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| 1 | Molecular immune pathogenesis and diagnosis of COVID-19显示文摘Coronavirus disease 2019(COVID-19)is a kind of viral pneumonia which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The emergence of SARS-CoV-2 has been marked as the third introduction of a highly pathogenic coronavirus into the human population after the severe acute respiratory syndrome coronavirus(SARS-CoV)and the Middle East respiratory syndrome coro-navirus(MERS-CoV)in the twenty-first century.In this minireview,we provide a brief introduction of the general features of SARS-CoV-2 and discuss current knowledge of molecular immune pathogenesis,diagnosis and treatment of COVID-19 on the base of the present understanding of SARS-CoV and MERS-CoV infections,which may be helpful in offering novel insights and potential therapeutic targets for combating the SARS-CoV-2 infection. | Xiaowei Li Manman Geng Yizhao Peng Liesu Meng Shemin Lu | 2020 | Journal of Pharmaceutical Analysis2020，10，2： | 31 |
| 2 | Preparation of monoclonal antibody against human KIAA0100 protein and Northern blot analysis of human KIAA0100 gene显示文摘Monoclonal antibodies(MAbs) are important tools for the study of proteins′ function and structure. But there has been no report on the preparation of MAbs against human KIAA0100 protein up to date. Here, first, we generated the mouse MAb against human KIAA0100 protein using purified recombinant 6×Histidinc(6×His)-tagged human KIAA0100 protein segment(1557–2234) as an antigen; then, the m RNA expression of human KIAA0100 gene was detected in U937 cells using Northern blot analysis. The results showed that the mouse MAb against human KIAA0100 protein could sensitively recognize the human KIAA0100 protein using Western blot analysis and immunocytochemistry analysis. Besides, Western blot analysis revealed that human KIAA0100 gene possibly encoded two different protein products(254 k Da and < 250 k Da) in U937 cells. Moreover,Northern blot analysis confirmed that human KIAA0100 gene might produced two different m RNA products(6000–10000 bp and 5000–6000 bp) in U937 cells. The results provide a basis for large-scale production of the MAb against human KIAA0100 protein, which will be useful for the study of human KIAA0100 protein′s function/structure and MAb-targeted drugs in the future. | He Cui Xi Lan Shemin Lu Fujun Zhang Wanggang Zhang | 2017 | Journal of Pharmaceutical Analysis2017，7，3： | 4 |
| 3 | Molecular detection of SARS-CoV-2 being challenged by virus variation and asymptomatic infection显示文摘Coronavirus disease 2019(COVID-19)has been a pandemic for more than a year.With the expanding second wave of the pandemic in winter,the continuous evolution of SARS-CoV-2 has brought new issues,including the significance of virus mutations in infection and the detection of asymptomatic infection.In this review,we first introduced several major SARS-CoV-2 mutations since the COVID-19 outbreak and then mentioned the widely used molecular detection techniques to diagnose COVID-19,primarily focusing on their strengths and limitations.We further discussed the effects of viral genetic variation and asymptomatic infection on the molecular detection of SARS-CoV-2 infection.The review finally summarized useful insights into the molecular diagnosis of COVID-19 under the special situation being challenged by virus mutation and asymptomatic infection. | Congshan Jiang Xiaowei Li Changrong Ge Yuanyuan Ding Tao Zhang Shuai Cao Liesu Meng Shemin Lu | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 2 |
| 4 | A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering显示文摘Rheumatoid arthritis(RA)is exacerbated by TNF-alpha signaling.However,it remains unclear whether TNF-α-activated TNFR1 and TNFR2 are regulated by extracellular factors.Here,we showed that soluble glycosylated interleukin-17 receptor D(sIL-17RD),which was produced by proteolytic cleavage,enhanced TNF-α-induced RA.We revealed that IL-17RD shedding was induced by the proteolytic enzyme TACE and enhanced by TNF-αexpression in macrophages.Intriguingly,sIL-17RD was elevated in the sera of arthritic mice and rats.Recombinant sIL-17RD significantly enhanced the TNF-α-induced proinflammatory response by promoting TNF-α-TNFR-sIL-17RD complex formation and receptor clustering,leading to the accelerated development of collagen-induced arthritis.Our observations revealed that ectodomain shedding of IL-17RD occurred in RA to boost the TNF-α-induced inflammatory response.Targeting sIL-17RD may provide a new strategy for the therapy of RA. | Sihan Liu Yanxia Fu Kunrong Mei Yinan Jiang Xiaojun Sun Yinyin Wang Fangli Ren Congshan Jiang Liesu Meng Shemin Lu Zhihai Qin Chen Dong Xinquan Wang Zhijie Chang Shigao Yang | 2021 | Cellular & Molecular Immunology2021，18，8： | 1 |
| 5 | Bioinformatic prediction and functional characterization of human KIAA0100 gene显示文摘Our previous study demonstrated that human KIAA0100 gene is a novel acute monocytic leukemia-associated antigen(MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown to date. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using online software;Secondly, human KIAA0100 gene expression was downregulated by the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas) 9 system in U937 cells. Cell proliferation and apoptosis were next evaluated in KIAA0100-knockdown U937 cells. The bioinformatic prediction showed that human KIAA0100 gene was located on 17q11.2, and human KIAA0100 protein was located in the secretory pathway. Besides, human KIAA0100 protein contained a signal peptide, a transmembrane region, three types of secondary structures(alpha helix, extended strand, and random coil), and four domains from mitochondrial protein 27(FMP27). The observation on functional characterization of human KIAA0100 gene revealed that its downregulation inhibited cell proliferation, and promoted cell apoptosis in U937 cells. To summarize, these results suggest human KIAA0100 gene possibly comes within mitochondrial genome; moreover, it is a novel anti-apoptotic factor related to carcinogenesis or progression in acute monocytic leukemia, and may be a potential target for immunotherapy against acute monocytic leukemia. | He Cui Xi Lan Shemin Lu Fujun Zhang Wanggang Zhang | 2017 | Journal of Pharmaceutical Analysis2017，7，1： | 1 |
| 6 | GP×1 knockdown suppresses chondrogenic differentiation of ATDC5 cells through induction of reductive stress显示文摘谷胱甘肽 peroxidase (GPx1 ) 1 是包含蛋白质的硒(Se ) 并且在软骨形成被导致。GPx1 消除反应的氧种类(ROS ) ,它为 chondrogenic 正式就职被要求。在软骨和包含的氧化还原作用机制的 GPx1 的生理的性质不被知道。ATDC5 房间的 chondrogenic 区别上的 GPx1 的效果通过短发卡被检验调停 RNA 的基因 silencing。结果表明了那 GPx1 决定区域 Y 盒子的性的击倒的损害基因表示 9,骨胶原 II (关口 II ) ,和 aggrecan。GPx1 击倒压制了软骨 glycosaminoglycans 的累积(作呕) 并且 chondrocyte 的增长。GPx1 击倒的也导致的房间 apoptosis。然而,向外长的氧化应力的房间敏感没在 GPx1 以后被增加击倒。出人意料地, GPx1 击倒不仅 ROS 的增加的生产描绘而且引起了减少的应力的导致的氧化应力由谷胱甘肽(GSH ) 的举起显示了 /oxidized GSH (GSSG ) 比率。而且,减少的 GPx1 击倒调停和氧化应力能被氧化 thiol 代理人 diamide 和包含 thiol 混合物 N-acetylcysteine (NAC ) 分别地反对。而且, NAC 稀释了 GPx1 击倒导致房间 apoptosis,当 diamide 阻止了 GPx1 击倒压制 chondrocyte 增长时。最后, diamide 然而并非 NAC 能救 GPx1 击倒调停损害 chondrogenic 区别。在摘要, GPx1 通过细胞内部的 GSH/GSSG 比率,而非抗氧化剂酶的调整主要为在 ATDC5 房间感应的 chondrogenic 是必要的除去 ROS。另外, GPx1 击倒导致损害了 chondrogenesis 可以由于 Se 参予地方性的 osteoarthropathy 的致病缺乏。这些观察在软骨退化期间为治疗学的目标的发展提供新奇卓见。 | Jidong Yan Yuanxu Guo Yao Fei RuiZhang Yan Han Shemin Lu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，2： | 0 |
| 7 | Evaluation of immunoprotection against coronavirus disease 2019:Novel variants,vaccine inoculation,and complications显示文摘The strikingly rapidly mutating nature of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)genome has been a constant challenge during the coronavirus disease 2019(COVID-19)pandemic.In this study,various techniques,including reverse transcription-quantitative polymerase chain reaction,antigen-detection rapid diagnostic tests,and high-throughput sequencing were analyzed under different scenarios and spectra for the etiological diagnosis of COVID-19 at the population scale.This study aimed to summarize the latest research progress and provide up-to-date understanding of the methodology used for the evaluation of the immunoprotection conditions against future variants of SARS-CoV-2.Our novel work reviewed the current methods for the evaluation of the immunoprotection status of a specific population(endogenous antibodies)before and after vaccine inoculation(administered with biopharmaceutical antibody products).The present knowledge of the immunoprotection status regarding the COVID-19 complications was also discussed.Knowledge on the immunoprotection status of specific populations can help guide the design of pharmaceutical antibody products,inform practice guidelines,and develop national regulations with respect to the timing of and need for extra rounds of vaccine boosters. | Congshan Jiang Kaichong Jiang Xiaowei Li Ning Zhang Wenhua Zhu Liesu Meng Yanmin Zhang Shemin Lu | 2023 | Journal of Pharmaceutical Analysis2023，13，1： | 0 |
| 8 | Design,synthesis,and evaluation of fluoroquinolone derivatives as microRNA-21 small-molecule inhibitors显示文摘MicroRNA-21(miRNA-21)is highly expressed in various tumors.Small-molecule inhibition of miRNA-21 is considered to be an attractive novel cancer therapeutic strategy.In this study,fluoroquinolone derivatives A1eA43 were synthesized and used as miRNA-21 inhibitors.Compound A36 showed the most potent inhibitory activity and specificity for miRNA-21 in a dual-luciferase reporter assay in HeLa cells.Compound A36 significantly reduced the expression of mature miRNA-21 and increased the protein expression of miRNA-21 target genes,including programmed cell death protein 4(PDCD4)and phosphatase and tensin homology deleted on chromosome ten(PTEN),at 10 μM in HeLa cells.The Cell Counting Kit-8 assay(CCK-8)was used to evaluate the antiproliferative activity of A36;the results showed that the IC_(50) value range of A36 against six tumor cell lines was between 1.76 and 13.0 μM.Meanwhile,A36 did not display cytotoxicity in BEAS-2B cells(lung epithelial cells from a healthy human donor).Furthermore,A36 significantly induced apoptosis,arrested cells at the G_(0)/G_(1) phase,and inhibited cell-colony formation in HeLa cells.In addition,mRNA deep sequencing showed that treatment with A36 could generate 171 dysregulated mRNAs in HeLa cells,while the expression of miRNA-21 target gene dual-specificity phosphatase 5(DUSP5)was significantly upregulated at both the mRNA and protein levels.Collectively,these findings demonstrated that A36 is a novel miRNA-21 inhibitor. | Yuan-Yuan Hei Si Wang Xiao-Xiao Xi Hai-Peng Wang Yuanxu Guo Minhang Xin Congshan Jiang Shemin Lu San-Qi Zhang | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 0 |
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