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| 1 | A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)显示文摘Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of great agricultural, nutritional, and economic importance. However, the genetic basis of CEs on SOC (CE-SOC) remains enigmatic. In this study, we use an optimized approach to sequence the cytoplasmic (plastid and mitochondrial) genomes of allotetraploid oilseed rape (Brassica napus) cultivars, 51218 and 56366, that bear contrasting CE-SOC. By combining comparative genomics and genome-wide transcriptome analysis, we identify mitochondria-encoded orf188 as a potential CE-SOC determinant gene. Functional analyses in the model system Arabidopsis thaliana and rapeseed demonstrated that orf188 governs CE-SOC and could significantly increase SOC, strikingly, through promoting the yield of ATP. Consistent with this finding, transcriptional profiling with microarray and RNA sequencing revealed that orf188 affects transcriptional reprogramming of mitochondrial energy metabolism to facilitate ATP production. Intriguingly, orf188 is a previously uncharacterized chimeric gene, and the presence of this genetic novelty endows rapeseed with positive CE-SOC. Our results shed light on the molecular basis of CEs on a key quantitative trait in polyploid crops and enrich the theory of maternal control of oil content, providing new scientific guidance for breeding high-oil rapeseed germplasms. | Jun Liu Wanjun Hao Jing Liu Shihang Fan Wei Zhao Linbing Deng Xinfa Wang Zhiyong Hu Wei Hua Hanzhong Wang | 2019 | Molecular Plant2019，12，4： | 5 |
| 2 | Effective method for isolating liquid endosperm and improved procedure for subsequent DNA extraction in Brassica napus显示文摘 | Jun LI Shihang FAN Xingchao SUN Zhiyong HU Jing LIU Xiaoyi ZHU Ming ZHENG Fengming SUN Hongli YANG Liang ZHANG Linbin DENG Hongfang LIU Wei HUA | 2016 | Oil Crop Science2016，1，2： | 0 |
| 3 | Synthesis and characteristic of a novel green fluorescent protein eYGFPuv显示文摘Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluorescence was achieved in transgenic petunia, 3 expression cassettes (about 8 kb) complicate its application. In this study, to confirm whether 1 expression cassette could be used as a transgenic marker in prokaryotes and eukaryotes, eYGFPuv was cloned into prokaryotic expression vector pET28α-eYGFPuv- His and plant binary expression vector 35S::eYGFPuv. Compared to EGFP, eYGFPuv protein exhibited stronger dazzling green fluorescence in E. coli under excited light at 365 nm and maintains steadily over a long period of time without degradation. When transiently expressed in tobacco leaves, eYGFPuv protein displayed strong green fluorescence. Moreover, the fluorescence of eYGFPuv protein also could be directly observed in living plant, and thus can be used easily as a marker to screen transformed lines in transgenic research. Overall, compared to previous studies on eYGFPuv tandem repeats, our data confirmed that single eYGFPuv sequence still possesses high fluorescence intensity and quenching resistance. Furthermore, because of small size of expression cassette,it is suitable for efficient transformation in both prokaryotic and eukaryotic organisms. | Shihang Fan Linbin Deng Hongli Yang Liang Zhang Xingchao Sun Jinglin Liu Jing Liu | 2019 | Oil Crop Science2019，4，2： | 0 |
| 4 | Tetra-color superresolution microscopy based on excitation spectral demixing显示文摘Multicolor imaging allows protein colocalizations and organelle interactions to be studied in biological research,which is especially important for single-molecule localization microscopy(SMLM).Here,we propose a multicolor method called excitation-resolved stochastic optical reconstruction microscopy(ExR-STORM).The method,which is based on the excitation spectrum of fluorescent dyes,successfully separated four spectrally very close far-red organic fluorophores utilizing three excitation lasers with cross-talk of less than 3%.Dyes that are only 5 nm apart in the emission spectrum were resolved,resulting in negligible chromatic aberrations.This method was extended to three-dimensional(3D)imaging by combining the astigmatic method,providing a powerful tool for resolving 3D morphologies at the nanoscale. | Wanyan Wu Shihang Luo Chunyan Fan Tianjie Yang Shuwen Zhang Wenxiang Meng Tao Xu Wei Ji Lusheng Gu | 2023 | Light(Science & Applications)2023，12，1： | 0 |
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