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1篇 您的检索式:作者名="Samar O.Rabah"
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1Passiflora plastome sequencing reveals widespread genomic rearrangements显示文摘Although past studies have included Passiflora among angiosperm lineages with highly rearranged plastid genomes (plastomes),knowledge about plastome organization in the genus is limited.So far only one draft and one complete plastome have been published.Expanded sampling of Passiflora plastomes is needed to understand the extent of the genomic rearrangement in the genus,which is also unusual in having biparental plastid inheritance and plastome-genome incompatibility.We sequenced 15 Passiflora plastomes using either Illumina paired-end or shotgun cloning and Sanger sequencing approaches.Assembled plastomes were annotated using Dual Organellar GenoMe Annotator (DOGMA)and tRNAscan-SE.The Populus trichocarpa plastome was used as a reference to estimate genomic rearrangements in Passifloru by performing whole genome alignment in progressiveMauve.The phylogenetic distribution of rearrangements was plotted on the maximum likelihood tree generated from 64 plastid encoded protein genes.Inverted repeat (IR)expansion/contraction and loss of the two largest hypothetical open reading frames,ycf1 and ycf2,account for most plastome size variation,which ranges from 139 262 base pairs (bp)in P.biflora to 161 494 bp in P.pittieri.Passifloru plastomes have experienced numerous inversions,gene and intron losses along with multiple independent IR expansions and contractions resulting in a distinct organization in each of the three subgenera examined.Each Passiflora subgenus has a unique plastome structure in terms of gene content,order and size.The phylogenetic distribution of rearrangements shows that Passiflora has experienced widespread genomic changes,suggesting that such events may not be reliable phylogenetic markers.Samar O.Rabah Bikash Shrestha Nahid H.Hajrah Mumdooh J.Sabir Hesham F.Alharby Mernan J.Sabir Alawiah M.Alhebshi Jamal S.M.Sabir Lawrence E.Gilbert Tracey A.Ruhlman Robert K.Jansen 2019Journal of Systematics and Evolution2019,57,1:2
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