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1篇 您的检索式:作者名="TONGGU LiGe"
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1Membrane protein reconstitution for functional and structural studies显示文摘Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.WANG LiGuo TONGGU LiGe 2015Science China(Life Sciences)2015,58,1:5
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