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| 1 | Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81显示文摘AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs. | Zhan-Fei Tian Hong Shen Xi-Hua Fu Yi-Chun Chen Hubert E Blum Thomas F Baumert Xi-Ping Zhao | 2009 | World Journal of Gastroenterology2009，15，2： | 16 |
| 2 | Should antibiotics be administered before arthroscopic knee surgery? A systematic review of the literature显示文摘AIM To explore the current evidence surrounding the administration of prophylactic antibiotics for arthroscopic knee surgery. METHODS Databases were searched from inception through May of 2018 for studies examining prophylactic antibiotic use and efficacy in knee arthroscopy. Studies with patient data were further assessed for types of arthroscopic procedures performed,number of patients in the study,use of antibiotics,and outcomes with the intention of performing a pooled analysis. Data pertaining to 'deep tissue infection' or 'septic arthritis' were included in our analysis. Reported data on superficial infection were not included in our data analysis. For the pooled analysis,a relative risk ratio was calculated and χ~2 tests were used to assess for statistical significance between rates of infection amongst the various patient groups. Post hoc power analyses were performed to compute the statistical power obtained from our sample sizes. Number needed to treat analyses were performed for statistically significant differences by dividing 1 by the difference between the infection rates of the antibiotic and no antibiotic groups. An alpha value of 0.05 was used for our analysis. Study heterogeneity was assessed by Cochrane's Q test as well as calculation of the I^2 value.RESULTS A total of 49682 patients who underwent knee ar-throscopy for a diverse set of procedures across 19 studies met inclusion critera for pooled analysis. For those not undergoing graft procedures,there were 27 cases of post-operative septic arthritis in 34487 patients(0.08%) who received prophylactic antibiotics and 16 cases in 10911(0.15%) who received none [risk ratio(RR) = 0.53,95% confidence interval(CI): 0.29-0.99,P = 0.05]. A sub-group analysis in which bony procedures were excluded was performed which found no significant difference in infection rates between patients that received prophylactic antibiotics and patients that did not(P > 0.05). All anterior cruciate ligament reconstruction studies used prophylactic antibiotics,but two studies investigating the effect of soaking the graft in vancomycin in addition to standard intravenous(IV) prophylaxis were combined for analysis. There were 19 cases in 1095 patients(1.74%) who received IV antibioitics alone and no infections in 2034 patients who received IV antibiotics and had a vancomycin soaked graft(RR = 0.01,95%CI: 0.001-0.229,P < 0.01).CONCLUSION Prophylactic antibiotics are effective in preventing septic arthritis following simple knee arthroscopy. In procedures involving graft implantation,graft soaking reduces the rate of infection. | John Carney Nathanael Heckmann Erik N Mayer Ram K Alluri Carleton Thomas Vangsness George F Hatch Ⅲ Alexander E Weber | 2018 | World Journal of Orthopedics2018，9，11： | 2 |
| 3 | Reoperation for prosthetic aortic valve obstruction in the era of echocardiography: trends in diagnostic testing and comparison with surgical findings显示文摘 | Steven E Girard Fletcher A Miller Thomas A Orszulak Charles J Mullany Samantha Montgomery William D Edwards Henry D Tazelaar Joseph F Malouf A.Jamil Tajik | 2001 | Journal of the American College of Cardiology2001，，2： | 2 |
| 4 | Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells显示文摘AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts 'antisense-toxin-RNA' into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection. | Peter Hafkemeyer Ulrich Brinkmann Elizabeth Brinkmann Ira Pastan Hubert E Blum Thomas F Baumert | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 5 | Robust analysis of the yeast proteome under 50 kDa by molecular-massbased fractionation and top-down mass spectrometry显示文摘 | Kellie J F Catherman A D Durbin K R Tran J C Tipton J D Norris J L Witkowski C E 2nd Thomas P M Kelleher N L | | 0，，01： | 1 |
| 6 | Transient electromag- netic interference in substations显示文摘 | Wiggins C M Thomas D E Nickel F S | 1994 | IEEE Transations on Power Delivery1994，9，4： | 1 |
| 7 | effects of solvent steric properties on the equilibrium swelling and kinetics of solvent swelling of coal显示文摘 | Ndaji F E Thomas K M | 1995 | Fuel1995，74，6： | 1 |
| 8 | Transient electromagnetic interference in substations显示文摘 | Wiggins C M Thomas D E Nickel F S | 1994 | IEEE Trans on PWRD1994，9，4： | 1 |
| 9 | Fuel options for the fuel cell vehicle:hydrogen,methanol or gasoline显示文摘 | Thomas C E James B D Lomax F D | 2000 | Hydrogen Energy2000，25，： | 1 |
| 10 | Effect of solvent basicity on the kinetics of solvent swelling of coal显示文摘 | NDAJI F E THOMAS K M | 1993 | Fuel1993，72，11： | 1 |
| 11 | Platform-based product design and environmental turbulence显示文摘 | Thomas E F | 2014 | European Journal of Innovation Management2014，17，1： | 1 |
| 12 | Mechanical be-havior of acrylonitrile butadiene styrene fused deposition materials modeling显示文摘 | Rodriguez J F Thomas J P Renaud J E | 2003 | Rapid Prototyping Journal2003，9，4： | 1 |
| 13 | Transient electromag- netic interference in substations显示文摘 | Wiggins C M Thomas D E Nickel F S | 1994 | IEEE Transactions on Power Delivery1994，9，4： | 1 |
| 14 | Immunogenicity, safety, and tolerability of a recombinant mcaslcs-virus-based chikungunya vaccine: a randomised, double-blind, placebo-controlled, active-comparator, first- in-man trial 显示文摘 | Ramsauer K Schwameis M Firbas C Milliner M Putnak ILl Thomas SJ Despr6s P Tauber E Jilma B Tangy F | 2015 | Lancet Infect Dis2015，15，5： | 1 |
| 15 | Estimating the use of veterinary medicines in the European union显示文摘 | Stefan A E K Johann F M Thomas K | 2008 | Regulatory Toxicology ald Phar- macology2008，50，1： | 1 |
| 16 | Lung injury from gut ischemia:Insensitivity to portal blood flow diversion显示文摘 | JOHNSON D E RONALD F | 1994 | J Trauma1994，35，4： | 1 |
| 17 | Arthropod abundance and diversity in differently vegetated margins of arable fields显示文摘 | Thomas C F G Marshall E J P | 1999 | Agriculture Ecosystem and Environment1999，72，： | 1 |
| 18 | Isolation of Angiopoietin-1, a Ligand for the TIE2 Receptor, by Secretion-Trap Expression Cloning显示文摘 | Samuel Davis Thomas H Aldrich Pamela F Jones Ann Acheson Debra L Compton Vivek Jain Terence E Ryan Joanne Bruno Czeslaw Radziejewski Peter C Maisonpierre George D Yancopoulos | 1996 | Cell1996，，7： | 1 |
| 19 | Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia显示文摘 | F Xie Y Hu SE Turvey LA Magee RM Brunham K‐C Choi M Krajden PCK Leung DM Money DM Patrick E Thomas P von Dadelszen | 2009 | BJOG: An International Journal of Obstetrics & Gy2009，，： | 1 |
| 20 | Impotence in a patient with rheumatoid arthritis treated with methotrexate (letter)显示文摘 | Thomas E Koumouvi K Blotman F | 2000 | J Rheumatol2000，27，7： | 1 |
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