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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | A new integrated genetic linkage map of the soybean显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretial and Applied Genetics2004，109，： | 1 |
| 3 | Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland 显示文摘 | MAGA E A SHOEMAKER C F ROWE J D | 2006 | Journal of Dairy Science2006，89，2： | 1 |
| 4 | Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats显示文摘 | Sepulveda J Shoemaker C B | 2008 | J Immunol Methods2008，332，12： | 1 |
| 5 | A new integrated ge-netic linkage map of the soybean显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretical and Applied Ge-netics2004，109，： | 1 |
| 6 | Lunar surface topography显示文摘 | Rennilson J J Dragg J L Morris E C Shoemaker E M Turkevich A | 1966 | Surveyor I mission report part Ⅱ:Scientific data and results NASA JPL Technical Report1966，32，： | 1 |
| 7 | Efficacy of Strepto~occus agalactiae ( group B ) vaccine in tilapia ( Oreochromis nilotlcus) by intraperitoneal and bath immersion administration 显示文摘 | Evans J J Klesius P H Shoemaker C A | 2004 | Vaccine2004，22，2728： | 1 |
| 8 | Integration of simple sequence repeat DNA markers into a soybean linkage map 显示文摘 | Akkaya M S Shoemaker R C Specht J E | 1995 | Crop Sci1995，35，5： | 1 |
| 9 | ABT-263:A Potent and orally bioavailable Bcl-2 family inhibitor显示文摘 | Tse C Shoemaker AR Adickes J | 2008 | Cancer Research2008，68，9： | 1 |
| 10 | Phylogenetic Utility of Histone H3 Intron Sequences in the Perennial Relatives of Soybean(Glycine:Leguminosae)显示文摘 | Doyle J J Kanazin V Shoemaker R C | 1996 | Mol Phylogenet Evol1996，6，： | 1 |
| 11 | A new integrated genetic linkage map of the soybean 显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretical and App lied Genetics2004，109，1： | 1 |
| 12 | Methodology for analyzing ranges of uncertain model parameters and their impact on total maximum daily load process 显示文摘 | Benaman J Shoemaker C A | 2004 | Journal of Environmental Engineering2004，130，6： | 1 |
| 13 | ABT-263: a potent and orally bioavailable Bcl-2 family inhibitor 显示文摘 | TSE C SHOEMAKER AR ADICKES J | 2008 | Cancer Res2008，68，9： | 1 |
| 14 | Molecular marker analysis of seed size in soybean显示文摘 | Hoeck J A W R Fehr R C Shoemaker | 2003 | Crop Science2003，43，： | 1 |
| 15 | Rapid detection of columnaris disease in channel catfish ( lctalurua punctauus ) with a new species - specific 16S rRNA gene - based PCR primer for flavobacterium colunmare显示文摘 | Bader J A Shoemaker C A Klesius P H | 2003 | J Microbiol Meth2003，52，： | 1 |
| 16 | Preva-lence of Streptococcus iniae in tilapia,hybrid striped bass,and channel catfish on commercial fish farms in the United States显示文摘 | SHOEMAKER C A KLESIUS P H EVANS J J | 2001 | AJVR2001，62，： | 1 |
| 17 | A new integrated ge- netic linkage map of the soybean显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretical and Applied Ge-netics2004，109，： | 1 |
| 18 | Unreliability of blood pressure and heart rate to evaluate cardiac output in emergency resuscitation and critical illness显示文摘 | Wo C J Shoemaker WC Appel PL | 1993 | Crit Care Med1993，21，2： | 1 |
| 19 | ABT-263: A potent and orally bioavailable Bcl-2 family inhibitor 显示文摘 | Tse C Shoemaker AR Adickes J | 2008 | Cancer Res2008，68，9： | 1 |
| 20 | Prevalence of Streptococcus iniae in tilapia, hybrid striped bass, and channel catfish on commercial fish farms in the United States显示文摘 | Shoemaker C A Klesius P H Evans J J | 2001 | AJVR2001，62，： | 1 |
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