| 1 | Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocataiysts显示文摘We have developed a whole-cell bioconversion system for the production ofD-1,2,4-butanetriol (BT)from renewable biomass.A plasmid pETduet-xylB-yjhG-T7- adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3)to obtain the whole cells of E.coli BL2I-XYMA capable of bioconversion D-xylose to BT.Then,the factors including carbon sources,nitrogen sources,metal ions,and culture conditions (pH,temperature,IPTG)were identified,and their effects on the wholecell activity for BT production were investigated.To obtain the highest whole-cell activity,the optimal cultivation parameters are:15g·L^-1 yeast extract,5g·L^-1 sucrose, 3g·L^-1 KH2PO4,5g·L^-1 NaCl,3g·L^-1 NH4Cl,0.25g·L^-1 MgSO4·7H2O and 1mL·L^-1 the mixture of trace elements.With the optimized whole cells of E.coli BL21-XYMA,60g·L^-1 of xylose was converted to 28g·L^-1 BT with a molar yield of 66%,which is higher than those reported in the biotechnological system. | Shewei Hu Qian Gao Xin Wang Jianming Yang Nana Xu Kequan Chen Sheng Xu Pingkai Ouyang | 2018 | Frontiers of Chemical Science and Engineering2018，12，4： | 1 |
| 2 | Formation of Face‑Centered Cubic Phase in Ti35 Alloy Under In Situ Heating Transmission Electron Microscopy显示文摘A thermally induced hexagonal close-packed(HCP)to face-centered cubic(FCC)phase transition was investigated in anα-type Ti35 alloy with twinned structure by in situ heating transmission electron microscopy(TEM)and ab initio calculations.TEM observations indicated that the HCP to FCC phase transition occurred both within matrix/twin and at the twin boundaries in the thinner region of the TEM film,and the FCC-Ti precipitated as plates within the matrix/twin,while as equiaxed cells at twin boundaries.The crystallographic orientation relationship between HCP-Ti and FCC-Ti can be described as:{111}FCC//{0002}HCP and<110>FCC//<1210>HCP.The HCP to FCC phase transition was accomplished by forming an intermediate state with a BB stacking sequence through the slip of partial dislocations.The formation of such FCC-Ti may be related to the thermal stress and temperature.Ab initio calculations showed that the formation of FCC-Ti may also be related to the contamination of interstitial atoms such as oxygen. | Jianan Hu Mengmeng Yang Wenlong Xiao Hao Wang Dehai Ping Chengze Liu Shewei Xin Songquan Wu Kai Zhang Yi Yang Lai‑Chang Zhang Aijun Huang | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，3： | 0 |
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