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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Erosion Function Apparatus for Scour Rate Predictions显示文摘 | Briaud J L Ting F C K | 2001 | Journal of Geoteehnical and geoenvironmental engi- neering2001，127，2： | 1 |
| 3 | A model to predict the development of mental status changes of unclear causeafler liver transplantation显示文摘 | Kanwal F Chen D Ting L | 2003 | Liver Transpl2003，9，12： | 1 |
| 4 | Vitamin C improves endothelium-depentent vasodilation in patients with non-insulin-dependent diatetes mellitus显示文摘 | Ting H H Timimi F K Boles K S | 1996 | Chin invest1996，97，： | 1 |
| 5 | Comparative studies of the reflectivity of vitrinite and sporinite显示文摘 | Ting F T C Sitler J A | | 0，，3： | 1 |
| 6 | Uptake of cadmium and zinc by the alga Chlorella vulgaris (II):Multi-ion situation显示文摘 | TING Y P PRINCE I G LAWSON F | 1991 | Biotechnol Bioeng1991，37，： | 1 |
| 7 | The addition of hydrogen to a gasoline-fuelled SI engine显示文摘 | HENSHAW P F TING D S K | 2004 | International Journal of Hydrogen Energy2004，，29： | 1 |
| 8 | Northern winter stationary waves:Theory and modeling显示文摘 | Held I M Ting M F Wang H L | | 0，，： | 1 |
| 9 | Structural response ofthermally restrained steel columns under axial loads 显示文摘 | Huang Z F Tsn K H Ting S K | 2000 | Journal of Structural Engineering ASCE2000，90，4： | 1 |
| 10 | Design of fuzzy controller for active suspension system显示文摘 | Ting C S Kung F C | 1995 | Control System Laboratory1995，5，4： | 1 |
| 11 | The addition of hy- drogen to a gasoline-fueled SI engine 显示文摘 | Andrea T D Henshaw P F Ting D S K | 2004 | International Journal of Hydrogen Energy2004，，29： | 1 |
| 12 | Accurate measure- ment of stable isotopes of lithium by inductively coupled plasma mass spectrometry 显示文摘 | Sun X F Ting B T G Zeisel S H | 1987 | Analyst1987，，112： | 1 |
| 13 | Erosion function apparatus for scour rate predictions显示文摘 | BRIAUD J L TING F C K CHEN H C | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，127，2： | 1 |
| 14 | 显示文摘 | Jianxin M Ming F Ngai Ting L | 1996 | J Catal1996，158，： | 1 |
| 15 | An improved meanshift insulator image segmentation algorithm 显示文摘 | TING F ZHAO Y B HU X L | 2013 | Advanced Materials Research2013，，： | 1 |
| 16 | Calcium phosphate nanoparticle as a novel nonviral vector efficiently transfection of DNA in cancer gene therapy显示文摘 | TING L TANG A F CHEN Y X et ol | 2005 | Cancer Biother Radiopharm2005，20，2： | 1 |
| 17 | Observation of undertow and turbulence in a laboratory surf zone 显示文摘 | TING F C K KIRBY J T | 1994 | Coastal Engineering1994，24，： | 1 |
| 18 | The gut microbiota as an environmental factor that regulates fat storage 显示文摘 | Backhed F Ding Hao Wang Ting | 2004 | PANS2004，101，15： | 1 |
| 19 | Comparative analysis of SmartArc -based dual arc volumetric- modulated are radiotherapy (VMAT) versus intensity- modulated radiotherapy (IMRT) for nasopharyngeal carcinoma显示文摘 | LEE T F CHAO P J TING H M | 2011 | J Appl Clin Med Phys2011，12，4： | 1 |
| 20 | Erosion function apparatus for scour rate predictions 显示文摘 | BRIAUD J L TING F C K CHEN H C | 2001 | Journal of Geotechnical and Geoenvironmental Engineering2001，127，2： | 1 |
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