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| 1 | The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice显示文摘Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases(PKSs) are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase(OsPKS2) in male reproduction of rice(Oryza sativa). Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT(wild type), ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds.However, unlike pksb/lap5, in which most detected phenolics were substantially decreased, ospks2 accumulated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/lap5, we propose that PKS proteins have functionally diversified during evolution.Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants. | Xiaolei Zhu Jing Yu Jianxin Shi Takayuki Tohge Alisdair R.Fernie Sagit Meir Asaph Aharoni Dawei Xu Dabing Zhang Wanqi Liang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 9 |
| 2 | The Metabolic Response of Arabidopsis Roots to Oxidative Stress is Distinct from that of Heterotrophic Cells in Culture and Highlights a Complex Relationship between the Levels of Transcripts, Metabolites, and Flux显示文摘新陈代谢的调整是一重要,但是糟糕理解,到氧化应力的植物的反应的部分。在以前的研究(巴斯斯特等, 2007 ) ,在到由甲蔡醌的氧化应力的正式就职的文化的 Arabidopsis 房间的新陈代谢的反应被描绘。紧急情况幸存策略在 anabolism 的主要新陈代谢大部分在哪个是下面调整的赞成被揭开分解代谢并且抗氧化剂新陈代谢。在整个植物纸巾的反应可能是不同的,我们因此调查了 Arabidopsis 根的反应到甲蔡醌处理,分析有主要以及第二等的新陈代谢的焦点的 transcriptome, metabolome 和关键新陈代谢的流动。用氧化还原作用敏感的 GFP,甲蔡醌引起氧化还原作用不安,这也被显示出,不是就在线粒体,而且在根的 cytosol 和质体。在里面开始的 30 ? 处理的 min,反应类似于房间文化:在 TCA 周期和氨基酸生合成的代谢物有减少, transcriptomic 反应被 DNA 规章的蛋白质的起来规定统治。在 2 和 6 以后 ? 处理的 h,根的反应对房间文化不同。代谢物层次没仍然保持沮丧,但是相反恢复并且在 pyruvate 的情况中,一些氨基酸和脂肪族的 glucosinolates 在控制层次上面显示出稳定的增加。然而,在中央碳新陈代谢的流动的主要变化都没被观察,新陈代谢的抄本主要独立于相应代谢物变化了。一起,结果建议根纸巾能在氧化抑制以后恢复新陈代谢的活动并且为 glycolysis 和氧化戊糖磷酸盐小径加亮潜在地重要的角色。 | Martin Lehmann Markus Schwarzlander Toshihiro Obata Supaart Sirikantaramas Meike Burow Carl Erik Olsen Takayuki Tohge Mark D. Fricker Birger Lindberg Moiler Alisdair R. Fernie Lee J. Sweetlove Miriam Laxa | 2009 | Molecular Plant2009，2，3： | 8 |
| 3 | Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade显示文摘Steroidal glycoalkaloids(SGAs) are nitrogen-containing secondary metabolites of the Solanum species,which are known to have large chemical and bioactive diversity in nature.While recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato,the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical formula.Here we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA biosynthesis.In order to elucidate natural diversity of SGA biosynthesis,a total of 169 putative SGAs found in eight tomato accessions(Soianum lycopersicum,S.pimpinellifolium,S.cheesmaniae,S.chmielewskii,S.neorickii,S.peruvianum,S.habrochaites,S.pennellii) and four tissue types were used for correlation analysis.The results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch points.As such this represents a novel strategy for peak annotation for plant secondary metabolites. | Kevin Schwahn Leonardo Perez de Souza Alisdair R.Fernie Takayuki Tohge | 2014 | Journal of Integrative Plant Biology2014，56，9： | 6 |
| 4 | Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex显示文摘While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across species.In this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of tomato.For this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic genes.The combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid pathway.The combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit ripening.Our results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways. | Takayuki Tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie | 2020 | Molecular Plant2020，13，7： | 3 |
| 5 | 一个稻属特异存在的芳香族酚胺基因簇助力水稻广谱抗性显示文摘代谢基因簇(Biosynthetic Gene Cluster)由多个来源于不同酶家族的基因在染色体上相邻或是相近的位置组成.近十年来的研究发现,许多参与植物防御和具有重要医药价值的植物次生代谢物由植物代谢基因簇编码合成,但对此类基因簇的形成过程、生理功能以及进化等方面知之甚少.本研究综合利用基于代谢物的全基因组关联分析(m GWAS)、体外酶活力测定、植物稳定转化和烟草瞬时表达等多种手段在水稻10号染色体上发现并鉴定了一个芳香族酚胺基因簇——羟基肉桂酰酪胺(HT)基因簇.该基因簇由1个吡哆醇5′-磷酸氧化酶、1个酪氨酸脱羧酶和2个酰基转移酶组成,其终产物羟基肉桂酰酪胺具有植物抗毒素效应,能够显著提高水稻对两大典型病害(白叶枯和稻瘟病)的抗性.比较基因组学证据显示转座子(TEs)在HT基因簇关键组分的组装中发挥重要作用,侧面印证该基因簇的形成兼具'解毒'和'抗病'双重功效.进一步的进化分析表明, HT基因簇在稻属AA基因组分支中特异存在,其关键组分在这些植物中存在基因拷贝数和相对位置的变异.综上,本研究揭示了水稻芳香族酚胺基因簇的生化基础及其在水稻广谱抗性方面的作用,不仅为作物抗性方面的遗传改良提供了新资源,也为植物中代谢基因簇的形成机制和进化模式等方面提供了新的见解. | 沈双欠 彭梦 方红 王梓烜 周申 靖鑫宇 张猛 杨陈坤 郭昊 李宇飞 雷龙 施宇恒 孙阳阳 刘贤青 许从萍 Takayuki Tohge 袁猛 Alisdair R.Fernie 宁约瑟 王国梁 罗杰 | 2021 | Science Bulletin2021，66，23： | 3 |
| 6 | Mapping theArabidopsis Metabolic Landscape by Untargeted Metabolomics at Different Environmental Conditions显示文摘新陈代谢的染色体宽的协会研究(mGWAS ) ,因此,代谢物层次被认为是特点,帮助能解开新陈代谢的网络的基因基础。309 Arabidopsis 就职的一个总数在二个独立环境条件(控制和应力) 下面被种并且使遭到到介绍的 untargeted LC-MS-based metabolomic;获得的吸水的代谢物的层次在 GWAS 被使用。我们为超过 3000 半极的代谢物的 two-condition-based GWAS 导致了 123 高度解决的代谢物的察觉量的特点 loci ?(p 1.0E-08 ) 。有趣地,不同地,从在 Arabidopsis 主要代谢物,趋于被很多小效果的 loci 控制,的自然变化,我们在发现几个学生大效果的 loci 控制第二等的代谢物的变化的小效果的 loci 的一个广阔数字。two-condition-based GWAS 被集成用一个时间功课压力实验与导出网络的代谢物抄本关联跟随。通过这条综合途径,我们选择了在结构的基因和代谢物之间的 70 个关键候选人协会,并且试验性地验证了八个新奇协会,他们中的二个在二环境的出现微分基因规定学习了。我们表明联合有 time-course-derived 网络的基于 metabolomics 的 GWAS 两个都为识别代谢物基因协会在不同不能生活的环境下面执行了的大规模 untargeted 的力量,提供进 Arabidopsis 的新陈代谢的风景的新奇全球卓见。 | Si Wu Takayuki Tohge Alvaro Cuadros-lnostroza Hao Tong Hezi Tenenboim Rik Kooke Michael Meret Joost B. Keurentjes Zoran Nikoloski Alisdair R. Fernie Lothar Willmitzer Yariv Brotman | 2018 | Molecular Plant2018，11，1： | 3 |
| 7 | Analysis of the Interface between Primary and Secondary Metabolism in Catharanthus roseus Cell Cultures Using ^13C-Stable Isotope Feeding and Coupled Mass Spectrometry显示文摘Dear Editor, The tropical plant Madagascar periwinkle Catharanthus roseus (L.) G.Don is a rich source of plant-derived medicinal terpenoid indole alkaloids (TIAs),including the anti-hypertensive ajmalicine,the sedative compound serpentine,and the anticancer drugs vinblastine and vincristine.However,the latter two compounds are produced in C roseus plants only in very low amounts.Elicitors such as hormones (e.g.jasmonates or salicylic acid) activate plant natural defense responses,including increased secondary metabolite production (EI-Sayed and Verpoorte,2007;Lackman et al.,2011).There are two different metabolic pathways for the biosynthesis of terpenoids leading to the formation of the Cs central precursor isopentenyl diphosphate (IPP),namely the classical cytosolic mevalonate pathway and the mevalonate-independent plastidic 2-C-methyI-D-erythritol 4-phosphate (MEP) pathway. | Carla Antonio Natali R. Mustafa Sonia Osorio Takayuki Tohge Patrick Giavalbco Lothar Willmitzer Heiko Rischer Kirsi-Marj Oksman-Caldentey Robert Verpoorte Alisdair R. Fernie | 2013 | Molecular Plant2013，6，2： | 2 |
| 8 | Effects of A1 and Ca in ferrosilicon alloys for deoxidation on inclusion composition in type 304 stainless steel 显示文摘 | Mizuno K Todoroki H Noda M Tohge T | 2001 | Iron Steelmaker2001，，8： | 2 |
| 9 | Analysis of Short-Term Metabolic Alterations in Arabidopsis Following Changes in the Prevailing Environmental Conditions显示文摘尽管我们有关到相反的 growthconditions 的新陈代谢的调整的重要性的知识的可观的增加最近被提供了,相对,很少对响应 fluctuationin 发生的调整被知道环境因素。评估发生在这样改变环境条件下面的新陈代谢的调整增加我们在是影响 bysuch 的主要小径之间的串音的当前的理解的好机会调节的 offersa。到这个目的,在正常条件下面成长的植物被转移到不同的光,温度 conditionswhich 被期望影响(在另外的过程之中) 光合作用和 photorespiration 和 characterizedat 的率跟随这转变的生理、分子、新陈代谢的层次。我们的结果揭示了类似的行为 inresponse 到处理并且与植物 metabolism.They 的小径进一步加亮的专业一起暗示 photorespiration 的一个紧密的连接这些小径的规定的多数没在叶新陈代谢是的 transcriptionbut 的水平被调停,这相当预先平衡在这些输入 parameters.Key 适应变化词: | Alexandra Florian Zoran Nikoloski Ronan Sulpice Stefan Timm Wagner L. Araujo Takayuki Tohge Hermann Bauwe Alisdair R. Fernie | 2014 | Molecular Plant2014，7，5： | 2 |
| 10 | Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures显示文摘As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the atmosphere.Given the indiscriminant nature of Rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas composition.However,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in general.Moreover,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains unknown.To address these questions,plants Arabidopsis thaliana(Arabidopsis) ecotype Columbia(Col-O) grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this transition.The results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite levels.The results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription. | Alexandra Florian Stefan Timm Zoran Nikoloski Takayuki Tohge Hermann Bauwe Wagner L.Araujo Alisdair R.Fernie | 2014 | Journal of Integrative Plant Biology2014，56，9： | 2 |
| 11 | Direct preparation of fine powders of the 80 k superconducting phase in the Bi-Pb-Ca-Sr-Cu-O system by spray-pyrolysis显示文摘 | Tohge N Tatsumisago M Minamie T | 1989 | Japanese Journal Apllied Physics1989，28，7： | 1 |
| 12 | Preparation of LiTi_2(PO_4)_3 by the Sol-gel Method and Investigation of Its Formation Process显示文摘LiTi2(PO4)3ceramicsmayacquireahighelectricalconductivitybypartialysubstitutingsomeM3+ions(M=Al,Sc,Y,La)fortheTi4+ionsordoping... | ZHU Jishun**, Tohge Noboruand Minami Tsutomu (Department of Chemistry, Shanghai Normal University, Shanghai, 200234 Department of Applied Chemistry, University of Osaka Prefecture, 4804, Mozuumemachi, Sakaishi, Osaka 591, Japan) | 1999 | Chemical Research in Chinese Universities1999，15，2： | 1 |
| 13 | Members of the LBD family of transcription factors repress anthocyanin synthesis and affect additional nitrogen responses in Arabidopsis显示文摘 | RUBIN G TOHGE T MATSUDA F | 2009 | The Plant Cell2009，21，11： | 1 |
| 14 | Thoracic myelopathy caused by ossification of the ligamentum flavum Clinicopathologic study and surgical treatment 显示文摘 | Okada K Oka S Tohge K | 1991 | Spine (Phila Pa 1976)1991，16，3： | 1 |
| 15 | Thoracic myelopathy caused by ossification of the ligementum flavum:Clinicopathologic study and surgical treatment显示文摘 | Oka S Tohge K | 1991 | Spine1991，16，： | 1 |
| 16 | Members of the LBD family of transcription factors repress anthocyanin synthesis and affect additional nitro- gen responses in Arabidopsis显示文摘 | Rubin G Tohge T Matsuda F | 2009 | The Plant Cell2009，21，： | 1 |
| 17 | Preparation of Pho- tosensitive Gel Films And Fine Patterning of A- morphous A12 03-SIO2 Thin Films 显示文摘 | Gaoyang Zhao Noboru Tohge | 1998 | Materials research Bulletin1998，33，1： | 1 |
| 18 | Thoracic myelopa thy caused by ossification of the ligament flavum clinicopathologic study and surgical treatment 显示文摘 | Okada K Oka S Tohge K | 1991 | Spine1991，16，3： | 1 |
| 19 | Members of the LBD family of transcription factors repress anthocyanin synthesis and affect additional nitrogen responses in Arabidopsis显示文摘 | Rubin G Tohge T Matsuda F | 2009 | The Plant Cell2009，21，： | 1 |
| 20 | Thoracic myelopathy caused by ossi-fication of the ligamentum flavum:clinicopathologic study and surgi-cal treatment显示文摘 | Okada K Oka S Tohge K | 1991 | Spine(Phila Pa 1976)1991，16，3： | 1 |
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