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| 1 | Alisol B 23-acetate-induced HepG2 hepatoma cell death through mTOR signaling-initiated G_1 cell cycle arrest and apoptosis: A quantitative proteomic study显示文摘Objective: The present study aimed to investigate the molecular events in alisol B 23-acetate(ABA) cytotoxic activity against a liver cancer cell line.Methods: First, we employed a quantitative proteomics approach based on stable isotope labeling by amino acids in cell culture(SILAC) to identify the different proteins expressed in HepG2 liver cancer cells upon exposure to ABA. Next, bioinformatics analyses through DAVID and STRING on-line tools were used to predict the pathways involved. Finally, we applied functional validation including cell cycle analysis and Western blotting for apoptosis and mTOR pathway-related proteins to confirm the bioinformatics predictions.Results: We identified 330 different proteins with the SILAC-based quantitative proteomics approach. The bioinformatics analysis and the functional validation revealed that the mTOR pathway, ribosome biogenesis, cell cycle, and apoptosis pathways were differentially regulated by ABA. G1 cell cycle arrest, apoptosis and mTOR inhibition were confirmed.Conclusions: ABA, a potential mTOR inhibitor, induces the disruption of ribosomal biogenesis. It also affects the mTOR-MRP axis to cause G1 cell cycle arrest and finally leads to cancer cell apoptosis. | Ji Xia Qiang Luo Shengbin Huang Fuquan Jiang Lin Wang Guanghui Wang Jingjing Xie Jie Liu Yang Xu | 2019 | Chinese Journal of Cancer Research2019，31，2： | 2 |
| 2 | Transcription factor EB(TFEB)-mediated autophagy protects bovine mammary epithelial cells against H_(2)O_(2)-induced oxidative damage in vitro显示文摘Background:Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy.Transcription factor EB(TFEB)-mediated autophagy is an important cytoprotective mechanism against oxidative stress.However,effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain unknown.Therefore,the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative stress.Results:H_(2)O_(2) challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II,increased number of autophagosomes and autolysosomes while decreased protein abundance of p62.Inhibition of autophagy via bafilomycin A1 aggravated H_(2)O_(2)-induced reactive oxygen species(ROS)accumulation and apoptosis in MAC-T cells.Furthermore,H_(2)O_(2) treatment triggered the translocation of TFEB into the nucleus.Knockdown of TFEB by siRNA reversed the effect of H_(2)O_(2) on protein abundance of LC3-II and p62 as well as the number of autophagosomes and autolysosomes.Overexpression of TFEB activated autophagy and attenuated H_(2)O_(2)-induced ROS accumulation.Furthermore,TFEB overexpression attenuated H_(2)O_(2)-induced apoptosis by downregulating the caspase apoptotic pathway.Conclusions:Our results indicate that activation of TFEB mediated autophagy alleviates H_(2)O_(2)-induced oxidative damage by reducing ROS accumulation and inhibiting caspase-dependent apoptosis. | Xudong Sun Renxu Chang Yan Tang Shengbin Luo Chunhui Jiang Hongdou Jia Qiushi Xu Zhihao Dong Yusheng Liang Juan J.Loor Chuang Xu | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 1 |
| 3 | Latest progresses and the application of various electrolytes in high-performance solid-state lithium-sulfur batteries显示文摘With the emergence of some solid electrolytes(SSEs)with high ionic conductivity being comparable to liquid electrolytes,solid-state lithium-sulfur batteries(SSLSBs)have been widely regarded as one of the most promising candidates for the next generation of power generation energy storage batteries,and have been extensively researched.Though many fundamental and technological issues still need to be resolved to develop commercially viable technologies,SSLSBs using SSEs are expected to address the present limitations and achieve high energy and power density while improving safety,which is very attractive to large-scale energy storage systems.SSLSBs have been developed for many years.However,there are few systematic discussions related to the working mechanism of action of various electrolytes in SSLSBs and the defects and the corresponding solutions of various electrolytes.To fill this gap,it is very meaningful to review the recent progress of SSEs in SSLSBs.In this review,we comprehensively investigate and summarize the application of SSEs in LSBs to determine the differences which still exist between current progresses and real-world requirements,and comprehensively describe the mechanism of action of SSLSBs,including lithium-ion transport,interfacial contact,and catalytic conversion mechanisms.More importantly,the selection of solid electrolyte materials and the novel design of structures are reviewed and the properties of various SSEs are elucidated.Finally,the prospects and possible future research directions of SSLSBs including designing high electronic/ionic conductivity for cathodes,optimizing electrolytes and developing novel electrolytes with excellent properties,improving electrode/-electrolyte interface stability and enhancing interfacial dynamics between electrolyte and anode,using more advanced test equipment and characterization techniques to analyze conduction mechanism of Li^(+)in SSEs are presented.It is hoped that this review can arouse people’s attention and enlighten the development of functional materials and novel structures of SSEs in the next step. | Yanan Li Nanping Deng Hao Wang Qiang Zeng Shengbin Luo Yongbing Jin Quanxiang Li Weimin Kang Bowen Cheng | 2023 | Journal of Energy Chemistry2023，，7： | 0 |
| 4 | Inhibiting nuclear factor erythroid 2 related factor 2-mediated autophagy in bovine mammary epithelial cells induces oxidative stress in response to exogenous fatty acids显示文摘Background:In early lactation,bovine mammary epithelial cells undergo serious metabolic challenges and oxidative stress both of which could be alleviated by activation of autophagy.Nuclear factor erythroid 2 related factor 2(NFE2L2),a master regulator of cellular redox homeostasis,plays an important role in the regulation of autophagy and oxidative stress.Thus,the objective of this study was to investigate the role of NFE2L2-mediated autophagy on oxidative stress of bovine mammary epithelial cells in response to exogenous free fatty acids(FFA).Results:Exogenous FFA induced linear and quadratic decreases in activities of glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD),and increases in the contents of reactive oxygen species(ROS)and malondialdehyde(MDA).Protein abundance of LC3-phosphatidylethanolamine conjugate(LC3-Ⅱ)and the number of autophagosomes and autolysosomes decreased in a dose-dependent manner,while protein abundance of p62 increased in cells challenged with FFA.Activation of autophagy via pre-treatment with Rap attenuated the FFAinduced ROS accumulation.Importantly,FFA inhibited protein abundance of NFE2L2 and the translocation of NFE2L2 into the nucleus.Knockdown of NFE2L2 by siRNA decreased protein abundance of LC3-Ⅱ,while it increased protein abundance of p62.Furthermore,sulforaphane(SFN)pre-treatment attenuated the FFA-induced oxidative stress by activating NFE2L2-mediated autophagy.Conclusions:The data suggested that NFE2L2-mediated autophagy is an important antioxidant mechanism in bovine mammary epithelial cells experiencing increased FFA loads. | Renxu Chang Xudong Sun Hongdou Jia Qiushi Xu Zhihao Dong Yan Tang Shengbin Luo Qianming Jiang Juan J.Loor Chuang Xu | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 0 |
| 5 | 2D-polyimide film sensitized monolayer MoS_(2) phototransistor enabled near-infrared photodetection显示文摘Two-dimensional(2D)transition metal dichalcogenides(TMDCs)-based heterostructures open the door to fabricate various promising hybrid photodetectors,while it is still a challenge to achieve excellent and stable near-infrared(NIR)photoresponse.Here,a MoS_(2)–2DPI(2D-polyimide(2DPI))heterojunction-based phototransistor(HPT)was fabricated.Near-infrared photodetection with excellent performance has been realized.This HPT exhibited a photoresponsivity of 390.5 A/W,a specific detectivity of 5.10×10^(12)Jones,a photogain 1.04×10^(5),and a photoresponse rise and decay time of 400 and 430 ms(λ=900 nm,P=16.2μW/cm^(2)),respectively.It also shows a broadband wavelength response from 405 to 1,020 nm.This superior performance could be attributed to the strong near-infrared absorption and the type-II(staggered)band alignment which ensures efficient charge transfer from 2DPI to MoS_(2).The face-to-face spatial configuration of MoS_(2)–2DPI heterostructures ensures efficient transfer of photoinduced carriers through the interface,electron and holes can be separated due to the large band offsets.This work presents a significant step for the manipulation of high-performance NIR photodetector of twodimensional covalent organic polymer-sensitized monolayer TMDCs. | Qingqing Luo Guangyuan Feng Yaru Song Enbing Zhang Jiangyan Yuan Dejuan Fa Qisheng Sun Shengbin Lei Wenping Hu | 2022 | Nano Research2022，15，9： | 0 |
| 6 | Construction and nanotribological study of a glassy covalent organic network on surface显示文摘Unraveling the nature of complex condensed matter systems is of paramount importance in a variety of fields such as pharmacology and materials science.Here we report the synthesis,by the dynamic covalent chemistry(DCC),of a robust,continuous,and low-defect glassy covalent organic network(GCON).The direct imaging of the molecular structure clearly shows the amorphous nature of GCONs,which consists with the competing(nano)crystallite model,not Zachariasen continuous random networks(Z-CRN).Remarkably,the microscopic friction properties were measured on GCONs by atomic force microscopy(AFM),and the GCONs showed lower friction force in comparison with crystalline covalent organic frameworks(COFs). | Guangyuan Feng Qingqing Luo Mengqi Li Yaru Song Yongtao Shen Shengbin Lei Wenping Hu | 2022 | Nano Research2022，15，5： | 0 |
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