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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | Model combining pre-transplant tumor biomarkers and tumor size shows more utility in predicting hepatocellular carcinoma recurrence and survival than the BALAD models显示文摘AIM To assess the performance of BALAD, BALAD-2 and their component biomarkers in predicting outcome of hepatocellular carcinoma(HCC) patients after liver transplant.METHODS BALAD score and BALAD-2 class are derived from bilirubin, albumin, alpha-fetoprotein(AFP), Lens culinaris agglutinin-reactive AFP(AFP-L3), and des-gammacarboxyprothrombin(DCP). Pre-transplant AFP, AFP-L3 and DCP were measured in 113 patients transplanted for HCC from 2000 to 2008. Hazard ratios(HR) for recurrence and death were calculated. Univariate and multivariate regression analyses were conducted. C-statistics were used to compare biomarker-based to predictive models. RESULTS During a median follow-up of 12.2 years, 38 patients recurred and 87 died. The HRs for recurrence in patients with elevated AFP, AFP-L3, and DCP defined by BALAD cut-off values were 2.42(1.18-5.00), 1.86(0.98-3.52), and 2.83(1.42-5.61), respectively. For BALAD, the HRs for recurrence and death per unit increased score were 1.48(1.15-1.91) and 1.59(1.28-1.97). For BALAD-2, the HRs for recurrence and death per unit increased class were 1.45(1.06-1.98) and 1.38(1.09-1.76). For recurrence prediction, the combination of three biomarkers had the highest c-statistic of 0.66 vs. 0.64, 0.61, 0.53, and 0.53 for BALAD, BALAD-2, Milan, and UCSF, respectively. Similarly, for death prediction, the combination of three biomarkers had the highest c-statistic of 0.66 vs 0.65,0.61, 0.52, and 0.50 for BALAD, BALAD-2, Milan, and UCSF. A new model combining biomarkers with tumor size at the time of transplant(S-LAD) demonstrated the highest predictive capability with c-statistics of 0.71 and 0.69 for recurrence and death. CONCLUSION BALAD and BALAD-2 are valid in transplant HCC patients, but less predictive than the three biomarkers in combination or the three biomarkers in combination with maximal tumor diameter(S-LAD). | Nicha Wongjarupong Gabriela M Negron-Ocasio Roongruedee Chaiteerakij Benyam D Addissie Essa A Mohamed Kristin C Mara William S Harmsen J Paul Theobald Brian E Peters Joseph G Balsanek Melissa M Ward Nasra H Giama Sudhakar K Venkatesh Denise M Harnois Michael R Charlton Hiroyuki Yamada Alicia Algeciras-Schimnich Melissa R Snyder Terry M Therneau Lewis R Roberts | 2018 | World Journal of Gastroenterology2018，24，12： | 5 |
| 3 | Dysfunctional stem and progenitor cells impair fracture healing with age显示文摘Successful fracture healing requires the simultaneous regeneration of both the bone and vasculature;mesenchymal stem cells (MSCs) are directed to replace the bone tissue, while endothelial progenitor cells (EPCs) form the new vasculature that supplies blood to the fracture site. In the elderly, the healing process is slowed, partly due to decreased regenerative function of these stem and progenitor cells. MSCs from older individuals are impaired with regard to cell number, proliferative capacity, ability to migrate, and osteochondrogenic differentiation potential. The proliferation, migration and function of EPCs are also compromised with advanced age. Although the reasons for cellular dysfunction with age are complex and multidimensional, reduced expression of growth factors, accumulation of oxidative damage from reactive oxygen species, and altered signaling of the Sirtuin-1 pathway are contributing factors to aging at the cellular level of both MSCs and EPCs. Because of these geriatric-specific issues, effective treatment for fracture repair may require new therapeutic techniques to restore cellular function. Some suggested directions for potential treatments include cellular therapies, pharmacological agents, treatments targeting age-related molecular mechanisms, and physical therapeutics. Advanced age is the primary risk factor for a fracture, due to the low bone mass and inferior bone quality associated with aging;a better understanding of the dysfunctional behavior of the aging cell will provide a foundation for new treatments to decrease healing time and reduce the development of complications during the extended recovery from fracture healing in the elderly. | Diane R Wagner Sonali Karnik Zachary J Gunderson Jeffery J Nielsen Alanna Fennimore Hunter J Promer Jonathan W Lowery M Terry Loghmani Philip S Low Todd O McKinley Melissa A Kacena Matthias Clauss Jiliang Li | 2019 | World Journal of Stem Cells2019，11，6： | 3 |
| 4 | 14 Years of Eosinophilic Esophagitis: Clinical Features and Prognosis显示文摘 | Jonathan M Spergel Terri F Brown-Whitehorn Janet L Beausoleil James Franciosi Michele Shuker Ritu Verma Chris A Liacouras | 2009 | Journal of Pediatric Gastroenterology and Nutrition2009，，1： | 2 |
| 5 | Monocyte-derived Wnt5a regulates inflammatory ymphangiogenesis显示文摘 | Roberto Sessa Don Yuen Stephanie Wan Michael Rosner Preethi Padmanaban Shaokui Ge April Smith Russell Fletcher Ariane Baudhuin-Kessel Terry P Yamaguchi Richard A Lang LuChen | 2016 | Cell Research2016，26，2： | 2 |
| 6 | 1-Methylcyclopropene treatment affects strawberry fruit decay显示文摘 | Yueming Jiang Daryl C Joyce Leon A Terry | 2001 | Postharvest Biology and Technology2001，，3： | 2 |
| 7 | Thips pollination of the central Australian cycad,Macrozamia macdonnellii(Cycadales)显示文摘 | Mound L A Terry I | 2001 | Inter J Plant Sci2001，162，： | 1 |
| 8 | Proviral insertions induce the expression of bone-specific isoforms of PEBP2alphaA (CBFA1):evidence for a new myc collaborating oncogene显示文摘 | Stewart M Terry A Hu M | 1997 | Proc Natl Acad Sci USA1997，94，16： | 1 |
| 9 | A two-stage technique for the digestion of forage crops显示文摘 | Tilley J M A Terry R A | 1963 | Br Grassl Soc1963，18，： | 1 |
| 10 | Marsh,Niklas Wagner,Return-volume dependence and extremes in international equity markets显示文摘 | Terry A | 2000 | working paper RPF-293 Haas school of business UC Berkeley2000，2000，： | 1 |
| 11 | Cadmium tolerance and accumulation in Indian mustard is enhanced by overexpressing- glutamylcysteine synthetase显示文摘 | ZHU Y PILON-SMITS E A H TERRY N | 1999 | Plant Physiol1999，121，4： | 1 |
| 12 | Experimental investigation of drying shrinkage and creep of concrete using fibre-optic sensors 显示文摘 | Wong A C L Childs P A Terry W | 2007 | Advances in Structural Engineering2007，10，3： | 1 |
| 13 | Marine fouling and its effects on off shore structures in the North Sea-a review 显示文摘 | Edyvean R G Terry L A Picken G B | 1985 | In- teraational Biodeterioration1985，21，4： | 1 |
| 14 | Effect of tumor necrosis fac- tor-a and interleukin-1α on heme oxygenasel-1 expression in human endothelial cells显示文摘 | Terry CM Jennifer A Clickeman JR | 1998 | Am J Physiol1998，274，： | 1 |
| 15 | Supercritical fluid extraction of polychlorinated dibenzo-p-dioxins from municipal incinerator fly ash显示文摘 | ONUSKA F I TERRY K A | 1991 | Journal of High Resolute Chromatography1991，14，： | 1 |
| 16 | A meta-analysis of relations between person–organization fit and work attitudes显示文摘 | Michelle L Verquer Terry A Beehr Stephen H Wagner | 2002 | Journal of Vocational Behavior2002，，3： | 1 |
| 17 | Biocontrol in an impulsive predator - prey model 显示文摘 | Terry A J | 2014 | Mathematical Biosciences2014，256，： | 1 |
| 18 | Supply chain coordination under channel rebates with sales effort effects 显示文摘 | Terry A Taylor | 2002 | Management Science2002，48，8： | 1 |
| 19 | Development of Normative Date for the Profile of Mood States for Use with Athletic Samples 显示文摘 | TERRY P C LANE A M | 2000 | JAppl Sport Psycho12000，12，： | 1 |
| 20 | A case study assessment of the operational performance of a multiple fresh produce distribution centre in the UK 显示文摘 | Ioannis Manikas Leon A Terry | 2009 | British Food Journal2009，111，5： | 1 |
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