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| 1 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 2 | Nitric oxide-generating compound and bio-clickable peptide mimic for synergistically tailoring surface anti-thrombogenic and anti-microbial dual-functions显示文摘Application of extracorporeal circuits and indwelling medical devices has saved many lives.However,it is accompanied with two major complications:thrombosis and infection.To address this issue,we apply therapeutic nitric oxide gas(NO)and antibacterial peptide for synergistically tailoring such devices for surface anti-thrombogenic and antifouling dual functions.Such functional surface is realized by stepwise conjugation of NO-generating compound of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid(DOTA)chelated copper ions(Cu-DOTA)and dibenzylcyclooctyne-(DBCO-)modified antimicrobial peptide based on carbodiimide and click chemistry respectively.The integration of peptide and Cu-DOTA grants the modified surface the ability to not only efficiently inhibit bacterial growth,but also catalytically generate NO from endogenous s-nitrosothiols(RSNO)to reduce adhesion and activation of platelets,preventing the formation of thrombus.We envision that the stepwise synergistic modification strategy by using anticoagulant NO and antibacterial peptide would facilitate the surface multifunctional engineering of extracorporeal circuits and indwelling medical devices,with reduced clinical complications associated with thrombosis and infection. | Han Yu Shaoxing Yu Hua Qiu Peng Gao Yingzhong Chen Xin Zhao Qiufen Tu Minggang Zhou Lin Cai Nan Huang Kaiqin Xiong Zhilu Yang | 2021 | Bioactive Materials2021，6，6： | 3 |
| 3 | The influence of manufacturing parameters and adding support layer on the properties of Zirfon separators显示文摘polysulfone 和氧化锆包括的合成隔板被阶段倒置降水技术准备。在它的性质上生产参数的影响被调查,并且结果证明生产参数显著地影响离子的抵抗和最大的毛孔尺寸。有支持层的一个修改合成隔板被准备提高隔板的张力的力量。由增加支持层,隔板的张力的力量从 1.85MPa 增加到 13.66 MPa。以便评估合成隔板的实际适用性,一个小规模的工业电解实验被进行调查房间电压,煤气的纯净和隔板稳定性的变化。结果证明修改合成隔板有更小的房间电压和更高的 H < 潜水艇 class= “ a-plus-plus ” > 2 纯净比石棉隔板,并且为工业氢正在答应材料生产。 | Li XU Yue YU Wei LI Yan YOU Wei XU Shaoxing ZHANG | 2014 | Frontiers of Chemical Science and Engineering2014，8，3： | 1 |
| 4 | Observation and nursing in rescuing organic phosphorus poisoning by active carbon显示文摘CLINICALINFOMATIONANDMETHODSThe42organicphosphoruspoisoned,16maleand26female,aged18~61,haveanaverageof34.6years.Thetoxicantar... | JIN Xiang cheng,CHEN Hao ming,HUO Xing na, ZHENG Mei,YU Mei fang,TANG Xin gong,WANG Tao Blood Purification Centre of Shaoxing Peoples Hospital,Zhejiang 312000,China | 1999 | Chinese Journal of Biomedical Engineering(English Edition)1999，8，1： | 0 |
| 5 | Long-term in vivo chimeric cells tracking in non-human primate显示文摘Non-human primates(NHPs)are increasingly used in preclinical trials to test the safety and efficacy of biotech-nology therapies.Nonetheless,given the ethical issues and costs associated with this model,it would be highly advantageous to use NHP cellular models in clinical studies.However,developing and maintaining the naive state of primate pluripotent stem cells(PSCs)remains difficult as does in vivo detection of PSCs,thus limiting biotech-nology application in the cynomolgus monkey.Here,we report a chemically defined,xeno-free culture system for culturing and deriving monkey PSCs in vitro.The cells display global gene expression and genome-wide hypometh-ylation patterns distinct from monkey-primed cells.We also found expression of signaling pathways components that may increase the potential for chimera formation.Crucially for biomedical applications,we were also able to integrate bioluminescent reporter genes into monkey PsCs and track them in chimeric embryos in vivo and in vitro.The engineered cells retained embryonic and extra-embryonic developmental potential.Meanwhile,we generated a chimeric monkey carrying bioluminescent cells,which were able to track chimeric cells for more than 2 years in living animals.Our study could have broad utility in primate stem cell engineering and in utilizing chimeric monkey models forclinical studies. | Junmo Wu Yu Kang Xiang Luo Shaoxing Dai Yuxi Shi Zhuoyao Li Zengli Tang Zhenzhen Chen Ran Zhu Pengpeng Yang Zifan Li Hong Wang Xinglong Chen Ziyi Zhao Weizhi ji Yuyu Niu | 2024 | Protein & Cell2024，15，3： | 0 |
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