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| 1 | Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells显示文摘OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer. | Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan | 2013 | Journal of Integrative Medicine2013，11，4： | 4 |
| 2 | Rare earth(Gd,La) co-doped ZnO nanoflowers for direct sunlight driven photocatalytic activity显示文摘In this work Gd/La@ZnO nanoflower photocatalyst was successfully synthesized by a co-precipitation method and applied for rhodamine B(Rh B) and tetracycline(TCN) degradation under direct sunlight irradiation.The doping of rare earth elements extends the optical absorption wavelength of ZnO from UV region(390 nm) to visible-light region(401 nm).In addition,the co-doped ZnO nanoflower exhibits a lower charge recombination efficiency which was confirmed by photoluminescence emission analysis.Moreover,the co-doped ZnO nanoflower exhibits the maximum degradation efficiency of 91% for Rh B and 74% for TCN under sunlight irradiation.The calculated synergistic index of co-doped ZnO is higher than that of the pure ZnO.Reactive radicals’ production was confirmed by terephthalic acid(TA) and nitro-blue tetrazolium(NBT) tests.The holes and hydroxyl(·OH) radicals play the major role in degradation reaction and it was confirmed by scavenger’s test.Moreover,the recycling test confirms the stability of the photocatalyst. | Baskaran Palanivel Romulo R.Macadangdang Jr Md Shahadat Hossain Fahad A.Alharthi Mohanraj Kumar Jih-Hsing Chang Sreedevi Gedi | 2023 | Journal of Rare Earths2023，41，1： | 3 |
| 3 | Mechanics of flow and mixing at antroduodenal junction显示文摘The morphology of tissue structures composing the pyloric orifice is thought to play a role in effectively mixing aqueous gastric effluent with duodenal secretions. To understand the physical mechanisms leading to efficient digestion requires computational models that allow for analyses of the contributions of individual structural components. Thus, we have simulated 2-D channel flows through representative models of the duodenum with moving boundary capabilities in order to quantitatively assess the importance of notable features. A well-tested flow solver was used to computationally isolate and compare geometric and kinematic parameters that lead to various characteristics of fluid motion at the antroduodenal junction. Scalar variance measurement was incorporated to quantify the mixing effectiveness of each component. It was found that the asymmetric geometry of the pyloric orifice in concert with intermittent gastric outflow and luminal constriction is likely to enhance homogenization of gastric effluent with duodenal secretions. | Seth Dillard Sreedevi Krishnan HS Udaykumar | 2007 | World Journal of Gastroenterology2007，13，9： | 2 |
| 4 | Effect of temperature on development, survival and reproduction of the mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) on cotton显示文摘 | Prasad Y G Prabhakar M Sreedevi G | 2012 | Crop Protec- tion2012，39，： | 1 |
| 5 | Biodosimetry for high dose accidental exposures by drug induced premature chromosome condensation (PCC) assay 显示文摘 | Sreedevi Balakrishnan kapil Shirsath Nagesh Bhat | 2010 | C Gytat Res2010，699，12： | 1 |
| 6 | Real-time RT-PCR detection and sequence analysis of the VP2 hypervariable region of Indian vvIBDV isolates 显示文摘 | Sreedevi B Jackwood D J | 2007 | Avian Dis2007，51，： | 1 |
| 7 | Efficiency of Women's Technical Institutions By Using BCC Model Through DEA Approach显示文摘 | Sreedevi IL P Pushpanjali K | 2013 | IOSIL Journal of mathematics2013，7，11： | 1 |
| 8 | Insights from principal component analysis applied to Py-GCMS study of Indian coals and their solvent extracted clean coal products显示文摘The present work aims at studying five Indian coals and their solvent extracted clean coal products using Py-GCMS analysis and correlating the characterization data using theoretical principal component analysis.The pyrolysis products of the original coals and the super clean coals were classified as mono-,di-and tri-aromatics,while other prominent products that were obtained included cycloalkanes,n-alkanes,and alkenes ranging from C_(10)-C_(29).The principal component analysis is a dimensionality reduction technique that reduced the number of input variables in the characterization dataset and gave inferences on the relative composition of constituent compounds and functional groups and structural insights based on scores and loading plots which were consistent with the experimental observations.ATR-FTIR studies confirmed the reduced concentration of ash in the super clean coals and the presence of aromatics.The Py-GCMS data and the ATR-FTIR spectra led to the conclusion that the super clean coals behaved similarly for both coking and noncoking coals with high aromatic concentrations as compared to the raw coal.Neyveli lignite super clean coal was found to show some structural similarity with the original coals,whereas the other super clean coals showed structural similarity within themselves but not with their original coal samples confirming the selective action of the e,N solvent in solubilizing the polycondensed aromatic structures in the coal samples. | Abyansh Roy Heena Dhawan Sreedevi Upadhyayula Hariprasad Kodamana | 2021 | International Journal of Coal Science & Technology2021，8，6： | 1 |
| 9 | Studies on naturallyoccurring infectious bursal disease viruses suggest that a single amino acidsubstitution at position 253 in VP2 increases pathogenicity显示文摘 | JACKWOOD D J SREEDEVI B LEFEVER L J | 2008 | Virology2008，377，： | 1 |
| 10 | Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro 显示文摘 | Sreedevi Nallamsetty | 2004 | Protein Expression and Purification2004，38，1： | 1 |
| 11 | Association of hy- drogeological factors in temporal variations of fluoride con- centration in a crystalline aquifer in India显示文摘 | Sreedevi P D Ahmed S Made B | 2006 | Environmental Geology2006，50，： | 1 |
| 12 | Performance comparison of capacitor-run induction motors supplied from AC voltage regulator and SPWM chopper显示文摘 | Sundareswaran K Rajasekar N Sreedevi V T | 2006 | IEEE Transactions on industrial Electronics2006，53，3： | 1 |
| 13 | Juvenile xanthogranuloma显示文摘 | Kesavan TM Sreedevi PK | 2005 | Indian Pediatrics2005，42，3： | 1 |
| 14 | Effect of temperature on development, survival and reproduction of the mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudoeoccidae ) on cotton 显示文摘 | Prasad YG Prabhakar M Sreedevi G | 2012 | Crop Protection2012，39，： | 1 |
| 15 | Characterization of infectious bursal disease viruses from four layer flocks in the United States 显示文摘 | Sreedevi B LeFever L J Sommer-Wagner S E | 2007 | Avian Dis2007，51，4： | 1 |
| 16 | Forecasting Groundwater level using artificial neural networks显示文摘 | Sreekanth P.D Geethanjali N Sreedevi P.D Shakeel Ahmed Ravikumar N Kamala Jayanthi P.D | | 0，，07： | 1 |
| 17 | Periodontal status in smokers and nonsmokers: A clinical, microbiological, and histopatholngical study显示文摘 | Sreedevi M Ramesh A Dwarakanath C | 2012 | Int J Dent2012，20，12： | 1 |
| 18 | Epidemiology of cervical cancer with special focus on India显示文摘 | Sreedevi A Javed R Dinesh A | 2015 | Int J Womens Health2015，7，： | 1 |
| 19 | Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity显示文摘 | Jackwood D J Sreedevi B LeFever L J | 2008 | Virology2008，377，： | 1 |
| 20 | Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity 显示文摘 | Jackwood D J Sreedevi B LeFever L J | 2008 | Virology2008，377，1： | 1 |
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