017，9，9： | 4 |
| 2 | Method of delivery and pregnancy outcomes in Asia: the WHO global survey on maternal and perinatal health 2007–08显示文摘 | Pisake Lumbiganon Malinee Laopaiboon A Metin Gülmezoglu Jo?o Paulo Souza Surasak Taneepanichskul Pang Ruyan Deepika Eranjanie Attygalle Naveen Shrestha Rintaro Mori Nguyen Duc Hinh Hoang Thi Bang Tung Rathavy Kang Chuyun Kannitha Cheang Mario Festin Venus | 2010 | The Lancet2010，，9713： | 2 |
| 3 | Hydrogen as an additive to methane for spark ignition engine applications显示文摘 | Shrestha S O Karim G A | 1999 | International Journal of Hydrogen Energy1999，24，： | 1 |
| 4 | 显示文摘 | Shrestha R P Diyabalanage H V K Semelsberger T A | 2009 | Int J Hydrogen Energy2009，34，6： | 1 |
| 5 | Summer diets of wild and domestic ungulates in Nepal Himalaya显示文摘 | Shrestha R Wegge P Koirala R A | 2005 | Journal of Zoology2005，266，1： | 1 |
| 6 | Calcium anidotrihydroborate:a hydrogen storage material显示文摘 | Diyabalanage H V K Shrestha R P Semelsberger TA Scott B L Bowden M E Davis B L Burrell A K | | 0，，47： | 1 |
| 7 | Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease显示文摘 | UZAL F A FREEDMAN J C SHRESTHA A | 2014 | Future Microbiology2014，9，3： | 1 |
| 8 | Hydrogen as an additive to methane for spark ignition engine applications显示文摘 | Shrestha S O B Karim G A | 1999 | International Journal of Hydrogen Energy1999，，24： | 1 |
| 9 | The effect of tissue inhibitors on the antibacterial activity of chitosan nanoparticles and photodynamic therapy显示文摘 | Shrestha A Kishen A | 2012 | J Endod2012，38，9： | 1 |
| 10 | Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants 显示文摘 | Vanhercke T E I Tahchy A Shrestha P | 2013 | FEBS Lett2013，587，4： | 1 |
| 11 | Developmentally reg- ulated expression of Sema3A chemorepenant in the developing mouse incisor显示文摘 | Moe K Shrestha A Kvinnsland IH | 2012 | Acta Odontol Scand2012，70，3： | 1 |
| 12 | Congestion-driven trans- mission expansion in competitive power markets 显示文摘 | SHRESTHA G B FONSEKA P A J | 2004 | IEEE Transactions on Power Systems2004，19，3： | 1 |
| 13 | Pulsed elevated rate discharge of electrochemical energy storage devices显示文摘 | Shrestha B Wetz D A Novak P M | 2012 | Plasma Science2012，40，10： | 1 |
| 14 | Comparison of Lignocaine and Esmolol in attenuating cardiovascular response to laryngoscopy and endotracheal intubation显示文摘 | Shrestha A Acharya SP Amatya R | 2014 | Journal of Society of Anesthesiologists of Nepal2014，1，1： | 1 |
| 15 | Sinus histocytosis with massive lymphadenopathy (rosai-dorfman disease) : a ease report and literature review 显示文摘 | Pradhananga RB Dangol K Shrestha A | 2014 | Int Arch Otorhinlargngol2014，18，4： | 1 |
| 16 | Functional F C G R 2 B gene variantsinfluence Intravenous immunoglobulin response in patients with Kawasakidisease显示文摘 | Shrestha S W i e n e r H W Olson A K | | J Allergy Clin I m m u n o l0，128，3： | 1 |
| 17 | Dental caries prevalence,oral health knowledge and practice among indigenous Chepang school children of Nepal显示文摘 | Prasai Dixit L Shakya A Shrestha M | 2013 | BMC Oral Health2013，13，： | 1 |
| 18 | Factors affecting home delivery in the Kathmandu Valley,Nepal显示文摘 | Bolam A Manandhar Ds Shrestha P | 1998 | Health Policy Plan1998，13，： | 1 |
| 19 | Dental caries prevalence, oral health knowl- edge and practice among indigenous Chepang school children of Nepal 显示文摘 | Prasai Dixit L Shakya A Shrestha M | 2013 | BMC Oral Health2013，1413，1： | 1 |
| 20 | MicroRNA detection: challenges for the analytioal chemists显示文摘 | Cissell K A Shrestha S Deo S K D | 2007 | Anal Chem2007，79，： | 1 |
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