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| 1 | MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors显示文摘 | Liu Xi Sempere Lorenzo F Ouyang Haoxu Memoli Vincent A Andrew Angeline S Luo Yue Demidenko Eugene Korc Murray Shi Wei Preis Meir Dragnev Konstantin H Li Hua DiRenzo James Bak Mads Freemantle Sarah J Kauppinen Sakari Dmitrovsky Ethan | 2010 | Journal of Clinical Investigation2010，，4： | 3 |
| 2 | Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis显示文摘AIM:To identify and characterize drosophila mothers against decapentaplegic(SMAD)3-dependent changes in immune cell populations following infection with Helicobacter hepaticus(H.hepaticus).METHODS:SMAD3 -/-(n=19)and colitis-resistant SMAD3+/-(n=24)mice(8-10 wk of age)were in-fected with H.hepaticus and changes in immune cell populations[T lymphocytes,natural killer(NK)cells,T regulatory cells]were measured in the spleen and mesenteric lymph nodes(MsLNs)at 0 d,3 d,7 d and 28 d post-infection using flow cytometry.Genotypedependent changes in T lymphocytes and granzyme B+ cells were also assessed after 28 d in proximal colon tissue using immunohistochemistry.RESULTS:As previously observed,SMAD3 -/-,but not SMAD3+/-mice,developed colitis,peaking at 4 wk post-infection.No significant changes in T cell subsets were observed in the spleen or in the MsLNs between genotypes at any time point.However,CD4+and CD8+/ CD62Llo cells,an effector T lymphocyte population,as well as NK cells(NKp46/DX5+)were significantly higher in the MsLNs of SMAD3-/-mice at 7 d and 28 d post-infection.In the colon,a higher number of CD3+cells were present in SMAD3-/-compared to SMAD3+/-mice at baseline,which did not significantly change during infection.However,the number of granzyme B+cells,a marker of cytolytic lymphocytes,significantly increased in SMAD3-/-mice 28 d post-infection compared to both SMAD3+/-mice and to baseline values.This was consistent with more severe colitis development in these animals.CONCLUSION:Data suggest that defects in SMAD3 signaling increase susceptibility to H.hepaticus-induced colitis through aberrant activation and/or dysregulation of effector lymphocytes. | Sarah J McCaskey Elizabeth A Rondini Jonathan F Clinthorne Ingeborg M Langohr Elizabeth M Gardner Jenifer I Fenton | 2012 | World Journal of Gastroenterology2012，18，13： | 2 |
| 3 | Evidence for incorporation of free-floating cells as a mechanism of serosal healing 显示文摘 | Adam J F Sarah E H Talib AI-M | 2002 | J Cell Sci2002，115，7： | 1 |
| 4 | Stimulated erythro-poiesis with secondary iron loading lead to a decrease in hepcidin despite an increase in bone morphogenetic protein 6 expression 显示文摘 | DAVID M F SARAH J W DEEPAK D | 2012 | Br J Haematol2012，175，： | 1 |
| 5 | Human ge- netic variation and its contribution to complex traits 显示文摘 | Kelly A F Sarah S M Nicholas J S | 2009 | Nature Reviews Genetic2009，10，4： | 1 |
| 6 | A comprehensive review of the effects of rTMS on motor cortical excitability and inhibition显示文摘 | Fitzgerald P B Sarah F Daskalakis Z J | 2006 | Clinical Neurophysiology Official Journal of the International Federation of Clinical Neurophysiology2006，117，12： | 1 |
| 7 | A case study of cancer data set combinations for PCBs 显示文摘 | Sarah T V Susan F V Vincent J C | 2005 | Regulatory Toxicology and Pharmacology2005，46，： | 1 |
| 8 | Variants in the SP110 gene are associated with genetic susceptibility to tuberculosis in West Africa 显示文摘 | Kerrie T Sarah J Katherine F | 2006 | Proc Natl Acad Sci USA2006，103，10： | 1 |
| 9 | So what' s new in the field of plant cold acclimation 显示文摘 | Michael F Thomashow Sarah J | 2001 | Plant Physiol2001，125，： | 1 |
| 10 | Work - Family Facilitation: Expanding Theoretical Under- standing Through Qualitative exploration 显示文摘 | Jeffrey H Sarah A Jenet J Ashley F B Sacha L B | 2007 | Academy of Hu- man Resource Development2007，9，04： | 1 |
| 11 | Arabidopsis transcriptional activators CBF1,CBF2,and CBF3 have matching functional activities显示文摘 | SARAH J G SARAH G F MICHAEL F T | 2004 | Plant Molecular Biology2004，54，： | 1 |
| 12 | Arabidopsis thaliarut CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory ele- ment that stimulates transcription in response to low temperature and water deficit显示文摘 | Eric J Stockinger Sarah J Gilmour Michael F | 1997 | Proe Natl Acad Sci USA1997，94，： | 1 |
| 13 | Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42显示文摘AIM:To understand the interaction of human IQGAP1 and CDC42,especially the effects of phosphorylation and a cancer-associated mutation. METHODS:Recombinant CDC42 and a novel C-termi- nal fragment of IQGAP1 were expressed in,and puri- fied from,Escherichia coli.Site directed mutagenesis was used to create coding sequences for three phos- phomimicking variants(S1441E,S1443D and S1441E/ S1443D)and to recapitulate a cancer-associated mu- tation(M1231I).These variant proteins were also ex- pressed and purified.Protein-protein crosslinking using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide was used to investigate interactions between the C-terminal fragment and CDC42.These interactions were quanti- fied using surface plasmon resonance measurements.Molecular modelling was employed to make predictions about changes to the structure and flexibility of the protein which occur in the cancer-associated variant. RESULTS:The novel,C-terminal region of human IQGAP1 (residues 877-1558)is soluble following expression and purification.It is also capable of binding to CDC42,as judged by crosslinking experiments.Interaction appears to be strongest in the presence of added GTP.The three phosphomimicking mutants had different affini- ties for CDC42.S1441E had an approximately 200-fold reduction in affinity compared to wild type.This was caused largely by a dramatic reduction in the associa- tion rate constant.In contrast,both S1443D and the double variant S1441E/S1443D had similar affinities to the wild type.The cancer-associated variant,M1231I, also had a similar affinity to wild type.However,in the case of this variant,both the association and dis- sociation rate constants were reduced approximately 10-fold.Molecular modelling of the M1231I variant, based on the published crystal structure of part of the C-terminal region,revealed no gross structural changes compared to wild type(root mean square deviation of 0.564over 5556 equivalent atoms).However,pre- dictions of the flexibility of the polypeptide backbone suggested that some regions of the variant protein had greatly increased rigidity compared to wild type.One such region is a loop linking the proposed CDC42 bind- ing site with the helix containing the altered residue.It is suggested that this increase in rigidity is responsible for the observed changes in association and dissocia- tion rate constants. CONCLUSION:The consequences of introducing nega- tive charge at Ser-1441 or Ser-1443 in IQGAP1 are dif- ferent.The cancer-associated variant M1231I exerts its effects partly by rigidifying the protein. | Sarah F Elliott George Allen David J Timson | 2012 | World Journal of Biological Chemistry2012，3，3： | 1 |
| 14 | CD4+CD25+Foxp3+regulatoryT cells are increased whilst CD3+CD4-CD8-α,TCR+doublenegative T cells are decreased in the peripheral blood of patientswith multiple myeloma which correlates with disease burden显示文摘 | Sylvia F Sarah J Lee M | 2009 | British J of Haematology2009，144，5： | 1 |
| 15 | Is the 16S-23S internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria显示文摘 | SARAH L B VALERIE R F JEFFREY R J | 2001 | Mol Biol Evol2001，18，6： | 1 |
| 16 | Prediction of pre-eclampsia by abnormal uterine Doppler ultrasound and modification by aspirin 显示文摘 | SARAH J BOWER KEVIN F HARRINGTON LECTURER | 1996 | Br J Obstet Gynecol1996，103，： | 1 |
| 17 | Chemotherapy for schistoso- miasis in Ugandan fishermen: treatment can cause a rapid increase in interleukin-5 levels in plasma but decreased levels of eosinophilia and worm-specific immunoglobulin E 显示文摘 | Colin M F Sarah J Frances MJ | 2004 | Infect Imm2004，72，7： | 1 |
| 18 | 热文点评原文摘要显示文摘中文:背景患者入院后可从不当消毒的环境表面获得多药耐药菌和艰难梭菌。本文确定了3种强化的终末消毒(入住同一病房的两名患者之间的消毒)策略,对感染耐甲氧西林金黄色葡萄球菌(MRSA)、耐万古霉素肠球菌(VRE)、艰难梭菌(CD)和多重耐药不动杆菌的影响。方法本文在美国东南部的9家医院开展了一项务实的、集群-随机、交叉研究。凡曾有感染或定植目标细菌感染患者居住过的病房,患者出院后随机采取4种消毒策略中的一种方法进行终末消毒:对照(季胺盐类消毒剂消毒,但凡遇到CD采用含氯消毒剂);UV(季胺盐类+UV-C消毒,但凡遇到CD采用含氯消毒剂+UV-C);含氯消毒剂;含氯消毒剂+UV-C。凡入住目标病房的患者被列为暴露人群。这4种终末消毒方法分别在每家医院连续实施7个月的周期。本文随机设计这几种消毒策略在每家医院内的实施顺序(1:1:1:1)。主要产出的结果是,观察暴露患者中目标细菌的感染的发生或定植情况,以及ITT人群中暴露患者CD感染发生率。本研究ClinicalTrials.gov注册编号:NCT01579370。结果共有31226名患者暴露,其中21395(69%)符合标准,包括4916名对照组,5178名UV组,5438名含氯消毒剂组,以及5863名含氯消毒剂+UV组。在对照组中,22426个暴露日中有115名患者发生目标细菌的感染(51.3/10000暴露日)。在标准清洁策略的基础上增加UV消毒的暴露患者,其目标细菌感染的发生率明显较低(n=76;33.9/10000暴露日;RR:0.70,95%CI:0.50~0.988;P=0.036)。含氯消毒剂组(n=101;41.6/10000暴露日;RR:0.85,95%CI:0.69~1.04;P=0.116),或含氯消毒剂+UV组患者(n=131;45.6/10000暴露日;RR:0.91,95%CI:0.76~1.09;P=0.303)的目标细菌的感染率,其差异无统计学意义。同样,在含氯消毒剂的基础上增加UV消毒,暴露患者中CD感染率也没有发生改变((n=38vs36;30.4vs31.6/10000暴露日;RR:1.0,95%CI:0.57-1.75;P=0.997)。解释污染的医疗机构环境是获得病原微生物的重要来源;强化终末消毒可以降低这一风险。 | Deverick J Anderson Luke F Chen David J Weber Rebekah W Moehring Sarah S Lewis Patricia F Triplett Michael Blocker Paul Becherer J Conrad Schwab Lauren P Knelson Yuliya Lokhnygina William A Rutalo Hajimori Kanamori Marina F Gergen Daniel J Sexton | 2018 | 中华医院感染学杂志2018，28，12： | 1 |
| 19 | Phosphoryla- tion of immunity-related GTPase by a Toxoplasrna gondii secreted kinase promotes macrophage survival and viru- lence 显示文摘 | Sarah J F Micheal S B lldiko R D | 2010 | Cell Host Microbe2010，8，6： | 1 |
| 20 | Role of the Arabidopsis CBF transcriptional activators in cold acclimation 显示文摘 | Michael F Thomashow Sarah J Gilmour Eric J Stockinger l | 2001 | Physiol Plant2001，112，： | 1 |
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