| 8 | Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs显示文摘Decellularization of xenogeneic heart valves might lead to excellent regenerative implants,from which many patients could benefit.However,this material carries various xenogeneic epitopes and thus bears a considerable inherent immunological risk.Here,we investigated the regenerative and immunogenic potential of xenogeneic decellularized heart valve implants using pigs deficient for the galactosyltransferase gene(GGTA1-KO)as novel large animal model.Decellularized aortic and pulmonary heart valves obtained from sheep,wild-type pigs or GGTA1-KO pigs were implanted into GGTA1-KO pigs for 3,or 6 months,respectively.Explants were analyzed histologically,immunhistologically(CD3,CD21 and CD172a)and anti-aGal antibody serum titers were determined by ELISA.Xenogeneic sheep derived implants exhibited a strong immune reaction upon implantation into GGTA1-KO pigs,characterized by massive inflammatory cells infiltrates,presence of foreign body giant cells,a dramatic increase of anti-aGal antibody titers and ultimately destruction of the graft,whereas wild-type porcine grafts induced only a mild reaction in GGTA1-KO pigs.Allogeneic implants,wild-type/wild-type and GGTA1-KO/GGTA1-KO valves did not induce a measurable immune reaction.Thus,GGTA1-KO pigs developed a‘human-like’immune response toward decellularized xenogeneic implants showing that immunogenicity of xenogeneic implants is not sufficiently reduced by decellularization,which detracts from their regenerative potential. | Robert Ramm Tobias Goecke Peter Kohler Igor Tudorache Serghei Cebotari Anatol Ciubotaru Samir Sarikouch Klaus Hoffler Friederike Bothe Bjorn Petersen Axel Haverich Heiner Niemann Andres Hilfiker | 2021 | Regenerative Biomaterials2021，8，5： | 0 |
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