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| 1 | Proliferation of intestinal crypt cells by gastrin-induced ornithine decarboxylase显示文摘AIM:to determine whether the gastrin stimulated intestinalcrypt cell(IEC-6)proliferation by induction of ornithinedecarboxylase(ODC).METHODS:IEC-6 cells were grown in DMEM containing50mL·L^(-1)dialyzed fetal bovine serum for 24h and then weretreated with gastrin.The proliferative capability of the cellswas monitored subsequently on d 1,2,3,and 4 aftertreatment with MTT assay at aborbance 570nm.The cellularODC mRNA expression,ODC activity,and putrescinecontent were examined by RT-PCR method,radiometrictechnique and high-performance liquid chromatography(HPLC)analysis respectively after 12h of treatment.RESULTS:On d1 after exposure of IEC-6 cells topentagastrin,the proliferation increased initially and reacheda peak on d3 at 250μg·L^(-1)concentration.Pentagastrin 500μg·L^(-1)increased cell proliferation on day 1 and day 2,andthen decreased,Compared with control group,pentagastrin250μg·L^(-1)increased ODC mRNA level by 1.09-fold(P<0.05),ODC activity by 1.71-fold(P<0.0]),and putrescinecontent 5.30-fold(P<0.01)respectively.Similarly,pentagastrin of 500μg·L^(-1)also increased ODC mRNA levelby 1.16-fold(P<0.05),ODC activity 1.63-fold(P<0.05),and putrescine content 4.4t-fold(P<0.01)respectively.But there was not significant difference between them.CONCLUSION:Gastrin is an agent which promotes IEC-6 cellproliferation involved in regulating ODC activity mechanism. | Zi-Li Zhang Wei-Wen Chen Piei Institute,Guangzhou University of Traditional Chinese Medicine(TCM),Guangzhou 510405,China | 2002 | World Journal of Gastroenterology2002，8，1： | 11 |
| 2 | Harmine induces apoptosis in HepG2 cells via mitochondrial signaling pathway显示文摘BACKGROUND:Harmine has antitumor and antinociceptive effects,and inhibits human DNA topoisomerase.However no detailed data are available on the mechanisms of action of harmine in hepatocellular carcinoma.This study aimed to investigate the effects of harmine on proliferation and apoptosis and the underlying mechanisms in the human hepatocellular carcinoma cell line HepG2.METHODS:The proliferation of HepG2 cells was determined by the cell counting kit-8 (CCK-8) assay and the clone formation test.The morphology of HepG2 cells was examined using fluorescence microscopy after Hoechst 33258 staining Annexin V/propidium iodide (PI) was used to analyze apoptosis and PI to analyze the cell cycle.Western blotting was used to assess expression of the apoptosis-regulated genes Bcl-2,Bax,Bcl-xl,Mcl-1,caspase-3,and caspase-9 Mitochondrial transmembrane potential (Ψ m) was determined using JC-1.RESULTS:Harmine inhibited the proliferation of HepG2 cells in a dose-dependent manner.Hoechst 33258 staining revealed nuclear fragmentation and chromosomal condensation,cell shrinkage,and attachment loss in HepG2 cells treated with harmine.The percentage of the sub/G1 fraction was increased in a concentration-dependent manner,indicating apoptotic cell death.PI staining showed that harmine changed the cell cycle distribution,by decreasing the proportion of cells inG0/G1 and increasing the proportion in S and G2/M.Harmine induced apoptosis in a concentration-dependent manner,with rates of 20.0%,32.7% and 64.9%,respectively.JC-1 revealed a decrease in Ψ m.Apoptosis of HepG2 cells was associated with caspase-3 and caspase-9 activation,down-regulation of Bcl-2,Mcl-1,and Bcl-xl,and no change in Bax.CONCLUSIONS:Harmine had an anti-proliferative effect in HepG2 cells by inducing apoptosis.Mitochondrial signal pathways were involved in the apoptosis.The cancer-specific selectivity shown in this study suggested that harmine is a promising novel drug for human hepatocellular carcinoma. | Ming-Rong Cao,Qiang Li,Zhi-Long Liu,Hui-Hui Liu,Wei Wang,Xiao-Li Liao,Yun-Long Pan and Jian-Wei Jiang Department of General Surgery,First Affiliated Hospital,Jinan University,Guangzhou 510632,China Department of Anesthesiology,First Affiliated Hospital,Guangzhou University of TCM,Guangzhou 510632,China Department of Biochemistry,Medical College of Jinan University,Guangzhou 510632,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，6： | 11 |
| 3 | Influence of BOL on hyaluronic acid,laminin and hyperplasia in hepatofibrotic rats显示文摘AIM: To study the anti-hepatofibrosis mechanism of Bie Jie Jian oral liquid (BOL).METHODS: The model was induced by subcutaneous injection of CCl4. BOL was administered and the change of serum hyaluronic acid (HA) and laminin (LN) was observed and the degeneration of liver cells and the degree of fibre hyperplasia analyzed. Changes of ultra micro-structure in liver cells were observed in some samples.RESULTS: HA was reduced in both the groups with Iow and high dosage of BOL, which showed a remarkable difference as compared with that of the model group ( Iow dosage group: 376.15 μg/L ± 35.48 μg/L vs 806.07 μg/L ± 98.49 μg/L, P < 0.05; high dosage group: 340.14 μg/L ± 30.18 μg/L vs 806.07μg/L± 98.49 μg/L, P<0.05). The LN content of Iow and high dosage group of BOL was lower than that of model group (Iow dosage group: 71.99 μg/L± 8.15 μg/L vs 133.94μg/L± 14.45 μg/L, P< 0.01; high dosage group: 71.68 ig/L± 11.62 μg/L vs 133.94 μg/L ± 14.45 μg/L, P<0.01) and colchicine group (Iow dosage group: 71.99 μg/L ± 8.15 μg/Lvs 118.28 μg/L ± 16.13 μg/L, P< 0.05; high dosage group:71.68 μg/L ± 11.62 μg/L vs 118.28 μg/L ± 16.13 μg/L,P<0.05). Examined by Ridit, BOL could reduce the degeneration and necrosis of liver cells (X2 = 11.99P<0.05), the degree of fibre hyperplasia (X2 = 13.24P<0.05) and the pathological change of ultra micro-structure as well.CONCLUSION: The BOL has certain therapeutic effect on the experiment hepatofibrosis. Its mechanisms might include:protecting the function of liver cells, inhibiting excessive synthesis and secretion of extraceiluar matrix from hepatic stellate cells, relieving the cepillarization of hepatic sinueoid, improving liver micro-circulation, and regulating immune function. | Li Yao~1 Zhen-Min Yao~2 Tao Yu~2 1 Pharmacology Department of Zhejiang College of Traditional Chinese Medicine(TCM),Hangzhou 310053,Zhejiang Province,China2 Basic Medicine Department of Zhejiang College of TCM,Hangzhou 310053,Zhejiang Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 9 |
| 4 | Jiang Tang Fang Long Jiao Nang in Treating Non-Insulin Dependent Diabetes Mellitus with Hearing Loss显示文摘Objective To study the efficacy and safety of the Jiang Tang Fang Long Jiao Nang (Hypoglycemic Anti-Deafness Capsule) in treating non-insulin dependent diabetes mellitus with hearing loss. Methods Two hundred ninety six patients with non-insulin dependent diabetes mellitus and hearing loss were randomly assigned to a treatment group(n=164, 208 ears) and a control group(n=132, 184 ears). Patients in the treatment group were treated with the Jiang Tang Fang Long Jiao Nang and supplement herbal preparations as indicated by traditional Chinese medicine dialectical assessment, while control patients received glibenclamide and conventional treatments for deafness. Hearing, fasting blood glucose(FBG), post-prandial blood glucose(PBG), 24 hour urine sugar, platelet function indices, blood superoxide dismutase(SOD) and lipid peroxides(LPO) levels, and symptom improvement were compared between the two groups. Results The rate of hearing improvement was 56.7% for the treatment group and 26.6% for the control. FBG, PBG and 24 hour urine sugar improved in both groups, but the last two were superior in the treatment group compared to the control. Symptoms improvement was also superior in the treatment group compared to the control. In patients receiving Jiang Tang Fang Long Jiao Nang treatment, platelet function indices, SOD and LPO were all improved, while only LOP improvement was noticed in control patients. No acute or long-term toxicity was demonstrated for the Jiang Tang Fang Long Jiao Nang in animal tests. The Jiang Tang Fang Long Jiao Nang lowered blood glucose and serum triglycerides in a rat model of alloxan-induced diabetes. Conclusion The Jiang Tang Fang Long Jiao Nang is effective in improving hearing and diabetic indices in diabetic patients with deafness, without significant side effects. | LI Rui-yu1, LI Meng2, WANG Rui3, LI Bin4, GUO Ya-jing5 1 Xingtai Medical College, Hebei, Xingtai, 054000 2 Chengde Medical college Hebei, Chengde, 067000 3 College of Traditional Chinese Medicine, Hebei Medical University, Shijiazhuang, 050091 4 Department of Biochemistry, Hebei Medicine University 050017 5 Guangzong Hospital of TCM, Hebei, 054600 | 2008 | Journal of Otology2008，3，1： | 7 |
| 5 | 中风病始发态证候影响因素的研究显示文摘调查分析性别、年龄、职业、伴发疾病、既往史对1663例中风病始发态患者证候得分均值和发生概率的影响。结果显示:男性疾湿证显著高于女性;年龄对证候有显著影响,并与气虚证、痰湿证、血瘀证呈显著正相关关系;职业对证候无规律性影响;伴发疾病评分与风证、火热证、痰湿证、气虚证、阴虚阳亢证里显著正相关关系。提示临床辨证、证候研究、新药研制中要重视和考虑上述因素的影响。 | Wang Shundao Ren Zhanli Du Menghua et, al.(Xingtai Municipal people’s Hospital, Hebei 054031)(Beijing University of TCM, Beijing 100029) | 1996 | 北京中医药大学学报1996，19，4： | 6 |
| 6 | Simultaneous Analysis of Thirteen Bioactive Components in Evodia rutaecarpa and Its Varieties by HPLC-DAD-MS显示文摘Objective To control the quality of Evodia rutaecarpa better.Methods An HPLC-DAD-MS/MS method was established for the rapid and efficient identification of bioactive constituents and for simultaneous quantitative analysis of four bioactive ingredients including evodiamine,rutaecarpine,dehydroevodiamine,and evodin in E.rutaecarpa,which was applied to evaluating eight samples of E.rutaecarpa and its varieties from different areas.Results Thirteen potentially bioactive constituents including one flavonoid glycoside,one limonin,four indoloquinazoline alkaloids,and seven quinolone alkaloids were identified in all samples and the contents of dehydroevodiamine,evodine,evodiamine,and rutaecarpine varied widely from 0.10% to 0.51%,0.49% to 3.12%,0.07% to 1.56%,and 0.10% to 0.69%,respectively.Conclusion This method is found to be convenient,fast,accurate,and it is facilitated to improve the quality control standard of E.rutaecarpa and related products. | XU Hai-yu1,3,ZHANG Tie-jun2,XIAO Xue-feng1,ZHAO Ping1,LIU Chang-xiao3,XU Jun2 1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin Engineering Laboratory of Quality Control Techniques for TCM,Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China 3.The National Laboratory of Pharmacodynamics and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China | 2010 | Chinese Herbal Medicines2010，2，2： | 6 |
| 7 | THE EXTENSIVENESS AND SPECIFICITY OF EFFECT OF ELECTROACUPUNCTURE AT DIFFERENT ACUPOINTS ON NOCICEPTIVE RESPONSE OF CONVERGENT NEURONS IN TRIGEMINAL NUCLEUS CAUDALIS显示文摘Experiments were carried out on rats anaesthetized with uraethane. The sponta-neous discharges and nociceptive responses of convergent neurons in the right trigerninal nucleus cau-dalis(TNC) to noxious stimuli at receptive field (cheek) were recorded extracellularly with glass mi-cro-electrode. Electroacupuncture (EA ) was applied at bilateral ' Xiaguan' (ST 7 on face ) or 'Zusanli' (ST 36 on shank) acupoint with Iow (2V) and high (18V) intensity. The noclceptive re-sponse of convergent neurons in TNC could be inhihited by low intensity EA applied at 'Xiaguan' butnot 'Zusanlil', showing the specificity of acupoints. High intensity EA at either 'Xiaguan' or 'Zusan-li' also reduced the nociceptive responses, showing the analgesic extensiveness of acupoints. We sug-gest that 'the gate of control' mechanism plays a main role in low intensity EA and 'diffuse noxiousinhibitory controls' (DNIC) rnechanism does so in high intensity EA.The results suggest that we should pay attention to the location of acupoints, | Xu Weidong, Liu Xiang. Zhu Bing. He Xiaoling. Zhang ShouxinInstitute of Acupuncture and Moxibustion, China Academy of TCM Beijing 100700, China | 1995 | World Journal of Acupuncture-Moxibustion1995，5，2： | 5 |
| 8 | EXPERIMENTAL WORK AND RESEARCH The Experimental Study of Xue Guan Tong(血管通)on PreventingRestenosis after Percutaneous Transluminal A显示文摘EXPERIMENTALWORKANDRESEAROHTheExperimentalStudyofXueGuanTong(血管通)onPreventingRestenosisafterPercutaneousTransluminalAngioplas... | Shi Da-zhuo (史大卓) Xu Feng-ning (徐凤芹) Li Li-zhi (李立志) Ma Xiacachang (马晓昌) Wang Xiao-fang (汪晓芳) and Chen Ke-ji (陈可冀)(Hi-yuan Hospital, China Academy of TCM, Beijing 100091 )Li Ping (李平) Mao Jie-ming(毛节明) Chen Ming-zhe (陈明哲)an | 1995 | Chinese Journal of Integrative Medicine1995，1，4： | 3 |
| 9 | THE INFLUENCE OF ELECTRO-ACUPUNCTURE AT NEIGUAN AND FIVE SHU POINTS OF TWELVE REGULAR MERIDIANS AT DIFFERENT TIME ON SYSTOLIC TIME INTERVAL OF NORMAL YOUNG ADULTS显示文摘The influence of electro-acupuncture at Neiguan (PC 6)and five shu points of twelve regular meridians at different time on systolic time interval (ST I) of normal young adultswere studied with 6×6 Latin square design in this work.In 12 groups according to 12 regular merldlans,the STIs were measured respectively just before and after electro-acupuncture on Neiguan andfive shu points at 00:00,04:00,08:00,12:00,16:00 and 20:00 of a day.It was found thatexcept the PEPI and PEPI/LVETI ratio among Shenmen(HT 7),Shaochong(HT 9)and Neiguan,Guanchong(TE)and Yemen(TE 2)points,the effects of electro-acupuncture at Neiguan pointand five shu points of twelve regular meridians on the indices of STI of normal young adults wereshowing no slgnificant differences.There also was no difference in different sex.But the indices ofSTI were changed when electroacupuncture on the different points of different meridians at differenttime. | Li Lei, Chen Hanping, Xi Yongjiang, Gu JieShanghai University of TCM, Shanghai 200032, China | 1994 | World Journal of Acupuncture-Moxibustion1994，4，1： | 2 |
| 10 | Hemsleyasine, a New Norditerpenoid Alkaloid from the Roots of Aconitum Hemsleyanum Var.Circinatum显示文摘A new norditerpenoid alkaloid, hemsleyasine 1, has been isolated from the roots of Aconitum hemsleyanum var. circinatum W. T. Wang and its structure was established by spectral methods (1 H- and 13C-NMR. 1H COSY, COLOC and nOes). | Xu, QY Wang, FP Tan, TCM Che, CT | 1997 | Chinese Chemical Letters1997，8，2： | 2 |
| 11 | Malereproductivesystem structure and accessory glandsultrastructure of species of Triatoma (Hemiptera, Reduviidae,Triatominae) 显示文摘 | Freitas SPC Goncalves TCM Serrao JE | 2010 | Micron2010，41，5： | 1 |
| 12 | VENTRICAL MICROINJECTING ATROPINE OR NALOXONE REDUCES ANALGESIC EFFECTS PRODUCED BY BRAIN STIMULATION IN THE RAT显示文摘Stimulating SmI cortex like needling points produced analgesic effect in rats.Under the background of ventrical microinjecting atropine(10μg/2μl)or naloxone(20μg/20μl)tailflick latency(TEL)remained unchanged after stimulating SmI.Comparing atropine group or naloxone group with normal saline group it was shown that there were a statistical difference in TFL between the two groups respectively.Thus,both ACh and endogenous morphine-like factors may participate in analgesic effect as a neurotransmitter of the corticofugal modulation of pain. | Xu Wei, Yan Yasheng, Shi Hong, Chen ZhengqiuInstitute of Acupuncture, China Academy of TCM, Beijing 100700, China | 1994 | World Journal of Acupuncture-Moxibustion1994，4，4： | 1 |
| 13 | Cliamtic change and hydroph te--dom inated communities in inland wetland eco system 显示文摘 | Brock Tcm VierssanW V | | Wetland Ecology and Management0，1992，2： | 1 |
| 14 | High dielectricVDF/TrFE/CTFE terpolymers prepared by hydrogenation ofVDF/CTFE copolymers: Synthesis and characterization 显示文摘 | Wang ZM Zhang ZC Chung TCM | 2006 | Macromolecules2006，39，13： | 1 |
| 15 | OBSERVATIONS ON THE CLINICAL EFFECT AND NAIL-FOLD MICROCIRCULATION IN THE TREATMENT OF 42 CASES OF CERVICAL SPONDYLOPATHY BY ACUPUNCTURE AND MASSAGE显示文摘Forty-two cases of cervical spondylopathy were divided into acupuncture andmassage groups. The therapeutic effects were observed comparatively, and the changes of nail-foldmicrocirculation of patients before and after treatment were observed at the same time. The resultsshowed that the effective rates of two groups were all 100%, no difference between them. The nailfold microcirculation of two groups was markedly ameliorated after treatment, and no significant dif-ference between them either. | Wang Xuezhong Wang Fengqin, Dept. of Acupuncture and Moxibustion, Peking Union Hospital, Beijing 100730, ChinaWang Yan Tao Lihua, Laboratory of TCM, Peking Union Hospital, Beijing 100730, China | 1993 | World Journal of Acupuncture-Moxibustion1993，3，1： | 1 |
| 16 | THE CO-RELATIONSHIP BETWEEN NEIGUAN POINT AND THE HEART AND REGULATION OF AMYGDALOID NUCLEI显示文摘In this present study, the ruodel of the rabbit with acute myocardial ischemia(AMI) was used to explore mechanisms of interrelation hetween Neiguan acupoint (PC 6) of the Peri-cardium Meridian and the heart hy employing physiological and morphologicaI methods. It wasdemonstrated that electroacupuncture (EA) at Neiguan (PC 6 ) couId raise the eIectrical excitahility ofischemic myocardium, lessen the dispersity of recovery excitability, correct the disorder of electricalactivity; protect myocardiac glycogen and phosphorylase and alleviate their depletion, increase contentof ribonuclei acid (RNA ), etc., indicating that EA could help oxidative rnetaholism carry out normal-Iy and irnProve nutritional state of the ischemic myocardiurn. The key point of EA action is probablyregulates the coronary microclrculation of heart and reclistributes myocardial blood flow. Studies withalkaline phosphatase (ALP ), Mg2--ATPase and ABS casting of the Ieft coronary artery showed thatafter EA the number and the Iength | Cao Qingshu, Wen Shen, Liu Junling, Han Zhenjing, Chen ShupingInstitute of Acupuncture and Moxibustion , China Academy of TCM, Beijing 100700 , China | 1995 | World Journal of Acupuncture-Moxibustion1995，5，2： | 1 |
| 17 | Impact of a benzoyl urea insecticide on aquatic macroinvertebrates in ditch mesocosms with and without non-sprayed sections显示文摘 | Brock TCM Roessink I Belgers JDM | 2009 | Environmental toxicology and chemistry2009，28，21： | 1 |
| 18 | Conservative Therapy for Low Back Pain due to Lumbar Disc Herniation:A Randomized Control ed Clinical Trial显示文摘 | Yuan WA Huang SR Guo K Integrative TCM | | 0，，： | 1 |
| 19 | THE ULTRASTRUCTURE AND ENZYME HISTOCHEMISTRY OF THE SUBSTANTIA GELATINOSA OF RAT SPINAL CORD UNDER THE INFLUENCE OF ELECTRO-ACUPUNCTURE ANALGESIA显示文摘Histochemistry and EM were used in this experiment to study the effect of EAon the spinal dorsal horn of rat. After 30 minutes EA, the pain threshold of EA rats were elevated400%, the acid phosphatase reaction in spinal dorsal horn of EA rats increased obviously, the roundagranular vesicles of the C-terminals of EA group was more than that of control’s. The results sug-gested that the histochemical components and numbers of synaptic vesicles in SG and their functionscould be regulated by acupuncture. | Pan Baohan Ge Zi, Institute of Acupuncture and Moxibustion, China Academy of TCM, Beijing 100700, China | 1993 | World Journal of Acupuncture-Moxibustion1993，3，1： | 1 |
| 20 | Brain necrosis after radiotherapy for primary intracerebral tumor显示文摘 | Hohwieler ML Lo TCM Silverman ML | 1992 | Neurosurgery1992，18，： | 1 |
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