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| 1 | Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process显示文摘Ribozyme probe based on molecular beacon (MBR) for monitoring enzymatic cleavage process in real time is designed and studied. The approach relies on ri-bozyme substrates modified at the two arms, with a fluores-cent moiety attached to the end of one arm and a non-fluorescent quenching moiety attached to the end of the other arm. MBR is employed to directly convert the cleavage information into fluorescence signal in real time. Compared with traditional approach, this method provides a no-radiolabeling, sensitive and effective way to research on the ribozyme activity, enzymatic dynamic process and ri-bozyme function during gene therapy. The activity of the ribozyme against hepatitis C virus RNA (HCV-RNA) is stud-ied based on this assay. | MENG Xiangxian, WANG Kemin, TAN Weihong, LI Jun, TANG Zhiwen, GUO Qiuping, HUANG Shasheng & LI Du State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engi-neering, Hunan University, Changsha 410082, China Correspondence should be addressed to Wang Kemin (e-mail: kmwang @hnu.net.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 3 |
| 2 | The amplified fluorescence quenching of heteroatomic conjugated polymers based on the “molecular wire”effects显示文摘Tremendous interest has been evoked in the utility of the fluorescent conjugated polymers towards the creation of ultra-sensitive systems based on the molecular wire effects which can amplify the transduction events. Here, a series of new polymers pyridine substitute poly (phen- ylene ethynylene)s are synthesized by Sonogashira-Hagihara coupling reaction. The impact of structure modification on the physical properties and spectral characteristics of poly-mers has been investigated. The quenching studies are car-ried out and exhibit that recognition site (pyridyle group) highly influences the quenching efficiency. The results show that polymer PI with pyridyl group entrapped in the conju-gated backbone has the specific and sensitive response to Pd(Ⅱ). The fluorescence quenching of PⅠby Pd(Ⅱ) is about 65 times greater than that of model monomer. | HUANG Hongmei WANG Kemin XIAO Yi ZHAI Qiuge AN Delie HUANG Shasheng LI Du | 2003 | Chinese Science Bulletin2003，48，18： | 2 |
| 3 | β-cyclodextrin modified silica nanochannel membrane for chiral separation显示文摘 | Yue Liu Ping Li Li Xie Dingyan Fan Shasheng Huang | 2014 | Journal of Membrane Science2014，，： | 1 |
| 4 | Cellular distribution of ions in salt - stressed cells of Populus euphratica and P, tomentosa 显示文摘 | Chen Shaoliang Fritz E Wang Shasheng | 2000 | Forestry Studies in China2000，2，2： | 1 |
| 5 | Fluorescent Biological Label for Recognizing Human Ovarian Tumor Cells Based on Fluorescent Nanoparticles显示文摘 | Shasheng Huang Ruina Li Yongxia Qu Jian Shen Jia Liu | 2009 | Journal of Fluorescence2009，，6： | 1 |
| 6 | Photosynthetic reponse of Populus euphratica to salt stress 显示文摘 | Ma Huaneheng Fung Lindsay Wang Shasheng | 1997 | Forest Ecology and Management1997，93，： | 1 |
| 7 | Effects of NaCl on shoot growth,transpiration,ion compartmentation,and transport in regenerated plants of Populus euphratica and Populus tomentosa显示文摘 | CHEN Shaoliang LI Jinke WANG Shasheng | | 0，，06： | 1 |
| 8 | Real time monitoring of nucleic acids ligation based on molecular beacon显示文摘A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluo-rescence signal quickly and quantitatively. This method pro-vides effective and original approach to researching the dy-namic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.3×10-4 U/mL to 0.23 U/mL. It is rapid and sensitive to detect 2.8×10-5 U T4 DNA ligase in 10 min. | Zhiwen Tang Kemin Wang Weihong Tan Jun Li Lingfeng Liu Qiuping Guo Xiangxian Meng Shasheng Huang Du Li Weifang Luo | 2003 | Chinese Science Bulletin2003，48，12： | 1 |
| 9 | Sodium and chloride distribution in roots and transport in three poplar genotypes under increasing NaCl stress显示文摘 | Shaoliang Chen Jinke Li Eberhard Fritz Shasheng Wang Aloys Hüttermann | 2002 | Forest Ecology and Management2002，，1： | 1 |
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