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| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | An integrated approach for increasing breeding efficiency in apple and peach in Europe显示文摘Despite the availability of whole genome sequences of apple and peach,there has been a considerable gap between genomics and breeding.To bridge the gap,the European Union funded the FruitBreedomics project(March 2011 to August 2015)involving 28 research institutes and private companies.Three complementary approaches were pursued:(i)tool and software development,(ii)deciphering genetic control of main horticultural traits taking into account allelic diversity and(iii)developing plant materials,tools and methodologies for breeders.Decisive breakthroughs were made including the making available of ready-to-go DNA diagnostic tests for Marker Assisted Breeding,development of new,dense SNP arrays in apple and peach,new phenotypic methods for some complex traits,software for gene/QTL discovery on breeding germplasm via Pedigree Based Analysis(PBA).This resulted in the discovery of highly predictive molecular markers for traits of horticultural interest via PBA and via Genome Wide Association Studies(GWAS)on several European genebank collections.FruitBreedomics also developed pre-breeding plant materials in which multiple sources of resistance were pyramided and software that can support breeders in their selection activities.Through FruitBreedomics,significant progresses were made in the field of apple and peach breeding,genetics,genomics and bioinformatics of which advantage will be made by breeders,germplasm curators and scientists.A major part of the data collected during the project has been stored in the FruitBreedomics database and has been made available to the public.This review covers the scientific discoveries made in this major endeavour,and perspective in the apple and peach breeding and genomics in Europe and beyond. | Francois Laurens Maria JoséAranzana Pere Arus Daniele Bassi Marco Bink Joan Bonany Andrea Caprera Luca Corelli-Grappadelli Evelyne Costes Charles-Eric Durel Jehan-Baptiste Mauroux Hélène Muranty Nelson Nazzicari Thierry Pascal Andrea Patocchi Andreas Peil Bénédicte Quilot-Turion Laura Rossini Alessandra Stella Michela Troggio Riccardo Velasco Eric van de Weg | 2018 | Horticulture Research2018，5，1： | 10 |
| 3 | Response of soil mite abundance and diversity to a monospecific timber Tectona grandis plantation in Ivory Coast显示文摘 | Julien Kouadio N'DRI Henri Marc ANDRE Jan LAGERLOEF Jerome Ebagnerin TONDOH Thierry HANCE | 2013 | Current Zoology2013，59，5： | 3 |
| 4 | Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach显示文摘 | Zhijun Shen Carole Confolent Patrick Lambert Jean-Luc Po?ssel Bénédicte Quilot-Turion Mingliang Yu Ruijuan Ma Thierry Pascal | 2013 | Tree Genetics & Genomes2013，，6： | 2 |
| 5 | Enhanced serotonin transporter function during depression in seasonal affective disorder显示文摘 | Willeit M Sitte HH Thierry N | 2008 | Neuropsychopharmacology2008，33，10： | 1 |
| 6 | Evaluation of a DNA microarray (check-MDR CT102) for rapid detection of TEM,SHV, and CTX-M extended-spectrum 13-1actamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapene- mases显示文摘 | Thierry N GaeIle C Pierre B | 2011 | Clin Microbiol2011，49，4： | 1 |
| 7 | Achievable throughout optimization in OFDM systems in the presence of interference and its application to power line networks 显示文摘 | Thanh N V Karlne A Thierry C Pierre S | 2014 | IEEE Transactions on Communications2014，62，5： | 1 |
| 8 | Molecular mechanisms of the cardiovascular protective effects of polyphenols显示文摘 | Andriantsitohaina R Auger C Thierry Chataigneau T ltienne-Selloum N Huige Li H Marttnez MC Schini-Kerth VB Laher I | | 0，，： | 1 |
| 9 | Strategic Issues in Product Recovery Management显示文摘 | Thierry M Salomon M Van N J | 1995 | California Management Review1995，37，2： | 1 |
| 10 | An Automatic Method for Vine Detection in Airborne Imagery Using Wavelet Transformation and Multi -resolution Analysis 显示文摘 | THIERRY CATER BERNARD N | 2001 | Photo Grammetric Engineering& Remote Sensing2001，71，6： | 1 |
| 11 | Cooperative subcontracting relationship within a project supply chain: a simulation approach显示文摘 | PARROD N THIERRY C FANRGIER H | 2007 | Simulation Modelling Practice and Theory2007，15，1： | 1 |
| 12 | SHV-type extendedspectrumβ-lactamasc in a shigella flexneri clinical isolate显示文摘 | Nicolas F Thierry N Olivier G | 2001 | J Antimicrob Chemoth2001，47，： | 1 |
| 13 | Novel genentic structure associated with an extended-spectrum β-lactamase blaVEB gene in a Providencia stuartii clinical isolate from Algeria显示文摘 | Dainel A Thierry N Marie FL | 2005 | J Antimi crob Agents Chemother2005，49，8： | 1 |
| 14 | Strategic issues in product recovery management 显示文摘 | Thierry M C Salomon M Van Nunen J A E E Van Wassenhove L N | 1995 | California Management Review1995，37，2： | 1 |
| 15 | Enhanced serotonin tr-ansporter funotion during depression in seasonal affective dis-order 显示文摘 | Willeit M Sitte HH Thierry N | 2008 | Neuropsychopharmacology2008，33，7： | 1 |
| 16 | Direct numerical simulation of heat release and NOx formation in turbulent nonpremixed flames显示文摘 | Benoit Bedat Fokion N Egolfopoulos Thierry Poinsot | 1999 | Combustion and Flame1999，119，： | 1 |
| 17 | Phenolic profiles and an- tioxidative effects of hawthorn cell suspensions, fresh fruits, and medici- nal dried parts显示文摘 | THOMAS F THIERRY H FRANCOISE M N | 2009 | Food Chem2009，115，3： | 1 |
| 18 | The influence of microstructure on the corrosion behavior of AZ91D studies by scanning Kelvin probe force microscopy and scanning Kelvin probe 显示文摘 | JONSSON M THIERRY D BOZEC N L | 2006 | Corrosion Science2006，48，： | 1 |
| 19 | Corrosion product formation on Zn55Al coated steel upon exposure in a marine atmosphere 显示文摘 | PERSSON D THIERRY D LEBOZEC N | 2011 | Corrosion Science2011，53，： | 1 |
| 20 | Contactless power and information transmission显示文摘 | THIERRY B MARC P VALERIE N | 2002 | IEEE Trans on Industry Applications2002，38，5： | 1 |
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