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您的检索式：作者名="Saskia Lippens"
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| 1 | Autophagy: for better or for worse显示文摘Autophagy 是降级的降级小径损坏了的 lysosomal 或过剩房间部件进基本 biomolecules,它然后回来被再循环进 cytosol。在这个方面, autophagy 在一个连续降级新生周期驾驶 biomolecules 的流动。Autophagy 通常被认为在应力或差的滋养的条件下面保护房间的支持幸存的机制。当前的研究清楚地证明 autophagy 在重要生物过程完成众多的功能。它在开发被含有,区别,天生、适应的免疫,变老并且房间死亡。另外,积累证据表明在 autophagy 和几人的疾病和肿瘤开发之间的有趣的连接。因此, autophagy 似乎是在细胞和有机体的生活和死亡的一个重要播放器。尽管有 autophagy 的增长知识, autophagic 机械通过调整这些多样的过程的机制完全没被理解。在这评论,我们给表明小径的 autophagic 的全面概述,它的角色一般来说细胞的过程和它的连接到房间死亡。另外,我们在场发信号到疾病的有缺点的 autophagic 的可能的贡献的简短概述。 | Ellen Wirawan Tom Vanden Berghe Saskia Lippens Patrizia Agostinis Peter Vandenabeele | 2012 | Cell Research2012，22，1： | 70 |
| 2 | Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy显示文摘In the replacement of genetic probes,there is increasing interest in labeling living cells with high-quality extrinsic labels,which avoid over-expression artifacts and are available in a wide spectral range.This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells.Here,we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique.We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation.We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses,allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells.We demonstrate high-quality time-lapse imaging with confocal,total internal reflection fluorescence(TIRF),and Airyscan superresolution microscopy.As the entire procedure is readily compatible with fluorescence(super resolution)microscopy,photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging. | Jing Liu Ranhua Xiong Toon Brans Saskia Lippens Eef Parthoens Francesca Cella Zanacchi Raffaella Magrassi Santosh K.Singh Sreekumar Kurungot Sabine Szunerits Hannelore Bové Marcel Ameloot Juan C.Fraire Eline Teirlinck Sangram Keshari Samal Riet De Rycke Gaëlle Houthaeve Stefaan C.De Smedt Rabah Boukherroub Kevin Braeckmans | 2018 | Light(Science & Applications)2018，7，1： | 1 |
| 3 | Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation显示文摘Fluorescence microscopy is the method of choice for studying intracellular dynamics.However,its success depends on the.availability of specific and stable markers.A prominent example of markers that are rapidly gaining interest are nanobodies(Nbs.-15 kDa),which can be functionalized with bright and photostable organic fluorophores.Due to their relatively small size and high specificity,Nbs offer great potential for high-quality long-term subcellular imaging,but suffer from the fact that they cannot spontaneously cross the plasma membrane of live cells.We have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their viability.Being a laser-based technology,it is readily compatible with light microscopy and the typical cell recipients used for that.Spurred by these promising initial results,we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living cells.We illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm,actin-bundling protein Fascin,and the histone H2A/H2B heterodimers.With an efficiency of more than 80%labeled cells and minimal toxicity(-2%),photoporation proved to be an excellent intracellular delivery method for Nbs.Time-lapse microscopy revealed that cell division rate and migration remained unaffected,confirming excellent cell viability and functionality.We conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells,laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy. | Jing Liu Tim Hebbrecht Toon Brans Eef Parthoens Saskia Lippens Chengnan Li Herlinde De Keersmaecker Winnok H.De Vos Stefaan C.De Smedt Rabah Boukherroub Jan Gettemans Ranhua Xiong Kevin Braeckmans | 2020 | Nano Research2020，13，2： | 0 |
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