|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Network pharmacology analysis to explore mechanism of Three Flower Tea against nonalcoholic fatty liver disease with experimental support using high-fat diet-induced rats显示文摘Objective:Nonalcoholic fatty liver disease(NAFLD)has become a common chronic liver disease that is harmful to human health.Moreover,there is currently no FDA-approved first-line drug for the treatment of nonalcoholic steatohepatitis(NASH)or NAFLD.Traditional Chinese medicine(TCM)is widely used to ameliorate liver diseases,such as the traditional ancient recipe called Three Flower Tea(TFT),which consists of double rose(Rosa rugosa),white chrysanthemum(Chrysanthemum morifolium),and Daidaihua(Citrus aurantium).However,the mechanisms of the action of TFT are not clear.Therefore,this study aimed to elucidate the mechanisms of TFT against NAFLD in high-fat diet(HFD)-induced rats.Methods:This study utilized bioinformatics and network pharmacology to establish the active and potential ingredient-target networks of TFT.Furthermore,a protein–protein interaction(PPI)network was constructed,and enrichment analysis was performed to determine the key targets of TFT against NAFLD.Furthermore,an animal experiment was conducted to evaluate the therapeutic effect and confirm the key targets of TFT against NAFLD.Results:A total of 576 NAFLD-related genes were searched in Gene Cards,and under the screening criteria of oral bioavailability(OB)≥30%and drug-likeness(DL)≥0.18,a total of 19 active ingredients and 210 targets were identified in TFT.Network pharmacology analysis suggested that 55 matching targets in PPIs were closely associated with roles for NAFLD treatment.Through the evaluation of network topology parameters,four key central genes,PPARγ,SREBP,AKT,and RELA,were identified.Furthermore,animal experiments indicated that TFT could reduce plasma lipid profiles,hepatic lipid profiles and hepatic fat accumulation,improve liver function,suppress inflammatory factors,and reduce oxidative stress.Through immunoblotting and immunofluorescence analysis,PPARγ,SREBP,AKT,and RELA were confirmed as targets of TFT in HFD-induced rats.Conclusion:In summary,our results indicate that TFT can prevent and treat NAFLD via multiple targets,including lipid accumulation,antioxidation,insulin sensitivity,and inflammation. | Peixuan Wu Shufei Liang Yanping He Rui Lv Bendong Yang Meng Wang Chao Wang Yong Li Xinhua Song Wenlong Sun | 2022 | Chinese Herbal Medicines2022，14，2： | 2 |
| 2 | Inhibition mechanisms of ammonia and sulfate in high-solids anaerobic digesters for food waste treatment: Microbial community and element distributions responses显示文摘The horizontal flow anaerobic digester indicated that high ammonia (2923 mg/L) and SO42-(3653 mg/L)would influence the performance of methane production with food waste as substrates.Therefore,bottle anaerobic digestion reactors were carried out to investigate the effect of ammonia/sulfate concentrations on the methane production.Experimental results manifested that the anaerobic digesters with an ammonia concentration of 3500 mg/L or sulfate of 1600 mg/L showed the best performance of methane production,with an average methane yield of 0.32 and 0.33 L (g VS)^(-1)d^(-1),respectively.Specifically,a higher ammonia (6500 mg/L) or sulfate (1600-3500 mg/L) level hindered the bioconversion of C from liquid to gas phase (2.68%or 1.73%CH_(4)-Gas,respectively),while insignificantly for the hydrolyzation of C and N from solid to liquid phase.Similar to sulfate,high ammonia nitrogen seriously inhibited the methanation process,leading to a significant carbon accumulation in the anaerobic reactor,especially for propionic acid.The predominant archaea Methanosarcina at genus level indicated that aceticlastic methanogenesis was the major methanogenic pathway.Meanwhile,high ammonia level suppressed the activity of Methanosarcina,while modest sulfate improved H_(2)-consuming methanogens activity.A large fraction of unclassified bacteria within the Firmicutes (43.78%-63.17%) and Bacteroidetes (24.20%-33.30%) phylum played an important role in substrates hydrolysis. | Likui Feng Weixin Zhao Yu Liu Yan Chen Shufei He Jing Ding Qingliang Zhao Liangliang Wei | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
| 3 | Extracellular polymeric substances-antibiotics interaction in activated sludge:A review显示文摘Antibiotics,the most frequently prescribed drugs,have been widely applied to prevent or cure human and veterinary diseases and have undoubtedly led to massive releases into sewer networks and wastewater treatment systems,a hotspot where the occurrence and transformation of antibiotic resistance take place.Extracellular polymeric substances(EPS),biopolymers secreted via microbial activity,play an important role in cell adhesion,nutrient retention,and toxicity resistance.However,the potential roles of sludge EPS related to the resistance and removal of antibiotics are still unclear.This work summarizes the composition and physicochemical characteristics of state-of-the-art microbial EPS,highlights the critical role of EPS in antibiotics removal,evaluates their defense performances under different antibiotics exposures,and analyzes the typical factors that could affect the sorption and biotransformation behavior of antibiotics.Next,interactions between microbial EPS and antibiotic resistance genes are analyzed.Future perspectives,especially the engineering application of microbial EPS for antibiotics toxicity detection and defense,are also emphatically stressed. | Weixin Zhao Jia You Shilei Yin Haizhou Yang Shufei He Likui Feng Jianju Li Qingliang Zhao Liangliang Wei | 2023 | Environmental Science and Ecotechnology2023，，1： | 0 |
| 4 | Safety evaluation of microbial pesticide (HaNPV) based on PCR method显示文摘Microbial pesticides can prevent and control diseases and pests of crops, and has become one of the important measures to ensure food and environmental safety. However, the potential harm of microbial pesticides to humans and animals is a serious concern at home and abroad. In this paper, we have investigated the infectivity and pathogenicity of a representative of viral microbial pesticides, helicoverpa armigera nuclear polyhedrosis virus (HaNPV), by specific and highly sensitive polymerase chain reaction technology. The results show that HaNPV can be gradually cleared in a short time after getting into blood of experimental rats, and does not infect other tissues or organs of animals;also indicate that the test subjects are not infectious to experimental rats after intravenous injection of HaNPV. Our method has good specificity and repeatability, and could provide an important reference for establishment of infectivity and pathogenicity detection methods for viral microbial pesticides in future. | Miao Zhao Shufei Li Qinghong Zhou Dianming Zhou Ning He Zhiyong Qian | 2019 | Frontiers of Chemical Science and Engineering2019，13，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员