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| 1 | CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement显示文摘The central dogma(CD)of molecular biology is the transfer of genetic information from DNA to RNA to protein.Major CD processes governing genetic flow include the cell cycle,DNA replication,chromosome packaging,epigenetic changes,transcription,posttranscriptional alterations,translation,and posttranslational modifications.The CD processes are tightly regulated in plants to maintain genetic integrity throughout the life cycle and to pass genetic materials to next generation.Engineering of various CD processes involved in gene regulation will accelerate crop improvement to feed the growing world population.CRISPR technology enables programmable editing of CD processes to alter DNA,RNA,or protein,which would have been impossible in the past.Here,an overview of recent advancements in CRISPR tool development and CRISPR-based CD modulations that expedite basic and applied plant research is provided.Furthermore,CRISPR applications in major thriving areas of research,such as gene discovery(allele mining and cryptic gene activation),introgression(de novo domestication and haploid induction),and application of desired traits beneficial to farmers or consumers(biotic/abiotic stress-resilient crops,plant cell factories,and delayed senescence),are described.Finally,the global regulatory policies,challenges,and prospects for CRISPR-mediated crop improvement are discussed. | Dibyajyoti Pramanik Rahul Mahadev Shelake Mi Jung Kim Jae-Yean Kim | 2021 | Molecular Plant2021，14，1： | 2 |
| 2 | Lung endothelial cell apoptosis during ischemic acute kidney injury 显示文摘 | White LE Cui Y Shelak CM | 2012 | Shock2012，38，3： | 1 |
| 3 | FPGA based packet splitter implementation using mixed design flow显示文摘 | Shinde S A Shelake V G Kamat R K | 2008 | Electronics and Electrical Engineering2008，8，88： | 1 |
| 4 | Structural,optical and electrical studies on pulse electrodeposited CdIn2S4 thin films显示文摘 | Kokate A V Asabe M R Shelake S B | | 0，，： | 1 |
| 5 | FPGA based packet splitter implementation using mixed design flow显示文摘 | SHINDE S A SHELAKE V G KAMAT R K | 2008 | Electronics and Electrical Engineering2008，88，8： | 1 |
| 6 | Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing:Targets,tools,challenges,and perspectives显示文摘Prolonged periods of drought triggered by climate change hamper plant growth and cause substantial agricultural yield losses every year.In addition to drought,salinity is one of the major abiotic stresses that severely affect crop health and agricultural production.Plant responses to drought and salinity involve multiple processes that operate in a spatiotemporal manner,such as stress sensing,perception,epigenetic modifications,transcription,post-transcriptional processing,translation,and post-translational changes.Consequently,drought and salinity stress tolerance are polygenic traits influenced by genomeenvironment interactions.One of the ideal solutions to these challenges is the development of highyielding crop varieties with enhanced stress tolerance,together with improved agricultural practices.Recently,genome-editing technologies,especially clustered regularly interspaced short palindromic repeats(CRISPR)tools,have been effectively applied to elucidate how plants deal with drought and saline environments.In this work,we aim to portray that the combined use of CRISPR-based genome engineering tools and modern genomic-assisted breeding approaches are gaining momentum in identifying genetic determinants of complex traits for crop improvement.This review provides a synopsis of plant responses to drought and salinity stresses at the morphological,physiological,and molecular levels.We also highlight recent advances in CRISPR-based tools and their use in understanding the multi-level nature of plant adaptations to drought and salinity stress.Integrating CRISPR tools with modern breeding approaches is ideal for identifying genetic factors that regulate plant stress-response pathways and for the introgression of beneficial traits to develop stress-resilient crops. | Rahul Mahadev Shelake Ulhas Sopanrao Kadam Ritesh Kumar Dibyajyoti Pramanik Anil Kumar Singh Jae-Yean Kim | 2022 | Plant Communications2022，3，6： | 0 |
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