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| 1 | Clinical relevance of Helicobacter pylori babA2 and babA2/B in Costa Rica and Japan显示文摘AIM:To evaluate the prevalence of Helicobacter pylori(H.pylori ) babA2 ,babB and a recombinant gene between babA2 and babB(babA2/B ),and their role in the development of atrophic gastritis in Costa Rican and Japanese clinical isolates.METHODS:A total of 95 continuous H.pylori-positive Costa Rican(41 males and 54 females;mean age,50.65 years;SD,± 13.04 years) and 95 continuous H.pylori-positive Japanese(50 males and 45 females;mean age,63.43;SD,± 13.21 years) patients underwent upper endoscopy from October 2005 to July 2006.They were enrolled for the polymerase chain reaction(PCR)-based genotyping of the H.pylori babA2 ,babB and babA2/B genes.Statistical analysis was performed using the χ2 test and the Fisher's exact probability test and multivariate analysis was performed by logistic regression adjusting for gender and age.P < 0.05 was regarded as statistically significant.RESULTS:The PCR-based genotyping of 95 Costa Rican and 95 Japanese isolates showed a higher prevalence of babA2 in Japan(96.8%) than in Costa Rica(73.7%),while that of babA2/B was higher in Costa Rica(11.6%) than in Japan(1.1%).In Costa Rican isolates only,babA2 was significantly associated with atrophic gastritis(P = 0.01).CONCLUSION:These results suggest that the status of babA2 and babA2/B shows geographic differences,and that babA2 has clinical relevance in Costa Rica. | Sergio A Con Hiroaki Takeuchi Mitsuaki Nishioka Norihito Morimoto Tetsuro Sugiura Nobufumi Yasuda Reinaldo Con-Wong | 2010 | World Journal of Gastroenterology2010，16，4： | 4 |
| 2 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 3 | In vitro propagation of Japanese persimmon 显示文摘 | SUGIURA A TAO R MURAYAMA H | 1986 | Hortscience1986，21，5： | 2 |
| 4 | The origin of persimmon and its cultivar differentiation显示文摘 | Sugiura A | 1984 | Recent Advances in Plant Breeding1984，25，： | 1 |
| 5 | Effect of clenching oi1 biomechanical response of human mandi- hie and temporomandibu显示文摘 | MURAKAMI K YAMAMOTO K SUGIURA T A | 2013 | Medicina Oral Patnlogia Oral Ciru- gia Bucal2013，18，3： | 1 |
| 6 | Efects of day-length an d temperature on growth and bunch differentiation of grapevines显示文摘 | Utsunomiya N Kobayashi A | 1975 | J Japan S oc Hort Sci1975，43，： | 1 |
| 7 | Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus显示文摘 | Kobune F Sakata H Sugiura A | 1990 | J Virol1990，64，： | 1 |
| 8 | The geomagnetic field at 1982 from DE-2 and other magnetic field data 显示文摘 | Langel R A Ridgway J R Sugiura M | 1988 | Journal of Geomagnetism and Geoelectricity1988，40，9： | 1 |
| 9 | Analysis of phylogenetic relationships in Mangifera by restriction site analysis of an amplified region of cpDNA显示文摘 | Eiadthong W Yonemori K Sugiura A Utsunomiya N Subhadrabandhu S | 1999 | Scientia Horticuhurae1999，80，： | 1 |
| 10 | Simulation technology applied to vehicle development显示文摘 | Magoshi Y Fujisaw A H Sugiura T | 2003 | Auto Vehicle Technique2003，57，3： | 1 |
| 11 | Assessment of continuous positive airway pressure treatment in obstructive sleep apnea syndrome using 24-hour urinary catecholamines显示文摘 | Nods A Sugiura T | 2005 | Clin Cardiol2005，28，： | 1 |
| 12 | Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds 显示文摘 | Sugihara A Sugiura K Morita H | 2000 | Proc Soc Exp Biol Med2000，225，1： | 1 |
| 13 | Influence of nasal resistance on initial acceptance of continuous positive airway pressure in treatment for obstructive sleep syndrome显示文摘 | Sugiura T Noda A Nakata S | | 0，，01： | 1 |
| 14 | Molecular typing of S alleles through identifcation, characterization and cDNA cloning for S-RNases in sweet cherry显示文摘 | Tao R Yamane H Sugiura A | 1999 | J Amer Soc Hort Sci1999，124，： | 1 |
| 15 | Cognitive Training Changes Hippoeampal Function in Mild Cognitive Impairment: A Pilot Study显示文摘 | ROSEN A C SUGIURA L KRAMER J H | 2011 | J Alzheimers Dis2011，26，3： | 1 |
| 16 | Availability of phosphorus and trace elements in low-phytate varie- ties of barley and corn for rainbow trout Oncorhynchus mykiss显示文摘 | Sugiura S H Raboy V Young K A | 1999 | Aquaculture1999，170，34： | 1 |
| 17 | Size control of calcium alginate beads containing living cells using micro-nozzle array 显示文摘 | Sugiura S Oda T Izumida Y Aoyagi Y Satake M Ochiai A Ohkohchi N Nakajima M | 2005 | Biomaterials2005，26，16： | 1 |
| 18 | Identification of mango cuhivars of Thailand and evaluation of their genetic variation using the amplified fragments by simple sequence repeat(SSR) anchored primers显示文摘 | Eiadthong W Yonemori K Sugiura A | 1999 | Scentia Horticuhurae1999，82，： | 1 |
| 19 | Effective personalization of push-type systems:visualizing information freshness显示文摘 | SAKAGAMI H KAMBA T SUGIURA A | 1998 | Computer Networks and ISDN Systems1998，30，17： | 1 |
| 20 | TLR3 activationaugments matrix metalloproteinase production throughreactive nitrogen species generation in human lung fibroblasts显示文摘 | Ichikawa T Sugiura H Koarai A | 2014 | J Immunol2014，192，11： | 1 |
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