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| 1 | Constitutive activation of Stat3 signaling pathway in human colorectal carcinoma显示文摘AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-XL in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-xu proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-XL were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of pStat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation. | Xiang-TaoMa ShanWang Ying-JiangYe Ru-YuDu Zhi-RongCui MaSomsouk | 2004 | World Journal of Gastroenterology2004，10，11： | 68 |
| 2 | Searching Databases with Keywords显示文摘Traditionally, SQL query language is used to search the data in databases. However, it is inappropriate for end-users, since it is complex and hard to learn. It is the need of end-user, searching in databases with keywords, like in web search engines. This paper presents a survey of work on keyword search in databases. It also includes a brief introduction to the SEEKER system which has been developed. | ShanWang Kun-LongZhang | 2005 | Journal of Computer Science & Technology2005，20，1： | 16 |
| 3 | Increased expression of mitogen-activated protein kinase and its upstream regulating signal in human gastric cancer显示文摘AIM: To investigate the expression of mitogen-activated protein kinases (MAPKs) and its upstream protein kinase in human gastric cancer and to evaluate the relationship between protein levels and clinicopathological parameters.METHODS: Westem blot was used to measure the expression of extracellular signal-regulated kinase (ERK)-1, ERK-2, ERK-3,p38 and mitogen or ERK activated protein kinaseMEK-1proteins in surgically resected gastric carcinoma, adjacent normal mucosa and metastatic lymph nodes from 42 patients.Immunohistochemistry was employed for their localization.RESULTS: Compared with normal tissues, the protein levels of ERK-1 (integral optical density value 159 526±65 760 vs122 807±65 515, P = 0.001), ERK-2 (168 471±95 051 vs120 469±72 874, P<0.001), ERK-3 (118 651±71 513 vs70 934±68 058, P<0.001), P38 (104 776±51 650 vs82 930±40 392, P= 0.048) and MEK-1(116 486±45 725 vs101 434±49 387, P = 0.027) were increased in gastric cancer tissues. Overexpression of ERK-3 was correlated to TNM staging [average ratio of integral optic density (IOD)tumor:IODnormal in TNM Ⅰ, Ⅱ, Ⅲ, Ⅳ tumors was 1.43±0.34,5.08±3.74, 4.99±1.08, 1.44±1.02, n = 42, P = 0.023] and serosa invasion (4.31±4.34 vs 2.00±2.03, P = 0.037). In poorly differentiated cancers (n = 33), the protein levels of ERK-1 and ERK-2 in stage Ⅲ and Ⅳ tumors were higher than those in stage Ⅰ and Ⅱ tumors (2.64±3.01 vs 1.01±0.33,P = 0.022; 2.05±1.54 vs 1.24±0.40, P = 0.030). Gastric cancer tissues with either lymph node involvement (2.49±2.91vs 1.03±0.36, P= 0.023; 1.98±1.49 vs 1.24±0.44, P= 0.036)or serosa invasion (2.39±2.82 vs 1.01±0.35, P = 0.022;1.95±1.44 vs 1.14±0.36, P = 0.015) expressed higher protein levels of ERK-1 and ERK-2. In Borrmann Ⅱ tumors,expression of ERK-2 and ERK-3 was increased compared with Borrmann Ⅲ tumors (2.57±1.86 vs 1.23±0.60, P = 0.022;5.50±5.05 vs 1.83±1.21, P = 0.014). Borrmann Ⅳ tumors expressed higher p38 protein levels. No statistically significant difference in expression of MAPKs was found when stratified to tumor size or histological grade (P>0.05).Protein levels of ERK-2, ERK-3 and MEK-1 in metastatic lymph nodes were 2-7 folds higher than those in adjacent normal mucosa. The immunohistochemistry demonstrated that ERK-1, ERK-2, ERK-3, p38 and MEK-1 proteins were mainly localized in cytoplasm. The expression of MEK-1 in gastric cancer cells metastasized to lymph nodes was higher than that of the primary site.CONCLUSION: MAPKs, particularly ERK subclass are overexpressed in the majority of gastric cancers.Overexpression of ERKs is correlated to TNM staging, serosa invasion, and lymph node involvement. The overexpression of p38 most likely plays a prominent role in certain morphological subtypes of gastric cancers. MEK-1 is also overexpressed in gastric cancer, particularly in metastatic lymph nodes. Upregulation of MAPK signal transduction pathways may play an important role in tumorigenesis and metastatic potential of gastric cancer. | BinLiang ShanWang Xue-GuangZhu Yong-XiangYu Zhi-RongCui You-ZhiYu | 2005 | World Journal of Gastroenterology2005，11，5： | 15 |
| 4 | In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells显示文摘The prevention of recurrence of colon cancer (CC)after operation is very important for improvement of theprognosis of CC patients, especially those with micro-metastasis. The generation of fused cells between dendriticcells (DCs) and tumor cells maybe an effective approach fortumor antigen presentation in immunotherapy. In this study,we fused human colon caner SW480 cells and humanperipheral blood - derived DCs to induce an antitumor activityagainst human CC. | FengXu Ying-JiangYe ShanWang | 2004 | World Journal of Gastroenterology2004，10，8： | 5 |
| 5 | Incremental Maintenance of Quotient Cube Based on Galois Lattice显示文摘Data cube computation is a well-known expensive operation and has been studied extensively. It is often not feasible to compute a complete data cube due to the huge storage requirement. Recently proposed quotient cube addressed this fundamental issue through a partitioning method that groups cube cells into equivalent partitions. The effectiveness and efficiency of the quotient cube for cube compression and computation have been proved. However, as changes are made to the data sources, to maintain such a quotient cube is non-trivial since the equivalent classes in it must be split or merged. In this paper, incremental algorithms are designed to update existing quotient cube efficiently based on Galois lattice. Performance study shows that these algorithms are efficient and scalable for large databases. | Cui-PingLi Kum-HoeTung ShanWang | 2004 | Journal of Computer Science & Technology2004，19，3： | 2 |
| 6 | Semi-Closed Cube: An Effective Approach to Trading Off Data Cube Size and Query Response Time显示文摘The results of data cube will occupy huge amount of disk space when the base table is of a large number of attributes. A new type of data cube, compact data cube like condensed cube and quotient cube, was proposed to solve the problem. It compresses data cube dramatically. However, its query cost is so high that it cannot be used in most applications. This paper introduces the semi-closed cube to reduce the size of data cube and achieve almost the same query response time as the data cube does. Semi-closed cube is a generalization of condensed cube and quotient cube and is constructed from a quotient cube. When the query cost of quotient cube is higher than a given threshold, semi-closed cube selects some views and picks a fellow for each of them. All the tuples of those views are materialized except those closed by their fellows. To find a tuple of those views, users only need to scan the view and its fellow. Thus, their query performance is improved. Experiments were conducted using a real-world data set. The results show that semi-closed cube is an effective approach of data cube. | Sheng-EnLi ShanWang | 2005 | Journal of Computer Science & Technology2005，20，3： | 2 |
| 7 | Clinical study on the correlation between metabolic syndrome and colorectal carcinoma显示文摘 | ZhanlongShen ShanWang YingjiangYe MujunYin XiaodongYang KeweiJiang YanLiu | 2010 | ANZ Journal of Surgery2010，，5： | 1 |
| 8 | 基于“哥雷斯”格的“综合”数据方体增量维护显示文摘众所周知,数据方体预计算可以在一定程度上提高OLAP操作的效率。但同时,数据方体预计算又是一件非常耗费时间的操作,并且计算的结果集非常大,需要很大的存储空间。Lakshmanan等2002年提出了一种“综合”数据方体。它在等价划分理论的基础上通过抽取数据方体的语义信息,将数据方体进行了有效的缩减,从而节约了该数据方体的计算时间和存储空间。 | Cui-PinqLi Kum-HoeTung ShanWang | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 9 | Analysis of amino acid enantiomers in ambient aerosols:Effects and removal of coexistent aerosol matrix显示文摘Amino acids(AAs)including D-and L-enantiomers are a group of organic nitrogen species in ambient aerosol.Due to the low abundances of AAs(level of ng/m^(3))and the matrix effects by coexistent components,it is challenging to quantify AA enantiomers in ambient aerosols especially under pollution conditions.In this study,we present an optimized method for analyzing AA enantiomers in atmospheric aerosol samples including a pretreatment process and the detection by high performance liquid chromatography coupled to a fuorescence detector(HPLC-FLD).Matrix effects caused by coexistent chemicals on AA enantiomers analysis in ambient aerosol samples were investigated especially for those collected in pollution episodes.The results revealed that the determination of AA enantiomers is significantly affected by the coexistent organic carbon(as a proxy of organic matter)and water-soluble ion of NH_(4)^(+).To remove the matrix effects,we applied a pretreatment using the solid phase extraction column coupled with alkaline adjustment to sample extract.After pretreatment,18 AAs including 6 pairs of D-and L-enantiomers(i.e.,leucine,isoleucine,valine,alanine,serine,and aspartic acid)can be successfully separated and quantified in aerosol samples by HPLC-FLD.The recoveries are in the range of 67%-106%.This method was successfully applied to the urban aerosol samples from pollution and non-pollution periods for AA enantiomers determination.We suggest that the concentrations of D-AAs and the ratio of D-AA/L-AA are indicative of the contribution of bacterial sources and the infuence of biomass burning. | Ying Li Xiaoying Li Libin Wu Luhan Shi ShanWang Pingqing Fu Yingyi Zhang Senchao Lai | 2024 | Journal of Environmental Sciences2024，，3： | 0 |
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