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您的检索式：作者名="Steven A Zinn"
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| 1 | Culture conditions for equine bone marrow mesenchymal stem cells and expression of key transcription factors during their differentiation into osteoblasts显示文摘Background:The use of equine bone marrow mesenchymal stem cells(BMSC) is a novel method to improve fracture healing in horses.However,additional research is needed to identify optimal culture conditions and to determine the mechanisms involved in regulating BMSC differentiation into osteoblasts.The objectives of the experiments were to determine:1) if autologous or commercial serum is better for proliferation and differentiation of equine BMSC into osteoblasts,and 2) the expression of key transcription factors during the differentiation of equine BMSC into osteoblasts.Equine BMSC were isolated from the sterna of 3 horses,treated with purchased fetal bovine serum(FBS) or autologous horse serum(HS),and cell proliferation determined.To induce osteoblast differentiation,cells were incubated with L-ascorbic acid-2-phosphate and glycerol-2-phosphate in the presence or absence of human bone morphogenetic protein2(BMP2),dexamethasone(DEX),or combination of the two.Alkaline phosphatase(ALP) activity,a marker of osteoblast differentiation,was determined by ELISA.Total RNA was isolated from differentiating BMSC between d 0 to 18 to determine expression of runt-related transcription factor2 {Runx2),osterix(Osx),and T-box3(Tbx3).Data were analyzed by ANOVA.Results:Relative to control,FBS and HS increased cell number(133 ±5 and 116 ± 5%,respectively;P < 0.001) and 5-bromo-2'-deoxyuridine(BrdU) incorporation(167±6 and 120±6%,respectively;P< 0.001).Treatment with DEX increased ALP activity compared with control(1,638 ±38%;P< 0.001).In the absence and presence of Dex,BMP-2 did not alter ALP activity(P>0.8).Runt-related transcription factor2 expression increased 3-fold(P< 0.001) by d 6 of culture.Osterix expression increased 9-fold(P<0.05) by d 18 of culture.Expression of Tbx3 increased 1.8-fold at d 3(P<0.01);however expression was reduced 4-fold at d 18(P<0.01).Conclusions:Dexamethasone,but not BMP-2,is required for differentiation of equine BMSC into osteoblasts.In addition,expression of Runx2 and osterix increased and expression of Tbx3 is reduced during differentiation. | Elizabeth R A Glynn Alfredo Sanchez Londono Steven A Zinn Thomas A Hoagl Kristen E Govoni | 2014 | Journal of Animal Science and Biotechnology2014，5，2： | 1 |
| 2 | Poor maternal nutrition inhibits muscle development in ovine offspring显示文摘Background:Maternal over and restricted nutrition has negative consequences on the muscle of offspring by reducing muscle fiber number and altering regulators of muscle growth.To determine if over and restricted maternal nutrition affected muscle growth and gene and protein expression in offspring,36 pregnant ewes were fed 60%,100%or 140%of National Research Council requirements from d 31 ± 1.3 of gestation until parturition.Lambs from control-fed(CON),restricted-fed(RES) or over-fed(OVER) ewes were necropsied within 1 d of birth(n = 18) or maintained on a control diet for 3 mo(n = 15).Semitendinosus muscle was collected for immunohistochemistry,and protein and gene expression analysis.Results:Compared with CON,muscle fiber cross-sectional area(CSA) increased in RES(58%) and OVER(47%)lambs at 1 d of age(P < 0.01);however at 3 mo,CSA decreased 15%and 17%compared with CON,respectively(P < 0.01).Compared with CON,muscle lipid content was increased in OVER(212.4%) and RES(92.5%) at d 1(P< 0.0001).Muscle lipid content was increased 36.1%in OVER and decreased 23.6%in RES compared with CON at 3mo(P< 0.0001).At d 1,myostatin mRNA abundance in whole muscle tended to be greater in OVER(P = 0.07) than CON.Follistatin mRNA abundance increased in OVER(P = 0.04) and tended to increase in RES(P = 0.06) compared with CON at d 1.However,there was no difference in myostatin or follistatin protein expression(P> 0.3).Phosphorylated Akt(ser473) was increased in RES at 3 mo compared with CON(P = 0.006).Conclusions:In conclusion,maternal over and restricted nutrient intake alters muscle lipid content and growth of offspring,possibly through altered gene and protein expression. | Sarah A Reed Joseline S Raja Maria L Hoffman Steven A Zinn Kristen E Govoni | 2015 | Journal of Animal Science and Biotechnology2015，6，1： | 0 |
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