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| 1 | Nitrite-derived nitric oxide by xanthine oxidoreductase protects the liver against ischemia- reperfusion injury显示文摘It was demonstrated that xanthine oxidoreductase (XOR), during ischemia, catalyzes the formation of nitric oxide (NO) from nitrite (NO2-) and this NO2--derived NO protects the isolated perfused rat heart against the damaging effects of ischemia-reperfusion (I/R) when conventional nitric oxide synthase (NOS) -dependent NO production is impaired. Liver is one of the organs with the highest XOR concentration. This study was designed to determine whether NO2--derived NO by XOR protects liver against I/R injury in vivo. For its minute amounts and active reactivity, NO can not be detected directly in real time in vivo by this time. We have to prove the above hypothesis indirectly. METHODS:Wistar rats were pretreated with saline, NOS inhibitor L-NAME (10 mg/kg intravenously), XOR inhibitor allopurinol (1.5 mg/kg orally), L-NAME +allopurinol and NO scavenger carboxy-PTIO (0.6 mg/kg intravenously) respectively (12 animals per group). And then, they were subjected to total liver ischemia for 40 minutes followed by reperfusion. Blood samples and liver tissues were obtained for analysis after 3 hours of reperfusion. Survival was also investigated. RESULTS:Allopurinol-treated animals exhibited further increased serum alanine aminotransferase (ALT) levels and liver myeloperoxidase (MPO) activities, but further decreased liver adenosine triphosphate (ATP) stores after I/R compared to saline-treated counterparts (830.5±108.3 U/L, 56.5±11.0 U/mg protein and 1.93±0.47 μmol/g vs. 505.8± 184.2 U/L, 41.5±10.2 U/mg protein and 3.05±0.55 μmol/g respectively, P<0.01, P<0.05 and P<0.01 respectively). The hepatocyte injury was further exacerbated and the overall survival rate was significantly decreased after I/R in animals given by allopurinol compared to those pretreated by saline (P<0.05). L-NAME and allopurinol co-treated animals exhibited more severe liver injury (P<0.05 and P<0.01) and a further decreased overall survival rate (P<0.05) compared to L-NAME or allopurinol alone-treated counterparts, but they were not different from carboxy-PTIO treated animals (P>0.05). CONCLUSION:NO2--derived NO by XOR in the hypoxic and acidic environment induced by hepatic I/R protects the liver against I/R injury in vivo. | Department of General Surgery ( Lu P, Wang CY and Chen DD), and Department of Radiology (Liu F), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Cold Spring Harbor Laboratory, New York 11724, USA ( Yao Z) General Surgery Laboratory, Union Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430022 , China (Tian Y, Zhang JH and Wu YH) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 4 |
| 2 | 饲料中添加蛋白酶对肉鸡性能、养分利用率、肠道形态和胴体品质的影响显示文摘试验在饲喂玉米、高粱为主的肉鸡日粮中补充大豆,并添加蛋白酶,研究蛋白酶对肉鸡性能、养分消化率、肠道形态和胴体品质的影响。试验选取1日龄AA肉仔鸡(43.8±1.31 g)256只,均为公鸡,随机分配到2×2试验设计方阵中,方阵包括正常组(玉米组和高粱组)和蛋白酶组(空白组和添加蛋白酶150 mg·kg^(-1)的试验组)。试验1~21 d为第一阶段,22~42 d为第二阶段。试验结果表明,第二阶段中,日粮中添加蛋白酶极显著提高肉鸡的平均日增重(ADG,P<0.01),在整个试验过程中显著提高(P<0.05)。在各个时期,正常组对肉鸡生产性能均无显著影响(P>0.05)。19~21 d时,添加蛋白酶可显著提高肉鸡的表观代谢能(AME),干物质(DM)、总能量(GE)和养分消化率(P<0.05)。此外,日粮以高粱为主时,AME、GE消化率有降低趋势(P=0.05),氮沉积率也有降低趋势(P=0.08)。40~42 d时,日粮中添加蛋白酶可显著提高AME,DM、GE和氮沉积率(P<0.05)。21 d时,日粮中添加蛋白酶极显著增加肠绒毛高度、肠绒毛与隐窝深度比值,极显著降低十二指肠、空肠、回肠的隐窝深度(P<0.01)。42 d时,日粮中添加蛋白酶显著增加空肠肠绒毛高度、绒毛高度与隐窝深度比值,显著降低隐窝深度(P<0.05)。日粮中添加蛋白酶显著增加胸肌重、24 h p H,显著降低胸肌的滴水损失(P<0.05)。试验结果表明,饲养阶段日粮中添加复合蛋白酶可提高肉鸡ADG;在第一阶段可改善AME、DM、GE和氮沉积率和肠道形态。补充蛋白酶可提高出栏重、胸肌重和24 h pH,降低胸肌的滴水损失。低单宁高粱日粮与玉米日粮相比,对肉鸡性能的影响相近。 | Xu X Wang H L Pan L Ma X K Tian Q Y Xu Y T Long S F Zhang Z H Piao X S | 2017 | 饲料博览2017，0，1： | 2 |
| 3 | Sequence concerning hamiltonicity of graphs 显示文摘 | LIU Y TIAN F WU Z | 1995 | Nanjing Normal University (Natural Science)1995，18，1： | 2 |
| 4 | Numerical modelling and validation of gas-particle flow in an in-line tube bank显示文摘 | Tian Z F Tu J Y Yeoh G H | 2007 | Computers & Chemical Engineering2007，31，9： | 1 |
| 5 | Straight free-edge effects in laminated composites显示文摘 | Tian Z Zhao F Yang Q | 2004 | Finite Elements in Analysis and Design2004，41，1： | 1 |
| 6 | Microwave-assisted one pot synthesis of water-soluble rare earth doped fluoride luminescent nanoparti cles with tunahle colors显示文摘 | Mi C C Tian Z H Han B F | 2012 | J Alloy Compd2012，525，15： | 1 |
| 7 | HSO4 acidic ionic liquid as a novel, efficient, and environmentally benign catalyst for synthesis of 1, 5-benzodiazepines under mild conditions显示文摘 | Du Y Y Tian F L Zhao W Z | 2006 | Synthetic Communications2006，36，12： | 1 |
| 8 | Influenced of yttrium on microstructure,mechanical properties and deformability of Ti-43Al-9V alloy显示文摘 | Chen Y Y Kong F T Han J C Chen Z Y Tian J | 2005 | Intermetallics2005，13，34： | 1 |
| 9 | Highly virulent porcine reproductive and respiratory syndrome virus emerged in China显示文摘 | Zhou Y J Hao X F Tian Z J | 2008 | Transbound Emerg Dis2008，55，34： | 1 |
| 10 | Comparison of mierostructures and mechanical properties of a Cu-Ag alloy processed using different severe plastic deformation modes显示文摘 | Tian Y Z Wu S D Zhang Z F | 2011 | Materials Science and Engineering2011，528，： | 1 |
| 11 | Preparation and mechanical properties of cotton stalk bast fibers reinforced polylactic acid biodegradable composites显示文摘 | WU Z WEI C Y TIAN Z F | | 0，，： | 1 |
| 12 | Higyly virulent porcine reproductive and respiratory syndrome virus emerged in China显示文摘 | Zhou Y J Hao X F Tian Z J | | 0，，： | 1 |
| 13 | Enhanced adsorption desulfurization performance over hierarchically structured zeolite Y显示文摘 | TIAN F P SHEN Q C FU Z K WU Y H JIA C Y | 2014 | Fuel Process Technol2014，128，： | 1 |
| 14 | Prognostic significance of tumor grading and staging in mammary carcinomas with neuroendocrine differentiation 显示文摘 | Tian Z Wei B Tang F | 2011 | Hum Pathol2011，42，8： | 1 |
| 15 | Optimized of AM and AA Copolymerizing Reaction Condition by Thermal Inducing显示文摘 | Yan X L Yan W L Tian Z F | 2009 | JournaL of YiLi NormaL University (Natural Science Edition)2009，，2： | 1 |
| 16 | Effects of small amount addition of rare earth Er on microstructure and property of SnAgCu solder 显示文摘 | Shi Y W Tian J Hao H Xia Z D Lei Y P Guo F | 2008 | Journal of Alloys and Compounds2008，453，12： | 1 |
| 17 | Hydrogen-rich saline re- duces the oxidative stress and relieves the severity of trau- ma-induced acute pancreatitis in rats显示文摘 | Ren J Luo Z Tian F | 2012 | Journal of Trau- ma and Acute Care Surgery2012，72，6： | 1 |
| 18 | Cytotoxieity of three cycloartane triterpenoids from Cimicifuga dahurica 显示文摘 | Tian Z Yang M Huang F | 2005 | Cancer Lett2005，226，1： | 1 |
| 19 | Preparation of high loading Pt supported on carbon by on site reduction显示文摘 | Tian Z Q Xie F Y Shen P K | 2004 | J Mater Sci2004，39，4： | 1 |
| 20 | Hot Deformation and Processing Map of 15% SiCp/2009 Al Composite 显示文摘 | Xiao B L Fan J Z Tian X F | 2005 | Journal of Materials Science2005，40，： | 1 |
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