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| 1 | Differential mercury volatilization by tobacco organs expressing a modi-fied bacterial merA gene显示文摘Mercury pollution is a major environmental problem accompanying industrial activities. Most of the mercury released ends up and retained in the soil as complexes of the toxic ionic mercury (Hg2+), which then can be converted by microbes into the even more toxic methylmercury which tends to bioaccumulate. Mercury detoxification of the soil can also occur by microbes converting the ionic mercury into the least toxic metallic mercury (Hg0) form, which then evaporates. The remediation potentia1 of transgenic plants carrying the MerA gene from E. coLi encoding mercuric ion reductase could be evaluated. A modified version of the gene, optimized for plant codon preferences (merApe9, Rugh et al. 1996), was introduced into tobacco by Agrobacterium-mediated leaf disk transformation. Transgenic seeds were resistant to HgCl2 at 50 μM and some of them (10-20% ) could germinate on media containing as much as 350 μM HgCl2, while the control plants were fully inhibited or died on 50 μM HgCl2. The rate of elemental mercury evolution from Hg2+ (added as HgCl2) was 5-8 times higher for transgenic plants than the control. Mercury volatilization by isolated organs standardized for fresh weight was higher (up to 5 times) in the roots than in shoots or the leaves. The data suggest that it is the root system of the transgenic plants that volatilizes most of the reduced mercury (Hg0). It also suggests that much of the mercury need not enter the vascular system to be transported to the leaves for volatilization. Thansgenic plants with the merApe9 gene may be used to mercury detoxification for environmental improvement in mercury-- contaminated regions more efficiently than it had been predicted based on data on volatilization of whole plants via the upper parts only (Rugh et al. 1996). | HE YU KE, JIAN GE SUN, XIAN ZHONG FENG, MIHALY CZAKU, LASZLO MARTON Depariment of Biological Sciences, University of South Carolina, 700 Sumter Street, Columbia, SC29208, USA National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Phy | 2001 | Cell Research2001，11，3： | 14 |
| 2 | Tile HMG-I oncogene causes highly penetrant, aggressive lymphoid malignancy in transgenic mice and is overexpressed in human leukemia显示文摘 | Xu Y Sumter TF Bhattacharya R | 2004 | Cancer Res2004，64，10： | 1 |
| 3 | The HMG-I oncogene causes highly penetrant,aggressive lymphoid malignancy in transgenic mice and is overexpressed in human leukemia显示文摘 | Xu Y Sumter TF Bhattacharya R | | 0，，10： | 1 |
| 4 | The HMG-1 oncogene causes highly penetrant, aggressive lymphoid malignancy intransgenic mice and is overexpressed in human leukemia显示文摘 | Xu Y Sumter TF Bhattacharya R | 2004 | Cancer Res2004，64，10： | 1 |
| 5 | The developmental pattern of resistance to peer influence in adolescence:Will the teenager ever be able to resist显示文摘 | Sumter S.R Bokhorst C.L Steinberg L Westenberg P.M | | 0，，4： | 1 |
| 6 | Recombinant chicken and bovine growth hormone accelerate growth in aquaculturedjuvenile Pacific salmon ( Oncorhynchus kisutch) 显示文摘 | Gill J A Sumter J P Donaldson E M | 1985 | Bio Technology1985，3，： | 1 |
| 7 | The developmental pattern of resistance to peer influence in adolescence: will the teenager ever be able to resist? 显示文摘 | Sumter SR Bokhorst CL Steinberg L | 2009 | J Adolesc2009，32，4： | 1 |
| 8 | Bradykinin decreases podocyte permeability through ADAM17-dependent epidermal growth factor receptor activation and zonula occludens- 1 rearrangement显示文摘 | Dey M Baldys A Sumter DB | 2010 | J Pharmacol Exp Ther2010，334，3： | 1 |
| 9 | The HMG-Ⅰ oncogene causes highly penetrant,aggressive lymphoid malignancy intransgenic mice and is overexpressed in human leukemia显示文摘 | Xu Y Sumter TF Bhattacharya R | 2004 | Cancer Res2004，64，10： | 1 |
| 10 | The HMG-Ⅰ oncogene causes highly penetrant,aggressive lymphoid malignancy in transgenic mice and is overexpressed in human leukemia显示文摘 | XU Y SUMTER T F BHATTACHARYA R | 2004 | Cancer Res2004，64，10： | 1 |
| 11 | Bringing the field into the criminal justice classroom: Field trips, ride-alongs, and guest speakers显示文摘 | Payne B K Sumter M Sun I | 2003 | Journal of Criminal Justice Education2003，14，2： | 1 |
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