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| 1 | An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip显示文摘CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid,portable and accurate features.However,cleavage of the amplicons and primers by the cis-and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reaction.Through phosphorothioate modification of primers,we realized onepot detection with high sensitivity using plasmids of SARS-CoV-2,HPV16 and HPV18.We also identified the activated Cas12a has a much higher affinity to C nucleotide-rich reporter than others.By applying such reporters,the reaction time required for a lateral-flow readout was significantly reduced.Furthermore,to improve the specificity of the strip-based assay,we created a novel reporter and,when combined with a customized gold-nanopaticle strip,the readout was greatly enhanced owing to the elimination of the nonspecific signal.This established system,termed Targeting DNA by Cas12a-based Eye Sight Testing in an Onepot Reaction(TESTOR),was validated using clinical cervical scrape samples for human papillomaviruses(HPVs)detection.Our system represents a general approach to integrating the nucleic acid amplification and detection into a single reaction in CRISPR-Cas systems,highlighting its potential as a rapid,portable and accurate detection platform of nucleic acids. | Jiaojiao Gong Lijuan Kan Xiuming Zhang Ying He Jiaqiang Pan Liping Zhao Qianyun Li Menghao Liu Jie Tian Sili Lin Zhouyu Lu Liang Xue Chaojun Wang Guanghui Tang | 2021 | Bioactive Materials2021，6，12： | 1 |
| 2 | Ultrasound-induced release of GDNF from lipid coated microbubbles injected into striatum reduces hypoxic–ischemic injury in neonatal rats显示文摘 | Xiaoying Wang Shenglan Guo Shuangquan Lu Jianmin Zhou Jingcai Li Sili Xia | 2012 | Brain Research Bulletin2012，，5： | 1 |
| 3 | Camitine deficiency in diabetes mellitus complications显示文摘 | Tamamo?ullari N Sili? Y I?a?asio?lu S | 1999 | J Diabetes Complications1999，13，56： | 1 |
| 4 | A model reduction approach for OFDM channel estimation under high mobility conditions 显示文摘 | Tareq Y AI-Naffouri K M Zahidul Islam Naofal AI-Dhahir Sili Lu | 2010 | IEEE Transactions on Signal Processing2010，58，4： | 1 |
| 5 | The strain-energy criterion of Iso- σθεθ line for mixed mode crack in concrete 显示文摘 | CHEN SiLi QU Kun SHI JianJun LU LiHua | 2011 | Advanced Materials Research2011，25，： | 1 |
| 6 | Promoting effect of a calcium-responsive self-assemblyβ-sheet peptide on collagen intrafibrillar mineralization显示文摘Recently,a de novo synthetic calcium-responsive self-assemblyβ-sheet peptide ID8(Ile-Asp-Ile-Asp-Ile-Asp-Ile-Asp)has been developed to serve as the template inducing hydroxyapatite nucleation.The aim of this study was to evaluate the effect of ID8 on intrafibrillar mineralization of collagen making full use of its self-assembly ability.The mineralization experiments were carried out in vitro on both bare TypeⅠcollagen and fully demineralized dentin samples.The calcium-responsive self-assembly of ID8 was revealed by circular dichroism spectrum,8-anilino-1-naphthalenesulfonic acid ammonium salt hydrate assay,attenuated total reflection Fourier transform infrared spectrum(ATR-FTIR)and transmission electron microscope(TEM).Polyacrylic acid(450 kDa)with a concentration of 100μg ml^(-1)was selected as the nucleation inhibitor based on the determination of turbidimetry and TEM with selected area electron diffraction(TEM-SAED).The results showed that collagen intrafibrillar mineralization was significantly promoted with the pretreatment of self-assembly ID8 detected by TEM-SAED,SEM,X-ray diffraction and ATRFTIR.The pretreatment of collagen utilizing self-assembly ID8 not only enhanced intermolecular hydrogen bonding but also contributed to calcium retention inside collagen and significantly increased the hydrophilicity of collagen.These results indicated that peptides with self-assembly properties like ID8 are expected to be potential tools for biomimetic mineralization of collagen. | Zhongcheng Li Qian Ren Sili Han Longjiang Ding Xi Qin Die Hu Ting He Tian Tian Ziqian Lu Linglin Zhang | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
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