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| 1 | Bushen Qiangjin capsule(补肾强筋胶囊) inhibits the Wnt/β-catenin pathway to ameliorate papain-induced knee osteoarthritis in rats显示文摘OBJECTIVE:To evaluate the molecular mechanism underlying the beneficial effect of Bushen Qiangjin capsule(补肾强筋胶囊,BSQJ),a Traditional Chinese Medicine,on knee osteoarthritis(KOA).METHODS:In the present study,32 female Sprague-Dawley rats were randomly divided into four groups:control,KOA,high-dose BSQJ(H-BSQJ),and low-dose BSQJ(L-BSQJ).After successfully establishing the KOA model by intra-articular injection of papain,H-BSQJ and L-BSQJ groups were intragastrically administered 0.243 and 0.122 g/kg BSQJ,respectively,daily for 6 weeks.At the end of the experiment,knee articular cartilage tissues of rats were collected for evaluation by hematoxylin and eosin staining,Safranin O-Fast Green staining,and terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling assay.Serum interleukin-1βand tumor necrosis factor-αlevels of rats were detected with an enzyme-linked immunosorbent assay method.Gene expression of Wnt-4,β-catenin,Frizzled-2,glycogen synthase kinase-3β(GSK-3β),cysteinyl aspartate-specific proteinases 3 and 9(caspases 3 and 9),collagen typeⅡalpha 1(Col2 a1),and matrix metalloproteinases 1 and 13(MMP-1 and MMP-3)of rat knee articular cartilage was quantified by reverse transcription-quantitative polymerase chain reaction analysis.Wnt-4,β-catenin,Frizzled-2,GSK-3β,cleaved caspase-3,and cleaved caspase-9 protein expression in rat knee articular cartilage was determined by western blot analysis.RESULTS:BSQJ obviously reduced pathological damage and matrix degradation of articular cartilage in KOA rats.Compared with the KOA group,H-BSQJ rats exhibited downregulated m RNA and protein expression of Wnt-4,β-catenin,Frizzled-2,and caspase-3,as well as upregulated m RNA and protein expression of GSK-3β.In addition,H-BSQJ significantly increased m RNA expression of Col2 a1 and decreased m RNA expression of MMP-1 and MMP-13.CONCLUSION:BSQJ exerted a beneficial effect on KOA by a mechanism involving downregulation of the Wnt/β-catenin pathway,which inhibited both cartilage extracellular matrix degradation and chondrocyte apoptosis to ameliorate KOA in rats. | YAO Nan CHEN Guocai LU Yanyan XU Xuemeng ZHAO Chuanxi HUANG Xuejun LIU Wengang PENG Sha WU Huai | 2021 | Journal of Traditional Chinese Medicine2021，41，6： | 5 |
| 2 | Nonlinear Finite Element Analysis of Barge Collision with a Single Bridge Pier显示文摘 | Sha Yanyan Hao Hong | 2012 | Engineering Structures2012，41，： | 1 |
| 3 | Application of soybean shoot-cutting in SMV-resistance genetic analysis显示文摘 | Haifeng Chen Zhonglu Yang Qiao Wan Yanyan Yang Shuilian Chen Chanjuan Zhang Limiao Chen Songli Yuan Dezhen Qiu Zhihui Sha Xin'an zhou | 2016 | Oil Crop Science2016，1，3： | 0 |
| 4 | Numerical study on the effect of in-situ stress on smoothwall blasting in deep tunnelling显示文摘In the present study,a numerical model is first calibrated against the crack networks and pressure attenuation data in laboratory blasting test.Then,based on the calibrated numerical model,two-hole plane models are developed and used to perform a series of sim-ulations of smoothwall blasting in deep tunnelling subjected to in-situ stress.The evolutions of rock fracture and excavation damage zone in the roof/floor and sidewalls under different far-field hydrostatic pressure and anisotropic in-situ stress conditions are numerically investigated.The findings in numerical modelling are also analytically interpreted with the stress distributions around the designed tunnel perimeter and perimeter borehole.The numerical and analytical results show that the variations of rock cracking and excavation dam-aged zone induced by smoothwall blasting with in-situ stress are mainly attributed to the high tangential compressive stress concentration around the remaining rock after inner primary blasts and the tensile stress acting on the wall of perimeter hole,which control the crack propagation and initiation respectively.At last,the implications of findings for practical smoothwall blasting in deep tunnelling are discussed. | Xudong Li Kewei Liu Jiacai Yang Yanyan Sha Ruitao Song | 2023 | Underground Space2023，，4： | 0 |
| 5 | Haplotype variation and KASP markers for SiPSY1-A key gene controlling yellow kernel pigmentation in foxtail millet显示文摘Carotenoid biosynthesis and accumulation are important in determining nutritional and commercial value of crop products.Yellow pigmentation of mature kernels caused by carotenoids is considered a vital quality trait in foxtail millet,an ancient and widely cultivated cereal crop across the world.Genomic regions associated with yellow pigment content(YPC),lutein and zeaxanthin in foxtail millet grains were identified by genome-wide association analysis(GWAS),and SiPSY1(Phytoene synthase 1 which regulates formation of the 40-carbon backbone of carotenoids)was confirmed as the main contributor to all three components by knockout and overexpression analysis.SiPSY1 was expressed in seedlings,leaves,panicles,and mature seeds,and was subcellularly localized to chloroplasts.Transcription of SiPSY1 in 15 DAP immature grains was responsible for YPC in mature seeds.Selection of SiPSY1 combined with increased YPC in mature grains during domestication of foxtail millet was confirmed.Haplotype analysis suggested that expression level of SiPSY1 could be a selection target for future breeding programs,and a KASP marker was developed for selection of favorable SiPSY1 alleles in breeding.The results of this work will benefit nutritional and commercial improvement of foxtail millet varieties,as well as other cereal crops. | Rongjun Zuo Yanyan Zhang Yanbing Yang Chunfang Wang Hui Zhi Linlin Zhang Sha Tang Yanan Guan Shunguo Li Ruhong Cheng Zhonglin Shang Guanqing Jia Xianmin Diao | 2023 | The Crop Journal2023，11，6： | 0 |
| 6 | De novo creation of popcorn-like fragrant foxtail millet显示文摘Aroma plays a crucial role in the sensory appeal and enjoyment of food,and it significantly impacts the grain quality of cereals,thereby influencing the value and consumption of agricultural products.For example,aromatic rice varieties like Basmati and Jasmine are renowned for their distinct aroma and command almost double the price of regular rice in the market. | Yanyan Zhang Qiang He Shihui Zhang Xinyu Man Yi Sui Guanqing Jia Sha Tang Hui Zhi Chuanyin Wu Xianmin Diao | 2023 | Journal of Integrative Plant Biology2023，65，11： | 0 |
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