|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Dynamic window constrained-scheduling of real-time streams in media servers显示文摘 | West R Zhang Yu-ting Sehwan K | 2004 | IEEE Transactions on Computers2004，53，6： | 1 |
| 2 | Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor显示文摘AIM To investigate a suitable long-term culture system and optimal cryopreservation of intestinal organoid to improve organoid-based therapy by acquiring large numbers of cells.METHODS Crypts were isolated from jejunum of C57BL/6 mouse. Two hundred crypts were cultured in organoid medium with either epidermal growth factor/Noggin/R-spondin1(ENR) or ENR/CHIR 99021/VPA(ENR-CV). F o rsubculture, organoids cultured on day 7 were passaged using enzyme-free cell dissociation buffer (STEMCELL Technologies). The passage was performed once per week until indicated passage. For cryopreservation, undissociated and dissociated organoids were resuspended in freezing medium with or without Rho kinase inhibitor subjected to different treatment times. The characteristics of intestinal organoids upon extended passage and freeze-thaw were analyzed using Ed U staining, methyl thiazolyl tetrazolium assay, q PCR and time-lapse live cell imaging.RESULTS We established a three-dimensional culture system for murine small intestinal organoids using ENR and ENR-CV media. Both conditions yielded organoids with a crypt-villus architecture exhibiting Lgr5^+ cells and differentiated intestinal epithelial cells as shown by morphological and biochemical analysis. However, during extended passage (more than 3 mo), a comparative analysis revealed that continuous passaging under ENR-CV conditions, but not ENR conditions induced phenotypic changes as observed by morphological transition, reduced numbers of Lgr5^+ cells and inconsistent expression of markers for differentiated intestinal epithelial cell types. We also found that recovery of long-term cryopreserved organoids was significantly affected by the organoid state, i.e., whether dissociation was applied, and the timing of treatment with the Rho-kinase inhibitor Y-27632. Furthermore, the retention of typical morphological characteristics of intestinal organoids such as the crypt-villus structure from freeze-thawed cells was observed by live cell imaging.CONCLUSION The maintenance of the characteristics of intestinal organoids upon extended passage is mediated by ENR condition, but not ENR-CV condition. Identified long-term cryopreservation may contribute to the establishment of standardized cryopreservation protocols for intestinal organoids for use in clinical applications. | Sung-Hoon Han Sehwan Shim Min-Jung Kim Hye-Yun Shin Won-Suk Jang Sun-Joo Lee Young-Woo Jin Seung-Sook Lee Seung Bum Lee Sunhoo Park | 2017 | World Journal of Gastroenterology2017，23，6： | 1 |
| 3 | Eccentric exercise-induced injury to rat skeletal muscle显示文摘 | Armstrong R B Ogilvie R W Sehwane J A | 1983 | JApplPhysiol1983，54，： | 1 |
| 4 | Flow boiling heat transfer characteristics of R123 and R134a in a micro-channel显示文摘 | Sehwan In Sangkwon Jeong | 2009 | International Journal of Multiphase Flow2009，，35： | 1 |
| 5 | Eccentric exercise-induced injury to rat skeletal muscle显示文摘 | R B Armstrong R W Ogilvie J A Sehwane | 1983 | Appl Physiol1983，54，1： | 1 |
| 6 | Analysis and Minimization of Power-transmission Loss in Locally Daisy-chained Systems by Local Energy Buffering显示文摘 | SEHWAN K CHOU P H | 2013 | ACM Transactions on Design Automation of Electronic Systems2013，18，3： | 1 |
| 7 | Design and Performan- ce Analysis of Supercapacitor Charging Circuits for Wireless Sen- sor Nodes 显示文摘 | SEHWAN K KEUN-SIK N CHOU P H | 2011 | Emerging and Selected Topics in Circuits and Sys- tems2011，1，3： | 1 |
| 8 | Mitigating effects of hUCB-MSCs on the hematopoietic syndrome resulting from total body irradiation显示文摘 | Sehwan Shim Seung Bum Lee Jong-geol Lee Won-Suk Jang Sun-Joo Lee Sunhoo Park Seung-Sook Lee | 2013 | Experimental Hematology2013，，： | 1 |
| 9 | Investigation on Liquid Helium Pressurization Process Using a Heater in a Liquid Propellant Rocket显示文摘 | Sehwan In Sangkwon jeong Hongseong Kim | 2004 | Cryogenics2004，44，： | 1 |
| 10 | E2F1 Expression is Related with the Poor Survival of Lymph Node-positive Breast Cancer Patients Treated with Fluorouracil, Doxorubicin and Cyclophosphamide显示文摘 | Sehwan Han Kyeongmee Park Byung-Noe Bae Ki Hwan Kim Hong-Joo Kim Young-Duck Kim Hong-Yong Kim | 2003 | Breast Cancer Research and Treatment2003，，1： | 1 |
| 11 | Investigation on liquid helium pressurization process using a heater in a liquid propellant rocket 显示文摘 | Sehwan In Sangkwon Jeong Hongseong Kim | 2004 | Cryogenics2004，44，： | 1 |
| 12 | Temperature effect on the vibratiowbased electrostatic energy har- vester显示文摘 | SEHWAN K KUKJIN C SUNGIL B | 2011 | IEEE Tencon2011，13，3： | 1 |
| 13 | Dielectric properties of tissues and biological materials: A critical review 显示文摘 | Foster K Sehwan H | 1989 | Crit Rev Biomed Eng1989，17，1： | 1 |
| 14 | Real-time remote monitoring: the DuraMote platform and experiments towards future, advanced, large-scale SCADA systems显示文摘 | Masanobu Shinozuka Konstantinos G. Papakonstantinou Marco Torbol Sehwan Kim | 2015 | Structure and Infrastructure Engineering2015，，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员