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| 1 | Effect of vitamin E and human placenta cysteine peptidase inhibitor on expression of cathepsins B and L in implanted hepatoma Morris 5123 tumor model in Wistar rats显示文摘AIM: To examine the effectiveness of human placental inhibitors, by injecting vitamin E to rats with transplanted Norris-5123 hepatoma, on the expression of cathepsins B and L in tumor, liver, lung and blood sera after transplantation of Norris 5123 hepatoma.METHODS: Animals were divided into 10 groups receiving three different concentrations of vitamin E and inhibitors along or in combination and compared with negative control (healthy rats) and positive control (tumor rats). Effectiveness of treatment was evaluated with regard to survival time,tumor response and determination of the activities of proteolytic enzymes and their inhibitors using flurogenic substrates.RESULTS: Cathepsins B and L activities were elevated by 16-fold in comparison with negative control tissues, and their endogenous inhibitor activity decreased by 1.2-fold before treatment. In several cases, tumors completely disappeared following vitamin E plus human placental cyteine protease inhibitor (CPI) compared with controls.The number of complete tumor responses was higher when 20 m/kg vitamin E plus 400 μg of CPI was used, i.e.7/10 rats survived more than two mo. Cathepsins B and L were expressed significantly in tumor, liver, lung tissues and sera in parallel to the increasing of the endogenous inhibitor activity compared with the controls after treatment (P<0.0001).CONCLUSION: The data indicate formation of metastasis significantly reduced in treated rats, which might provide a therapeutic basis for anti-cancer therapy. | Tadeusz Sebzda Piotr Hanczyc Yousif Saleh Bernice F Akinpelumi Maciej Siewinski Jerzy Rudnicki | 2005 | World Journal of Gastroenterology2005，11，4： | 2 |
| 2 | Pretreatment of plantar warts with azone enhances the effect of 5- aminolevulinic acid photodynamic therapy显示文摘 | Ziolkowski P Osiecka BJ Siewinski M | 2006 | J Environ Pathol Toxicol Oncol2006，25，12： | 1 |
| 3 | Biodegradation of hard coal and its organic extract byselected microorganisms 显示文摘 | Opsipowicz B Jablonski L Siewinski A | 1994 | Fuel1994，73，12： | 1 |
| 4 | Cysteine peptidase and its inhibitor activity levels and vitamin E concentration in normal human serum and colorectal carcinomas显示文摘AIM: Cysteine peptidase (CP) and its inhibitor (CPI) are a matrix protease that may be associated with colorectal carcinoma invasion and progression, and vitamin E is also a stimulator of the immunological system. Our purpose was to determine the correlation between the expression of cysteine peptidases and their endogenous inhibitors,and the level of vitamin E in sera of patients with colorectal cancer in comparison with healthy individuals.METHODS: The levels of cysteine peptidases and their inhibitors were determined in the sera of patients with primary and metastatic colorectal carcinoma and healthy individuals using fluorogenic substrate, and the level of vitamin E was determined by HPLC.RESULTS: The levels of cysteine peptidases and their inhibitors were significantly higher in the metastatic colorectal cancer patients than that in the healthy controls (P<0.05).The activity of CP increased 2.2-fold, CPI 2.8-fold and vitamin E decreased 3.4-fold in sera of patients with metastasis in comparison with controls. The level of vitamin E in healthy individuals was higher, whereas the activity of cysteine peptidases and their inhibitors associated with complexes was lower than that in patients with cancer of the digestive tract.CONCLUSION: These results suggest that the serum levels of CP and their inhibitors could be an indicator of the prognosis for patients with metastatic colorectal cancer. Vitamin E can be administered prophylactically to prevent digestive tract neoplasmas. | Robert Szwed Zygmunt Grzebieniak Yousif Saleh Godwin Bwire Ekonjo Maciej Siewinski | 2005 | World Journal of Gastroenterology2005，11，6： | 1 |
| 5 | Pretreatment of plantar warts with azone enhances the effect of 5-aminolevulinic acid photodynamic therapy 显示文摘 | Ziolkowski P Osiecka B J Siewinski M | 2006 | J Environ Pathol Toxicol Oncol2006，25，12： | 1 |
| 6 | Uncommon type of hydroxylation of 3-alkenyl substituted derivatives of citronelloland citronellic acid by Spirodela punctata 显示文摘 | Pawlowicz P Wawrzenczyk C Siewinski A | 1992 | Phytochemistry1992，31，7： | 1 |
| 7 | Biodegradation of hard coal and its organic extract by selected microorganisms 显示文摘 | Opsipowicz B Jablonski L Siewinski A | 1994 | Fuel1994，73，： | 1 |
| 8 | Role of cysteine endopeptidases in cancerogenesis显示文摘 | Siewinski M Gutowicz J Zarzycki A Mikulewicz W | | 0，，： | 1 |
| 9 | Screening tests on the biodegradation of organic coal extract by selected fungi显示文摘 | B 0sipowicz L Jablonski A Siewinski | 1996 | Bioresource Technology1996，55，： | 1 |
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