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| 1 | B7-DC (PD-L2) costimulation of CD4^(+) T-helper 1 response via RGMb显示文摘The role of B7-DC in T-cell responses remains controversial because both coinhibitory and costimulatory functions have been reported in various experimental systems in vitro and in vivo.In addition to interacting with the coinhibitory receptor PD-1,B7-DC has also been shown to bind repulsive guidance molecule b(RGMb).The functional consequences of the B7-DC/RGMb interaction,however,remain unclear.More than a decade ago,we reported that replacement of a murine B7-DC mutant lysine with serine(K113S)at positive 113 resulted in a loss of binding capacity to PD-1.Nevertheless,K113S remained costimulatory for T cells in vitro,implicating a dual functionality for B7-DC in T-cell responses.Here we show that recombinant K113S protein interacts with RGMb with a similar affinity to wild-type B7-DC.More importantly,K113S costimulates CD4^(+)T-cell responses via RGMb and promotes Th1 polarization.RGMb is expressed on the surface of naive mouse T cells,macrophages,neutrophils and dendritic cells.Finally,K113S/RGMb costimulation suppresses Th2-mediated asthma and ameliorates small airway inflammation and lung pathology in an experimental mouse model.Our findings indicate that RGMb is a costimulatory receptor for B7-DC.These findings from the K113S variant provide not only a possible explanation for the B7-DC-triggered contradictory effects on T-cell responses,but also a novel approach to investigate the B7-DC/PD-1/RGMb axis.Recombinant K113S or its derivatives could potentially be developed as an agonist for RGMb to costimulate the Th1 response without triggering PD-1-mediated T-cell inhibition. | Xinxin Nie Wenni Chen Ying Zhu Baozhu Huang Weiwei Yu Zhanshuai Wu Sizheng Guo Yiping Zhu Liqun Luo Shengdian Wang Lieping Chen | 2018 | Cellular & Molecular Immunology2018，15，10： | 3 |
| 2 | Numerical simulation and experimental validation of gas–solid flow in the riser of a dense fluidized bed reactor显示文摘Gas–solid flow in the riser of a dense fluidized bed using Geldart B particles(sand),at high gas velocity (7.6–15.5 m/s)and with comparatively high solid flux(140–333.8 kg/m2 s),was investigated experimentally and simulated by computational fluid dynamics(CFD),both two-and three-dimensional and using the Gidaspow,O’Brien-Syamlal,Koch-Hill-Ladd and EMMS drag models.The results predicted by EMMS drag model showed the best agreement with experimental results.Calculated axial solids hold-up pro- files,in particular,are well consistent with experimental data.The flow structure in the riser was well represented by the CFD results,which also indicated the cause of cluster formation.Complex hydrody- namical behaviors of particle cluster were observed.The relative motion between gas and solid phases and axial heterogeneity in the three subzones of the riser were also investigated,and were found to be consistent with predicted flow structure.The model could well depict the difference between the two exit configurations used,viz.,semi-bend smooth exit and T-shaped abrupt exit.The numerical results indicate that the proposed EMMS method gives better agreement with the experimental results as compared with the Gidaspow,O’Brien-Syamlal,Koch-Hill-Ladd models.As a result,the proposed drag force model can be used as an efficient approach for the dense gas–solid two-phase flow. | Xueyao Wang Fan Jiang xiang xu Shengdian Wang Xuezhi Wu Baoguo Fan Liangliang Liao Jiachang Wang Yunhan Xiao | 2009 | Particuology2009，7，4： | 3 |
| 3 | IL-21 accelerates xenogeneic graft-versus-host disease correlated with increased B-cell proliferation显示文摘Graft-versus-host disease(GVHD)is a prevalent and potential complication of hematopoietic stem cell trans-plantation.An animal model,xenogeneic GVHD(X-GVHD),that mimics accurately the clinical presentation of GVHD would provide a tool for investigating the mechanism involved in disease pathogenesis.Murine models indi-cated that inhibiting IL-21 signaling was a good therapy to reduce GVHD by impairing T cell functions.We sought to investigate the effect of exogenous human IL-21 on the process of X-GVHD.In this study,human IL-21 was expressed by hydrodynamic gene delivery in BALB/c-Rag2−/−IL-2Rγc−/−(BRG)immunodeficient mice which were intravenously transplanted human peripheral blood mononuclear cells(hPBMCs).We found that human IL-21 exacerbated X-GVHD and resulted in rapid fatality.As early as 6 days after hPBMCs transplanted to BRG mice,a marked expansion of human CD19+B cells,but not T cells,was observed in spleen of IL-21-treated mice.Com-pared with control group,IL-21 induced robust immuno-globulin secretion,which was accompanied by increased accumulation of CD19+CD38high plasma cells in spleen.In addition,we demonstrated that B-cell depletion was able to ameliorate X-GVHD.These results are the fi rst to fi nd in vivo expansion and differentiation of human B cells in response to IL-21,and reveal a correlation between the expansion of B cells and the exacerbation of xenogeneic GVHD.Our fi ndings show evidence of the involvement of B cells in X-GVHD and may have implications in the treat-ment of the disease. | Xiaoran Wu Yi Tan Qiao Xing Shengdian Wang | 2013 | Protein & Cell2013，4，11： | 2 |
| 4 | Co-signaling molecules of the B7-CD28 family in positive and negative regulation of T lymphocyte response显示文摘 | Shengdian Wang Liping Chen | 2004 | Microbes Infec2004，，： | 1 |
| 5 | Solids Acceleration Length in a Cold Dense Transport Bed显示文摘The solids acceleration length was investigated in a cold dense transport bed(0.10m-ID×17m-height)with three kinds of silica sand.The solids circulation rate(Gs)was up to 954 kg/(m2s).The effects of operating conditions,particle properties,and riser structures on the solids acceleration length were investigated under high Gs conditions,with the effect of riser height non-negligible.The solids acceleration length increased with the increase of the riser height.Based on the experimental data,an empirical correlation was proposed to predict the solids acceleration length.Predictions of the correlation were in good agreement with the experimental data in this work and those from the literature over a wide range of Gs(18~954kg/(m2s)). | Yunhan Xiao | 2012 | Journal of Thermal Science2012，21，6： | 1 |
| 6 | Co - signaling molecules of the B7 - CD28 family in positive and negative reguLation of T lymphocyte response 显示文摘 | Shengdian Wang Liping Chen | 2004 | Microbes Infec2004，，： | 1 |
| 7 | Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction显示文摘 | Shengdian Wang Jürgen Bajorath Flies D B | 2003 | J Exper Med2003，197，9： | 1 |
| 8 | The tumor immunosuppressive microenvironment impairs the therapy of anti-HER2/neu antibody显示文摘It has been well established that immune surveillance plays critical roles in preventing the occurrence and prog-ression of tumor.More and more evidence in recent years showed the host anti-tumor immune responses also play important roles in the chemotherapy and radiotherapy of cancers.Our previous study found that tumor-targeting therapy of anti-HER2/neu mAb is mediated by CD8+T cell responses.However,we found here that enhancement of CD8+T cell responses by combination therapy with IL-15R/IL-15 fusion protein or anti-CD40,which are strong stimultors for T cell responses,failed to promote the tumor therapeutic effects of anti-HER2/neu mAb.Analysis of tumor microenviornment showed that tumor tissues were heavily infiltrated with the immunosuppressive macrophages and most tumor infiltrating T cells,especially CD8^(+)T cells,expressed high level of inhibitory co-signaling receptor PD-1.These data suggest that tumor microenvironment is dominated by the immunosuppressive strategies,which thwart anti-tumor immune responses.Therefore,the successful tumor therapy should be the removal of inhibitory signals in the tumor microenvir-onment in combination with other therapeutic strategies. | Meng Xu Xuexiang Du Mingyue Liu Sirui Li Xiaozhu Li Yang-Xin Fu Shengdian Wang | 2012 | Protein & Cell2012，3，6： | 1 |
| 9 | CD8^+ T cell response mediates the therapeutic effects of oncolytic adenovirus in an immunocompetent mouse model显示文摘The role of anti-tumor immune responses in oncolytic adenoviral therapy has not been well studied due to lack of efficacious tu- mor model in immunocompetent mice.Here,we evaluated the contributions of immune components to the therapeutic effects of oncolytic adenoviruse in an immunocompetent murine tumor model permissive for infection and replication of adenovirus.We found that CD8+T cells were critical mediator for antitumor efficacy by oncolytic adenovirus.Intratumoral viral therapy induced intensive infiltration of CD8+T cells in tumor,increased tumor-specific IFN-?(interferon-?)production and CTL(cytotoxic T lymphocyte)activity of lymphocytes,and generated a long-term tumor-specific immune memory.Boosting CD8+T cell responses by agonistic anti-4-1BB(cluster differentiation 137,CD137)antibody showed synergistic anticancer effects with oncolytic viro- therapy.Our results provide insight into antitumor mechanisms of oncolytic adenovirus in addition to their direct oncolytic effect. | YANG YaJun LI XiaoZhu WANG YaoHe WANG ShengDian | 2012 | Chinese Science Bulletin2012，57，1： | 1 |
| 10 | The development and functions of CD41 T cells expressing a transgenic TCR specific for an MHC-I-restricted tumor antigenic epitope显示文摘It has been reported that the ratio of CD41 to CD81 T cells has no bias in a few class I major histocompatibility complex(MHC-I)-restricted T-cell receptor(TCR)-transgenic mice specific for alloantigens or autoantigens,in which most CD41 T cells express an MHC-I-restricted TCR.In this study,we further showed that more than 50%of CD41 T cells in MHC-I-restricted P1A tumor antigen-specific TCR(P1ATCR)-transgenic mice could specifically bind to MHC-I/P1A peptide complex.P1A peptide could stimulate the transgenic CD41 T cells to proliferate and secrete both type 1 helper T cell and type 2 helper T cell cytokines.The activated CD41 T cells also showed cytotoxicity against P1A-expressing tumor cells.The analysis of TCR a-chains showed that these CD41 T cells were selected by co-expressing endogenous TCRs.Our results show that CD41 T cells from P1ATCR transgenic mice co-expressed an MHC-I-restricted transgenic TCR and another rearranged endogenous TCRs,both of which were functional. | Xue Han Peiying Ye Liqun Luo Linghua Zheng Yang Liu Lieping Chen Shengdian Wang | 2011 | Cellular & Molecular Immunology2011，8，4： | 0 |
| 11 | Anti-TOSO antibody treatment promotes T cell activation-induced cell death(AICD) in vitro and in vivo显示文摘T cell activation-induced cell death(AICD),that involves the induction of Fas-mediated apoptosis,is very important for the maintenance of immune homeostasis.TOSO was firstly described as an inhibitor of Fasmediated apoptosis and overexpressed in chronic lymphocytic leukemia.Recently,TOSO was identified as IgM FcR.In this study,we produced anti-TOSO monoclonal antibody(mAb)that could block the binding of IgM to TOSO and found that T cell apoptosis is negatively correlated with TOSO expression during T cell activation.Treatment of activated T cells with anti-TOSO blocking mAb promoted T cell AICD in in vitro AICD model,and treatment of xenogeneic-GVHD mice with the antibody also increased the sensitivity of activated T cells to Fasinduced apoptosis,which was accompanied by reduction of c-FLIPL expression and up-regulation of AP-1 complex.In summary,our data indicate the anti-apoptotic effect of TOSO in T cell AICD and open up new therapeutic prospects for the treatment of hematologic malignancies and immune disorders. | Yi Tan Xue Han Xiaoran Wu Qiao Xing Lieping Chen Shengdian Wang | 2014 | Chinese Science Bulletin2014，59，13： | 0 |
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