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| 1 | Effect of seed sludge on characteristics and microbial community of aerobic granular sludge显示文摘Aerobic granular sludge was cultivated by using different kinds of seed sludge in sequencing batch airlift reactor.The influence of seed sludge on physical and chemical properties of granular sludge was studied;the microbial community structure was probed by using scanning electron microscope and polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).The results showed that seed sludge played an important role on the formation of aerobic granules.Seed sludge taken from beer wastewater treatment plant(inoculum A) was more suitable for cultivating aerobic granules than that of sludge from municipal wastewater treatment plant(inoculum B).Cultivated with inoculum A, large amount of mature granules formed after 35 days operation, its SVI reached 32.75 mL/g, and SOUR of granular sludge was beyond 1.10 mg/(g·min).By contrast, it needed 56 days obtaining mature granules using inoculum B.DGGE profiles indicated that the dominant microbial species in mature granules were 18 and 11 OTU when inoculum A and B were respectively employed as seed sludge.The sequencing results suggested that dominant species in mature granules cultivated by inoculum A were Paracoccus sp., Devosia hwasunensi, Pseudoxanthomonas sp., while the dominant species were Lactococcus raffinolactis and Pseudomonas sp.in granules developed from inoculum B. | Zhiwei Song Yuejun Pan Kun Zhang Nanqi Ren Aijie Wang | 2010 | Journal of Environmental Sciences2010，22，9： | 18 |
| 2 | Genetic and Molecular Characterization of H9N2 Avian Influenza Viruses Isolated from Live Poultry Markets in Hubei Province, Central China, 2013–2017显示文摘H9N2 subtype avian influenza virus(AIV)is an influenza A virus that is widely spread throughout Asia,where it jeopardizes the poultry industry and provides genetic material for emerging human pathogens.To better understand the epidemicity and genetics of H9 subtype AIVs,we conducted active surveillance in live poultry markets(LPMs)in Hubei Province from 2013 to 2017.A total of 4798 samples were collected from apparent healthy poultry and environment.Realtime RT-PCR revealed that the positivity rate of influenza A was 26.6%(1275/4798),of which the H9 subtype accounted for 50.3%(641/1275)of the positive samples.Of the 132 H9N2 viral strains isolated,48 representative strains were subjected to evolutionary analysis and genotyping.Phylogenetic analysis revealed that all H9N2 viral genes had 91.1%–100%nucleotide homology,clustered with genotype 57,and had high homology with human H9N2 viruses isolated from2013 to 2017 in China.Using a nucleotide divergence cutoff of 95%,we identified ten distinct H9N2 genotypes that continued to change over time.Molecular analysis demonstrated that six H9N2 isolates had additional potential glycosylation sites at position 218 in the hemagglutinin protein,and all isolates had I155 T and Q226 L mutations.Moreover,44 strains had A558 V mutations in the PB2 protein and four had E627 V mutations,along with H9N2 human infection strains A/Beijing/1/2016 and A/Beijing/1/2017.These results emphasize that the H9N2 influenza virus in Hubei continues to mutate and undergo mammalian adaptation changes,indicating the necessity of strengthening the surveillance of the AIV H9N2 subtype in LPMs. | Zhibin Hu Fuhu Peng Zhenghui Xiong Wanpo Zhang Tingting Li Yuejun Shi Jun Xie Xin Jin Jingjing Huang Hongde Xiao Dingren Bi Nianhua Song Zili Li | 2021 | Virologica Sinica2021，36，2： | 4 |
| 3 | Performance of Amperometric and Potentiometric Hydrogen Sensors显示文摘The principle, design, construction and performance of the amperometric and potentiometric sensors for measuring the permeation rate of hydrogen through the wall of metal equipment were investigated in order to develop a new type of hydrogen sensor with high accuracy. The transient curves of hydrogen permeation under a given charging condition were employed to evaluate the performance of two types of hydrogen sensors. The relative deviation of the hydrogen concentration detected with two types of sensors under the same condition varied from 3.0% to 13%. The accuracy, response time, reproducibility, and installation were discussed and compared. Response time of the potentiometric sensor(E-sensor) was shorter than that of the amperometric sensor(I-sensor). Both types of sensors exhibited good reproducibility. Development of I-sensor composed of a kind of proton conductor adhesives or non-fluid electrolytes which contain two functions of high electrical conductivity and a strong adhesion will be a promising prospect in order to measure hydrogen permeation at high temperature. | Yuejun Ouyang Xiaojian Wang Gang Yu Zhengwei Song Xueyuan Zhang | 2014 | Journal of Materials Science & Technology2014，30，11： | 1 |
| 4 | A Brief Introduction of Extrusion and Micro-storage Processing Technology of Corn Stover显示文摘A new corn stover roughage processing method,corn stover extrusion and micro-storage processing technology,was introduced from the aspects of basic working principle,production and processing process,characteristics of cornstalk via extrusion and micro-storage processing.The technology was proved to be a feasible and practical processing technology of stover feed with broad prospect. | Qi Hongwei Yu Wei Wu Jian Zhang Guoliang Song Yuejun | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 5 | The Numerical Simulation Research of The Remaining Oil Distribution Regulation in Test Site of Low Permeability Oil Field显示文摘1 Grid Partitioning of Test Site Static data of the well network of Gaotaizi reservoir in the test site is collected and analysed.The data of 126 wells are analyzed in detailed screening.Then make facies control interpolation calculation by the modeling software,and establish the fine geological model for numerical simulation.The grid step size is 30 meters,and the | ZHAO Yuejun SONG Kaoping FAN Guangjuan | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 0 |
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