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2篇 您的检索式:作者名="Su Guanmin"
    题名 作者 年代 出处 被引量
1Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent显示文摘Objective A highly sensitive and rapid high‐performance liquid chromatography method with pre‐column derivatization with 4‐fluoro‐7‐nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra‐ and the inter‐day coefficients of variation for the method were 5.3% and 7.7%,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma.WANG XiFeng CHI DeFeng SU GuanMin LI Lin SHAO LiHua 2011Biomedical and Environmental Sciences2011,24,5:13
2Improved determination of D-glucosamine hydrochloride in health foods by HPLC using 7-fluoro 4 nitrobenzo-2-oxa-1, 3-di- azole as a derivative显示文摘Su Guanmin Wang Xifeng Chi Defeng 2011ournal of Food Science2011,76,9:1
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