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| 1 | Correlation of p53 over-expression and alteration in p53 gene detected by polymerase chain reaction-single strand conformation polymorphism in adenocarcinoma of gastric cancer patients from India显示文摘AIM:To study the alterations in p53 gene among Indian gastric cancer patients and to correlate them with the various clinicopathological parameters. METHODS:A total of 103 gastric cancer patients were included in this study.The p53 alterations were studied by both immunohistochemical method as well as polymerase chain reaction(PCR)-single strand conformation polymorphism(SSCP)analysis.We only studied four(exon 5,6,7,and 8)of the 11 p53 exons.The alterations in p53 were also correlated with respect to various clinicopathological parameters. RESULTS:Among 103 cases,p53 over-expression and alteration were detected in 37(35.92%)and 19(18.44%)cases,respectively.Most of the p53 alterations were found at exon 5(31.54%),followed by exon 6(26.31%),exon 7(21.04%)and exon 8(21.04%).A significant correlation of p53 over- expression was found with p53 alteration(P=0.000). Concordance between p53 alteration(as detected by SSCP)and over-expression[as detected by immunohistochemistry(IHC)]was found in 75%cases. We found that IHC-positive/SSCP-negative cases accounted for 21%of cases and IHC-negative/SSCP- positive cases accounted for remaining 4%cases. CONCLUSION:Our results show that p53 genemutations are significantly correlated with p53 protein overxpression,with 75%concordance in overexpression and alteration in the p53 gene,but 25% disconcordance also cautions against the assumption that p53 over-expression is always associated with a gene mutation.There may be other mechanisms responsible for stabilization and accumulation of p53 protein with no evidence of gene mutation that reflect an accumulation of a non-mutated protein,or a false negative SSCP result. | Sajjad Karim Arif Ali | 2009 | World Journal of Gastroenterology2009，15，11： | 28 |
| 2 | 甲醛诱导Tau蛋白形成“孔道样”聚集结构显示文摘尽管Lin等(University of California,Santa Barbara)就蛋白构象病中细胞死亡的机制提出了'非特异性淀粉样离子通道'(aspecific amyloid ion channels)学说,但到目前为止,尚未发现神经Tau蛋白能形成'孔道样'聚集结构,也未寻找到可以导致蛋白质形成'孔道样'聚集结构的诱导剂.依据本实验室提出的'散发性老年痴呆发生发展中的内源性甲醛慢性损伤'假说,采用一定浓度的甲醛与Tau蛋白进行温育,观察到甲醛可以明显诱导Tau蛋白分子聚集并形成淀粉样沉积物,同时也观察到了Tau蛋白'孔道样'聚集结构.上述结果为探索甲醛诱导Tau蛋白错误折叠形成的产物导致细胞代谢障碍和死亡的机制提供了新的研究思路. | NAQVI Sajjad Haider 王维山 苗君叶 赫荣乔 | 2010 | 生物化学与生物物理进展2010，37，11： | 21 |
| 3 | Using Path Analysis to Identify the Influence of Climatic Factors on Spring Peak Flow Dominated by Snowmelt in an Alpine Watershed显示文摘Spring snowmelt peak flow(SSPF) can cause serious damage. Precipitation as rainfall directly contributes to the SSPF and influences the characteristics of the SSPF, while temperature indirectly impacts the SSPF by shaping snowmelt rate and determining the soil frozen state which partitions snowmelt water into surface runoff and soil infiltration water in spring. It is necessary to identify the important and significant paths of climatic factors influencing the SSPF and provide estimates of the magnitude and significance of hypothesized causal connections between climatic factors and the SSPF.This study used path analysis with a selection of five factors – the antecedent precipitation index(API),spring precipitation(SP), winter precipitation as snowfall(WS), <0°C temperature accumulation in winter(|ATN|), and average >0°C temperature accumulation in spring(AT) – to analyze their influences on the SSPF in the Kaidu River in Xinjiang,China. The results show that |ATN|, AT and WS have a significant correlation with the SSPF, while API and SP do not show a significant correlation. AT and WS directly influence the SSPF, while as the influence of |ATN| on SSPF is indirect through WS and AT. The indirect influence of |ATN| on SSPF through WS accounts for 69% of the total influence of |ATN| on SSPF. Compared to the multiple linear regression method, path analysis provides additional valuable information, including influencing paths from independent variables to the dependent variable as well as direct and indirect impacts of external variables on the internal variable. This information can help improve the description of snow melt and spring runoff in hydrologic models as well as the planning and management of water resources. | ZHANG Fei-Yun LI Lan-Hai AHMAD Sajjad LI Xue-Mei | 2014 | Journal of Mountain Science2014，11，4： | 16 |
| 4 | APHRODITE降水数据驱动的融雪径流模拟显示文摘针对流域内气象观测站点稀少和融雪径流过程的特点,利用APHRODITE降水数据进行插值,应用日有效活动温度改进度日数;依据季节性冻土受有效活动积温影响的特点,建立有效活动积温与径流系数的关系,提高模型中融雪速率和径流系数的计算精度。结合气象、水文资料和MODIS遥感积雪产品,应用改进的融雪径流模型(SRM)对开都河流域2000年与2006年融雪期的径流进行了率定和验证模拟。改进模型在率定期和验证期的模拟结果远远优于用日平均温度作为度日数的结果。结果表明,用APHRODITE降水数据及改进的度日数和径流系数作为SRM模型参数输入,能够较好模拟开都河流域融雪径流过程,大大提高模型模拟精度。 | 李兰海 尚明 张敏生 AHMAD Sajjad 黄粤 | 2014 | 水科学进展2014，25，1： | 11 |
| 5 | 氮肥及硝化抑制剂配合施用对石灰性土壤二氧化碳释放的影响显示文摘为研究施用氮肥对同时含有有机碳及无机碳石灰性土壤碳释放的影响,在陕西杨凌进行田间试验,比较了不同施氮量(0、160、220 kg·hm^(-2))及其与硝化抑制剂(DCD)配合(N160、N220及N160+DCD、N220+DCD)对土壤pH值、矿质态氮含量和二氧化碳(CO_2)释放量的影响。结果表明:施用氮肥显著降低了耕层土壤pH;配施DCD后土壤pH降低幅度小于未加DCD处理;加入DCD使氮肥的硝化过程推迟了约20 d;未加DCD处理的土壤CO_2释放量随施氮量增加而增加,试验结束时(施肥37 d后)土壤CO_2累积释放量最高达到167.1 g·m^(-2)。与N0处理相比,N160和N220处理的土壤CO_2累积释放量显著增加,增幅分别为20.9%和25.7%;N160+DCD和N220+DCD处理显著降低了土壤CO_2累积释放量,比对应相同施氮量处理分别降低了13.5%和11.0%。上述结果说明施用氮肥会同时影响石灰性土壤有机碳及无机碳的释放,施用氮肥引起的土壤无机碳的释放值得关注。 | 李雪松 Sajjad Raza 刘占军 陈竹君 周建斌 | 2017 | 农业环境科学学报2017，36，8： | 11 |
| 6 | Abrasive Wear Behavior of High Chromium Cast Iron and Hadfield Steel-A Comparison显示文摘Wear properties of two different crushers used for grinding raw materials of cement industry are compared using pin-on-disk wear test.The wear test was carried out with different loads on a pin.Abrasive wear behavior of two alloys was evaluated by comparing mass loss,wear resistance,microhardness and friction coefficient.The microstructure of the specimens was detected using optical microscope.The results showed that abrasive wear of high chromium cast iron is lower than that of Hadfield steel.Due to the presence of M7C3 carbides on the high chromium cast iron matrix,impact crushers exhibited higher friction coefficient. | Mehdi Mazar Atabaki Sajjad Jafari Hassan Abdollah-pour | 2012 | Journal of Iron and Steel Research(International)2012，19，4： | 10 |
| 7 | An analytical model for estimating rock strength parameters from small-scale drilling data显示文摘The small-scale drilling technique can be a fast and reliable method to estimate rock strength parameters. It needs to link the operational drilling parameters and strength properties of rock. The parameters such as bit geometry, bit movement, contact frictions and crushed zone affect the estimated parameters.An analytical model considering operational drilling data and effective parameters can be used for these purposes. In this research, an analytical model was developed based on limit equilibrium of forces in a Tshaped drag bit considering the effective parameters such as bit geometry, crushed zone and contact frictions in drilling process. Based on the model, a method was used to estimate rock strength parameters such as cohesion, internal friction angle and uniaxial compressive strength of different rock types from operational drilling data. Some drilling tests were conducted by a portable and powerful drilling machine which was developed for this work. The obtained results for strength properties of different rock types from the drilling experiments based on the proposed model are in good agreement with the results of standard tests. Experimental results show that the contact friction between the cutting face and rock is close to that between bit end wearing face and rock due to the same bit material. In this case,the strength parameters, especially internal friction angle and cohesion, are estimated only by using a blunt bit drilling data and the bit bluntness does not affect the estimated results. | Sajjad Kalantari Alireza Baghbanan Hamid Hashemalhosseini | 2019 | Journal of Rock Mechanics and Geotechnical Engineering2019，11，1： | 9 |
| 8 | 沼液替代化肥对甜瓜产量品质及养分吸收的影响显示文摘以'绿宝石'薄皮甜瓜为试材,采用田间小区随机区组试验,设置5个处理(CK:不施肥,CF:常规施肥,FR:化肥减量,BS:沼液替代,BR:沼液减量),研究了集约化养猪场粪肥和废水发酵后的沼液通过水肥一体化管道施用替代化肥对日光温室栽培甜瓜产量、品质及养分吸收的影响。结果表明:1)不同处理间甜瓜根、茎、叶、果实的干物质量和养分含量及养分携出量均无显著差异,甜瓜温室栽培下每形成1 000 kg经济产量对N、P2O5、K2O吸收量分别为4.16~4.68、1.04~1.36、7.07~7.85 kg,N、P2O5、K2O需求比例平均为1∶0.30∶1.72。2)不同处理间甜瓜单果质量和产量均无显著差异;BS、BR处理甜瓜可溶性总糖含量、可溶性固形物含量、糖酸比较CF处理显著提高。3)BS、BR和FR处理节本增收效果显著,较CF处理分别增效26.77%、8.49%和3.37%;BR和FR处理N、P、K养分利用率较CF处理分别提高12.47%、9.84%、15.26%和11.10%、9.62%、41.47%。综上,沼液水肥一体化可以完全替代化肥追施,实现沼液资源化利用。 | 高旭 孔祥俊 郭雨浓 李佳聪 SAJJAD Raza 陈竹君 | 2019 | 北方园艺2019，，14： | 8 |
| 9 | Deep learning approach based on dimensionality reduction for designing electromagnetic nanostructures显示文摘In this paper,we demonstrate a computationally efficient new approach based on deep learning(DL)techniques for analysis,design and optimization of electromagnetic(EM)nanostructures.We use the strong correlation among features of a generic EM problem to considerably reduce the dimensionality of the problem and thus,the computational complexity,without imposing considerable errors.By employing the dimensionality reduction concept using the more recently demonstrated autoencoder technique,we redefine the conventional many-to-one design problem in EM nanostructures into a one-to-one problem plus a much simpler many-to-one problem,which can be simply solved using an analytic formulation. | Yashar Kiarashinejad Sajjad Abdollahramezani Ali Adibi | 2020 | npj Computational Materials2020，，1： | 8 |
| 10 | 863 genomes reveal the origin and domestication of chicken显示文摘Despite the substantial role that chickens have played in human societies across the world,both the geographic and temporal origins of their domestication remain controversial.To address this issue,we analyzed 863 genomes from a worldwide sampling of chickens and representatives of all four species of wild jungle fowl and each of the five subspecies of red jungle fowl(RJF).Our study suggests that domestic chickens were initially derived from the RJF subspecies Gallus gallus spadiceus whose present-day distribution is predominantly in southwestern China,northern Thailand and Myanmar.Following their domestication,chickens were translocated across Southeast and South Asia where they interbred locally with both RJF subspecies and other jungle fowl species.In addition,our results show that the White Leghorn chicken breed possesses a mosaic of divergent ancestries inherited from other subspecies of RJF.Despite the strong episodic gene flow from geographically divergent lineages of jungle fowls,our analyses show that domestic chickens undergo genetic adaptations that underlie their unique behavioral,morphological and reproductive traits.Our study provides novel insights into the evolutionary history of domestic chickens and a valuable resource to facilitate ongoing genetic and functional investigations of the world's most numerous domestic animal. | Ming-Shan Wang Mukesh Thakur Min-Sheng Peng Yu Jiang Laurent Alain Francois Frantz Ming Li Jin-Jin Zhang Sheng Wang Joris Peters Newton Otieno Otecko Chatmongkon Suwannapoom Xing Guo Zhu-Qing Zheng Ali Esmailizadeh Nalini Yasoda Hirimuthugoda Hidayat Ashari Sri Suladari Moch Syamsul Arifin Zein Szilvia Kusza Saeed Sohrabi Hamed Kharrati-Koopaee Quan-Kuan Shen Lin Zeng Min-Min Yang Ya-Jiang Wu Xing-Yan Yang Xue-Mei Lu Xin-Zheng Jia Qing-Hua Nie Susan Joy Lamont Emiliano Lasagna Simone Ceccobelli Humpita Gamaralalage Thilini Nisanka Gunwardana Thilina Madusanka Senasige Shao-Hong Feng Jing-Fang Si Hao Zhang Jie-Qiong Jin Ming-Li Li Yan-Hu Liu Hong-Man Chen Cheng Ma Shan-Shan Dai Abul Kashem Fazlul Haque Bhuiyan Muhammad Sajjad Khan Gamamada Liyanage Lalanie Pradeepa Silva Thi-Thuy Le Okeyo Ally Mwai Mohamed Nawaz Mohamed Ibrahim Megan Supple Beth Shapiro Olivier Hanotte Guojie Zhang Greger Larson Jian-Lin Han Dong-Dong Wu Ya-Ping Zhang | 2020 | Cell Research2020，30，8： | 7 |
| 11 | 药用植物内生菌对作物生长及氧化应激的作用(英文)显示文摘目的:探讨药用植物内生菌的多样性及其在种子生长和氧化应激中的作用。方法:从三种药用植物(Caralluma acutangula、Rhazya stricta和Moringa peregrina)中提取内生菌;基于18S r DNA测序和系统发育分析鉴定分离得到的内生菌株;以正常和矮化突变体水稻品系为对照,比较不同浓度的内生菌培养滤液(CF)对水稻种子的发芽和生长的影响;通过气相色谱-质谱分析CF中的有效活性成分。结论:从药用植物中共获得10种内生菌,包括茎点霉属6株、链格孢属2株、双极霉属1株和枝孢霉属1株。CF表现出剂量依赖性的生长刺激和抑制作用。与对照和其他内生菌相比,100%的茎点霉菌CF显著促进了水稻种子的发芽和生长;双极霉中的吲哚乙酸含量最高,并表现出比其更高的自由基清除和抗脂质过氧化活性;双极霉菌和茎点霉菌的类黄酮和酚类成分较高。综上所述,药用植物中存在内生菌株,其可以用于改善作物生长和减轻氧化应激。 | Abdul Latif KHAN Syed Abdullah GILANI Muhammad WAQAS Khadija AL-HOSNI Salima AL-KHIZIRI Yoon-Ha KIM Liaqat ALI Sang-Mo KANG Sajjad ASAF Raheem SHAHZAD Javid HUSSAIN In-Jung LEE Ahmed AL-HARRASI | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，2： | 6 |
| 12 | Evaluating effectiveness of frequency ratio, fuzzy logic and logistic regression models in assessing landslide susceptibility: a case from Rudraprayag district, India显示文摘Rudraprayag in Garhwal Himalayan division is one of the most vulnerable districts to landslides in India. Heavy rainfall, steep slope and developmental activities are important factors for the occurrence of landslides in the district. Therefore, specific assessment of landslide susceptibility and its accuracy at regional level is essential for disaster management and proper land use planning. The article evaluates effectiveness of frequency ratio, fuzzy logic and logistic regression models for assessing landslide susceptibility in Rudraprayag district of Uttarakhand state, India. A landslide inventory map was prepared and verified by field data. Fourteen landslide parameters and generated inventory map were utilized to prepare landslide susceptibility maps through frequency ratio, fuzzy logic and logistic regression models. Landslide susceptibility maps generated through these models were classified into very high, high, medium, low and very low categories using natural breaks classification. Receiver operating characteristics(ROC) curve, spatially agreed area approach and seed cell area index(SCAI) method were used to validate the landslide models. Validation results revealed that fuzzy logic model was found to be more effective in assessing landslide susceptibility in the study area. The landslide susceptibility map generated through fuzzy logic model can be best utilized for landslide disaster management and effective land use planning. | Mehebub SAHANA Haroon SAJJAD | 2017 | Journal of Mountain Science2017，14，11： | 6 |
| 13 | Nb添加对Nd-Ce-Fe-B合金的磁性能及晶间交换耦合作用的影响显示文摘研究了不同含量的Nb添加对(Nd0.5Ce0.5)13Fe82-xNbxB5快淬合金的磁性能、物相组成、交换耦合作用及其初始磁化机制的影响.发现Nb添加能抑制α-Fe的形成,显著改善合金的磁性能.当Nb的添加量(原子分数)为0.5%时,合金获得较优的磁性能.随着Nb的添加量(原子分数)从0增加到0.5%,合金的矫顽力由未添加时的777 kA/m提升到900 kA/m,最大磁能积也从未添加Nb时的64 kJ/m^3增加到78 kJ/m^3,而合金的剩磁仅由未添加Nb时的0.71 T轻微下降到0.70 T.合金中晶粒间的交换耦合作用随着Nb的添加而增强.合金的初始磁化机制也有了显著的变化. | 何伦可 权其琛 胡贤君 江庆政 Sajjad Ur Rehman 钟明龙 黎业生 | 2018 | 有色金属科学与工程2018，9，5： | 6 |
| 14 | Bonded-particle model calibration using response surface methodology显示文摘 | Sajjad Chehreghani Mohammad Noaparast Bahram Rezai Sied Ziaedin Shafaei | 2017 | Particuology2017，15，3： | 6 |
| 15 | Differential gene expression of chemokines in KRAS and BRAF mutated colorectal cell lines:Role of cytokines显示文摘AIM:To study KRAS/BRAF mutations in colorectalcancer(CRC)that influences the efficacy of treatment.To develop strategies for overcoming combination of treatment.METHODS:Five colonic cell-lines were investigated:DLD-1 with KRAS(G13D)mutation,HT 29 and Colo205 with BRAF(V600E)mutation as well as the wild type(Wt)cell-lines Caco2 and Colo-320.DLD-1(KRAS),HT-29(BRAF)and Caco2(Wt)cell lines were treated with cytokines(TNFα50 ng,IL-1β1 ng and IFNγ50ng)and harvested at different time points(1-24 h).KRAS inhibition was performed by the siRNA-approach.Two colorectal cancer cells DLD-1 and Caco2 were used for KRAS inhibition.About 70%confluency were confirmed before transfection with small interferring RNA(siRNA)oligonucleotides.All the synthetic siRNA sequences were designed in our laboratory.Total RNA and protein was isolated from the cells for RT-PCR and Western blotting.Densitometry of the Western blotting was analyzed with the Image J software(NIH).Results are shown as mean±SD.RESULTS:RT-PCR analysis in non-stimulated cells showed a low basal expression of TNFαand IL-1βin the DLD-1 KRAS-mutated cell-line,compared to Caco2wild type.No detection was found for IL-6 and IFNγin any of the studied cell lines.In contrast,pro-angiogenic chemokines(CXCL1,CXCL8)showed a high constitutive expression in the mutated cell-lines DLD-1(KRAS),HT-29 and Colo205(BRAF),compared to wild type(Caco2).The anti-angiogenic chemokine(CXCL10)showed a high basal expression in wild-type,compared to mutated cell-lines.KRAS down-regulation by siRNA showed a significant decrease in CXCL1 and CXCL10gene expression in the DLD-1(KRAS)cell-line in comparison to wild type(Caco2)at 72 h after KRAS silencing.In contrast,the specific KRAS inhibition resulted in an up-regulation of CXCL1 and CXCL10.The results of our study show a higher expression of pro-angiogenic chemokines at basal level in mutated cell-lines,which was further increased by cytokine treatment.CONCLUSION:To summarize,basal chemokine gene expression for pro-angiogenic chemokines was high in mutated as compared to wild type cell-lines.This reflects the likely existence of a different microenvironment in tumours consistent of wild type or mutated cells.This may help to rationalize the choice of molecular targets for suitable therapeutic investigation in clinical studies. | Sajjad Khan Silke Cameron Martina Blaschke Federico Moriconi Naila Naz Ahmad Amanzada Giuliano Ramadori Ihtzaz Ahmed Malik | 2014 | World Journal of Gastroenterology2014，20，11： | 6 |
| 16 | Hepatitis D: Scenario in the Asia-Pacifi c region显示文摘Hepatitis D virus (HDV) infection is present worldwide and affects all age groups. Around 18 million people are estimated to be infected with HDV. An important trend in HDV infection is global decline. HDV prevalence has decreased significantly in Europe since the 1970s and 1980s when it was f irst reported. The Asia-Pacif ic region now seems to be where HDV is a major health concern. There is a lack of available data from most of the countries from this region; hence, the true status of HDV cannot be determined. In South Asia, most of the countries have conditions that are favorable for the spread of hepatitis B and other related infections. Countries like Pakistan and Iran have shown an increase in HDV prevalence over a period of time. Other countries and region like China, Turkey, Australia, Japan, India and Taiwan, some of which had very high HDV prevalence in the past, have shown a decline in the incidence, but high prevalence persists in some. Intravenous drug abusers, homosexual men and women, prostitutes, and people on hemodialysis are the groups with very high HDV prevalence. | Zaigham Abbas Wasim Jafri Sajjad Raza | 2010 | World Journal of Gastroenterology2010，16，5： | 6 |
| 17 | 基于空间谱的多天线盲频谱感知算法显示文摘现有的频谱感知算法主要在时间、频率以及地理空间维度进行检测,对角度维的利用尚不成熟。将多天线技术中的到达角(AOA,angle of arrival)估计算法应用到频谱感知领域,提出了2种基于空间谱的盲频谱感知算法,分别为最大—最小延迟相加谱值比检测和平均—最小延迟相加谱值比检测。利用空域匹配滤波的优势,新算法在低信噪比下得到了较高的检测概率,同时为角度维的频谱接入提供了AOA信息,从而提高了频谱利用率。此外,运用随机矩阵理论,推导了检测阈值和检测概率的理论值。仿真结果表明,在Nakagami-m信道下,提出的算法具有比现有盲感知算法更优的检测性能。 | 刘畅 Syed Sajjad Ali 张锐 李思莹 王洁 金明录 | 2015 | 通信学报2015，36，4： | 6 |
| 18 | Eh耦合的稻田土壤镉、砷释放机制显示文摘镉(Cd)、砷(As)是稻田土壤的主要重(类)金属污染物,稻田土壤Cd、As的释放与其氧化还原电位(Eh)密切相关,但Eh耦合的稻田土壤砷、镉释放机制仍不清楚.本实验利用高精度土壤微反应器模拟Eh梯度变化过程(-250、-50、170 mV),研究其耦合的土壤Cd、As及相关元素(Fe、Al、Ca、S、P、DOC)的释放特征,并结合同步辐射微区X射线荧光光谱技术(μ⁃XRF)探讨了不同Eh(-250、-50、170 mV)条件下稻田土壤As释放机制.结果表明:随着供试体系Eh从-250 mV升高至170 mV,土水混合液中DOC和pH值逐步下降,但Cd、As及相关Al、S、P、Fe元素浓度则同时升高.其中,Cd与S、Al增长均呈先缓后快特征,而As与P、Fe增长则呈先快后缓趋势,说明在Eh升高过程中Cd与S,以及As与Fe间密切相关.同步辐射μ⁃XRF分析发现,在低Eh条件下,土壤颗粒上As、Fe和S的高含量点信号重合,As⁃S及As⁃Fe的可决系数分别在Eh为-250 mV和170 mV条件下最高,说明在低Eh条件下As很可能主要受硫化铁控制,但在高Eh条件下则受铁氧化物影响.综上可知,低Eh条件下,As、Cd主要受到硫化铁的控制.随着Eh的升高,土壤Cd的活性逐渐升高,As的活性则受到铁氧化物控制.本研究结果可为理解土壤Cd、As共同释放机制及调控土壤Cd、As活性提供重要理论依据. | 张良东 杨建军 夏星 Sajjad Hussain | 2020 | 环境科学学报2020，40，5： | 6 |
| 19 | 焊丝对工业纯铜和304不锈钢钨极氩弧焊接的影响(英文)显示文摘采用不同焊丝对工业纯铜和304不锈钢进行钨极氩弧焊接。结果表明,采用铜做焊丝时,焊缝无任何缺陷生成,而采用304不锈钢和Ni-Cu-Fe合金为焊丝材料时,焊缝中有凝固裂纹和未熔化区存在。在最优条件下,焊缝的抗拉强度能达到铜材的96%。焊缝在弯曲到180°下也没有分离、撕裂和断裂等现象发生。这表明铜是一种较好的工业纯铜与304不锈钢GTA焊的焊丝材料。 | Sajjad Gholami SHIRI Mohsen NAZARZADEH Mahmood SHARIFITABAR Mehdi Shafiee AFARANI | 2012 | Transactions of Nonferrous Metals Society of China2012，22，12： | 5 |
| 20 | CRISPR/Cas9-mediated PBP1 and PBP3 mutagenesis induced significant reduction in electrophysiological response to sex pheromones in male Chilo suppressalis显示文摘Pheromone-binding proteins (PBPs) are thought to bind and transport sex pheromones onto the olfactory receptors on the dendrite membrane of olfactory neurons, and thus play a vital role in sex pheromone perception. However, the function of PBPs has rarely been demonstrated in vivo.In this study, two PBPs (PBP1 and PBP3) of Chilo suppressalis, one of the most notorious pyralid pests, were in vivo functionally characterized using insects with the PBP gene knocked out by the CRISPR/Cas9 system. First, through direct injection of PBP-single guide RNA (sgRNA)/Cas9 messenger RNA into newly laid eggs, a high rate of target-gene editing (checked with polled eggs) was induced at 24 h after injection, 21.3% for PBPl-sgRNA injected eggs and 19.5% for PBP3-sgRNA injected eggs. Second, by an in-crossing strategy, insects with mutant PBP1 or PBP3 (both with a premature stop codon) were screened and homozygous mutants were obtained in the G3 generation. Third, the mutant insects were measured for electroantennogram (EAG) response to female sex pheromones. As a result, both PBP mutant males displayed significant reduction in EAG response, and this reduction in PBP1 mutants was higher than that in PBP3 mutants, indicating a more important role of PBP1. Finally, the relative importance of two PBPs and the possible off target effect induced by sgRNA-injection are discussed. Taken together, our study provides a deeper insight into the function of and interaction between different PBP genes in sex pheromone perception of C. suppressalis, as well as a valuable reference in methodology for gene functional study in other genes and other moth species. | Xiao-Tong Dong Hui Liao Guan-Heng Zhu Sajjad Ali Khuhro Zhan-Feng Ye Qi Yan Shuang-Lin Dong | 2019 | Insect Science2019，26，3： | 5 |
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