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| 1 | 用染色体畸变品系对果蝇调控基因E(ω~a)的定位显示文摘在果蝇中,杏黄色眼基因w^a和它的野生型白眼等位基因w的区别在于前者的转录单位中插入了反转录病毒样转座因子copia。半显性突变基因E(w^a)以反式方式降低w^a的活性。对多余翅脉基因px,E(w^a)和翅腋具黑点基因sp的遗传重组分析进一步确定了E(w^a)与sp的重组图距为0.2图距单位,并将E(w^a)定位于第二染色体右臂的2—106.8处。为了对E(w^a)进行细胞学的基因定位,4个Y;2易位品系,1个1;2易位品系和3个2R缺失品系与适当的E(w^a)品系进行杂交,并对其雄性子代进行复眼眼色和翅腋黑点的检查。结果表明E(w^a)位于第二染色体右臂60B的端粒端。 我们用r射线诱变获得了一个E(w^a)突变体和两个sp的突变体,并检查了它们的唾腺染色体。前者为E(w^a)的回复子,一段来源不明的染色体片段插入在6085—13的位置,很可能在60B8—9的位置上。这个位置就是E(w^a)的座位。后者在60C1—2的位置上都看到了染色体的断裂点,表明该位置为sp的基因座位。 综合细胞学基因定位和遗传重组基因定位的资料,E(w^a)被定位于6085—13的位置,很可能在60B8—9的位置,它位于sp基因的左侧,二者相距很近。 | 彭先步 Stephen M.Mount | 1993 | Acta Genetica Sinica1993，20，1： | 1 |
| 2 | Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences显示文摘The most popular CRISPR-SpCas9 systemrecognizes canonical NGG protospacer adjacent motifs(PAMs).Previously engineered SpCas9 variants,such as Cas9-NG,favor G-rich PAMs in genome editing.In this manuscript,we describe a new plant genome-editing system based on a hybrid iSpyMacCas9 platform that allows for targeted mutagenesis,C to T base editing,and A to G base editing at A-rich PAMs.This study fills amajor technology gap in the CRISPR-Cas9 system for editing NAAR PAMs in plants,which greatly expands the targeting scope of CRISPR-Cas9.Finally,our vector systems are fully compatible with Gateway cloning and will work with all existing single-guide RNA expression systems,facilitating easy adoption of the systems by others.We anticipate that more tools,such as prime editing,homology-directed repair,CRISPR interference,and CRISPR activation,will be further developed based on our promising iSpyMac-Cas9 platform. | Simon Sretenovic Desuo Yin Adam Levav Jeremy D.Selengut Stephen M.Mount Yiping Qi | 2021 | Plant Communications2021，2，2： | 1 |
| 3 | Rosaceae fruit transcriptome database(ROFT)-a useful genomic resource for comparing fruits of apple,peach,strawberry,and raspberry显示文摘Rosaceae is a large plant family consisting of many economically important fruit crops including peach,apple,pear,strawberry,raspberry,plum,and others.Investigations into their growth and development will promote both basic understanding and progress toward increasing fruit yield and quality.With the ever-increasing high-throughput sequencing data of Rosaceae,comparative studies are hindered by inconsistency of sample collection with regard to tissue,stage,growth conditions,and by vastly different handling of the data.Therefore,databases that enable easy access and effective utilization of directly comparable transcript data are highly desirable.Here,we describe a database for comparative analysis,ROsaceae Fruit Transcriptome database(ROFT),based on RNA-seq data generated from the same laboratory using similarly dissected and staged fruit tissues of four important Rosaceae fruit crops:apple,peach,strawberry,and red raspberry.Hence,the database is unique in allowing easy and robust comparisons among fruit gene expression across the four species.ROFT enables researchers to query orthologous genes and their expression patterns during different fruit developmental stages in the four species,identify tissue-specific and tissue-/stage-specific genes,visualize and compare ortholog expression in different fruit types,explore consensus co-expression networks,and download different data types.The database provides users access to vast amounts of RNA-seq data across the four economically important fruits,enables investigations of fruit type specification and evolution,and facilitates the selection of genes with critical roles in fruit development for further studies. | Muzi Li Stephen M.Mount Zhongchi Liu | 2023 | Horticulture Research2023，10，12： | 0 |
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