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| 1 | Titanium dioxide induced inflammation in the small intestine显示文摘AIM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (IL)-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P < 0.01); jejunum:NP 908.4 ± 130.3, MP 813.8 ± 103.8 vs 526.6 ± 61.43 (P < 0.05); and ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P < 0.05). In comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines IL-12, IL-4, IL-23, TNF-α, IFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):IL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P < 0.05); IL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P < 0.05); IL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P < 0.05); TNFα: NP 3.71 ± 1.33, MP 5.44 ± 1.67 vs 0.99± 019 (P < 0.05); IFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ± 0.69 (P < 0.05); and TGF-β: NP 780.70 ± 318.50, MP 1409.00 ± 502.20 vs 205.50 ± 63.93 (P < 0.05). CONCLUSION:Our findings indicate that TiO2 particles induce a Th1-mediated inflammatory response in the small bowel in mice. | Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan Dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello Iêda Nishitokukado Carmen Lúcia Ortiz-Agostinho Maria IrmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi | 2012 | World Journal of Gastroenterology2012，18，34： | 8 |
| 2 | Paradoxical increase in lumen size during progression of coronary atherosclerosis显示文摘 | SIPAHI L TUZCU E M SCHOENHAGEN P | 2006 | Atherosclerosis2006，189，1： | 1 |
| 3 | GES-type and OXA- 23 carbapenemase producing Aeinetobaeter baumannii in Turkey显示文摘 | Zeka A N Poirel L Sipahi O R | 2014 | JAntimicrob Chemother2014，69，4： | 1 |
| 4 | Effect of very highintensity statin therapy on regression of coronary atherosclerosis 显示文摘 | Nissen SE Nicholliss J Sipahi L | 2006 | JAMA2006，295，13： | 1 |
| 5 | Paradoxical increase in lumen size during progression of coronary atherosclerosis:observations from the REVERSAL trial显示文摘 | Sipahi L Tuzcu FM Schoenhagen P | 2006 | Atherosclerosis2006，189，1： | 1 |
| 6 | Coronary artery bypass grafting vs percutaneous coronary intervention and long-term mortality and morbid- ity in multivessel disease:meta-analysis of randomized clinical trials of the arterial grafting and stenting era显示文摘 | Sipahi l Akay MH Dagdelen S | 2014 | JAMA Intern Med2014，174，2： | 1 |
| 7 | Prevalence of celiac disease in an urban area of Brazil with predominantly European ancestry显示文摘AIM: To determine the prevalence of celiac disease in a group of volunteer blood donors at a blood bank in the city of Curitiba, Brazil through detection of the serum marker immunoglobulin A (IgA) antitransglutaminase an-tibody.METHODS: Blood samples collected from 2086 healthy subjects at the Paraná State Center for Hematology and Hemotherapy in Curitiba were submitted to ELISA testing for the IgA antitransglutaminase antibody. Positive samples received IgA antiendomysium antibody test through indirect immunofluorescence using human umbilical cord as substrate. Subsequently, patients who were positive on both tests underwent small bowel (distal duodenum) biopsy.RESULTS: Six subjects, four males and two females, tested positive for the two serum markers. Five of the six were submitted to intestinal biopsy (one declined the procedure). Biopsy results revealed changes in the distal duodenum mucosa (three classified as Marsh Ⅲb lesions and two as Marsh Ⅱ lesions). Most donors diagnosed having celiac disease presented multiple symptoms (gastrointestinal tract complaints). One donor reported having a family history of celiac disease (in a niece).CONCLUSION: Among apparently healthy blood donors,the prevalence of biopsy-confirmed celiac disease was approximately 1:417, similar to that seen in European countries. | Maria Angélica G Pereira Carmen L Ortiz-Agostinho Iêda Nishitokukado Maria N Sato Adérson OMC Damio Marília L Alencar Clarice P Abrantes-Lemos Eduardo LR Canado Thales de Brito Sérgio O Ioshii Sandra BM Valarini Aytan M Sipahi | 2006 | World Journal of Gastroenterology2006，12，40： | 1 |
| 8 | Effect of very high-intensitinity statin therapy on regression of coronary atherosclerosis: The ASTEROID Trail 显示文摘 | Nissen SE Nicholliss J Sipahi L | 2006 | JAMA2006，295，13： | 1 |
| 9 | GES-type and OXA-23 carbapenemase-prod ucing Acinetobacter baumannii in Turkey显示文摘 | Zeka AN Poirel L Sipahi OR | 2014 | Journal Antimicrob Che- mother2014，69，4： | 1 |
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