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| 1 | Converting lateral scanning into axial focusing to speed up three-dimensional microscopy显示文摘In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging.Recently,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been achieved.However,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging.In an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging.Using a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image space.We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three dimensions.We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution. | Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.Kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka | 2020 | Light(Science & Applications)2020，9，1： | 4 |
| 2 | CEACAM1 in malig- nant melanoma : a diagnostic and therapeutic target显示文摘 | Sapoznik S Ortenberg R Schachter J | 2012 | Curr Top Med Chem2012，12，1： | 1 |
| 3 | Gonadotropin-regulated lymphangiogenesis in ovarian cancer is mediated by LEDGF-induced expression of VEGF-C显示文摘 | Sapoznik S Cohen B Tzuman Y | | 0，，24： | 1 |
| 4 | Novel an- ti-melanoma immunotherapies: disarming tumor escape mechanisms显示文摘 | Sapoznik S Hammer O Ortenberg R | 2012 | Clin Dev Immunol2012，8，18： | 1 |
| 5 | CEACAM1 in malignant melanoma: a diagnostic and therapeutic target 显示文摘 | Sapoznik S Ortenberg R Schachtex J | 2012 | Curt Top Med Chem2012，12，1： | 1 |
| 6 | Transcriptional regulations of vascular endothelial growth factor C by oxidative and thermal stress is mediated by lens epithelium-derived growth factor/p75显示文摘 | Cohen B Addadi Y Sapoznik S | 2009 | Neoplasia2009，11，9： | 1 |
| 7 | Peritoneal adhesion and angiogenesis in ovarian carcinoma are inversely regulated by hyaluronan: the role of gonadotropins显示文摘 | Tzuman Y C Sapoznik S Granot D | 2010 | Neoplasia2010，12，1： | 1 |
| 8 | CEACAM1 in malignantmelanoma:a diagnostic and therapeutic target显示文摘 | Sapoznik S Ortenberg R Schachter J | 2012 | Curr Top MedChem2012，12，1： | 1 |
| 9 | Energy aspects in osmotic processes显示文摘 | Semiat R Sapoznik J Hasson D | 2010 | Desalination and Water Treatment2010，15，： | 1 |
| 10 | CEACAM1inmalignant melanoma:a diagnostic and therapeutic target显示文摘 | Sapoznik S Ortenberg R Schachter J | 2012 | Curr Top Med Chem2012，12，1： | 1 |
| 11 | Gonadotropin-regulated lymphangiogenesis in ovarian cancer is mediated by LEDGFinduced expression of VEGF-C显示文摘 | Sapoznik S Cohen B Tzuman Y | 2009 | Cancer Res2009，69，24： | 1 |
| 12 | Transcriptional regulation of vascular endothelial growth factor C by oxidative and thermal stress is mediated by lens epithelium-derived growth factor/p75显示文摘 | Cohen B Addadi Y Sapoznik S | 2009 | Neoplasia2009，11，9： | 1 |
| 13 | CEACAM1 in malignant melanoma:a diagnostic and therapeutic target 显示文摘 | Sapoznik S Ortenberg R Schachter J | 2012 | CurrTopMedChem2012，12，1： | 1 |
| 14 | Gonadotropin-regulated lym- phangiogenesis in ovarian cancer is mediated by LEDGF-indueed expression of VEGF-C 显示文摘 | Sapoznik S Cohen B Tzuman Y | 2009 | Cancer Res2009，69，24： | 1 |
| 15 | Bioengineered Blood Vessels 显示文摘 | Niu G G Sapoznik E Soker S | 2014 | Expert Opinion Biological Therapy2014，14，4： | 1 |
| 16 | Novel anti-melanoma immunotherapies:disarming tumor escape mechanisms显示文摘 | Sapoznik S Hammer O Ortenberg R | 2012 | Clin Dev Immunol2012，2012，81： | 1 |
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