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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Effect of coronary angioplasty on precordial QT dispersion 显示文摘 | Yunus A Gillis AM Traboulsi M | 1999 | Am J Cardiol1999，79，10： | 1 |
| 3 | Rescue angioplasty during myocardial infarction has a beneficial effect on mortality: A tenable hypothesis显示文摘 | Belenkie I Traboulsi M Hall CA | 1992 | Can J Cardiol1992，8，4： | 1 |
| 4 | Lymphedema-distichiasis syndrome and FOXC2 gene mutation显示文摘 | Traboulsi EI Al-Khayer K Matsumoto M | 2002 | Am J Ophthalmol2002，134，4： | 1 |
| 5 | Effect of coronary angioplasty on precordial QT dispersion显示文摘 | Yunus A Gillis AM Traboulsi M | 1997 | Am J Cardiol1997，79，： | 1 |
| 6 | Effect of coronary angioplasty on precordial QT dispersion显示文摘 | Yunus A Gillis A M Traboulsi M | 1997 | Am J Cardiol1997，79，10： | 1 |
| 7 | Dominant radial drusen and Arg345Trp EFEMP1 mutation显示文摘 | Matsumoto M Traboulsi EI | 2001 | Am J Ophthalmol2001，131，6： | 1 |
| 8 | Diagnosis and management of acute ischemic stroke:speed is critical显示文摘 | Musuka TD Wilton SB Traboulsi M | 2015 | CMAJ2015，187，12： | 1 |
| 9 | Repellency and Toxicity of aromatic Plant extracts against the mosquito Culex Pipiens molestus(Diptera:Culicidae)显示文摘 | Traboulsi AF EL-Haj S Tueni M | 2005 | Pest Manage Sci2005，61，： | 1 |
| 10 | Synthesis, characterization and photophysical properties of benzidine-based compounds显示文摘 | HMADEH M TRABOULSI H ELHABIRI M | 2008 | Tetrahedron2008，64，27： | 1 |
| 11 | Malignant melanoma of the conjunctiva in children: a review of the international literature 1965-2006 显示文摘 | Taban M Traboulsi EI | 2007 | J Pediatr Ophthalmol Strabismus2007，44，5： | 1 |
| 12 | Effect of coronary angioplasty on precordial QT dispersion显示文摘 | Yunus A Gilis AM Traboulsi M | 1997 | Am J Cardiol1997，79，： | 1 |
| 13 | Effect of coronary angioplasty on precordial QT dispersion 显示文摘 | Yunus A Gillis AM Traboulsi M | 1997 | Am J cardiol1997，79，10： | 1 |
| 14 | Effect of coronary angioplasty on cardial QT dispersion显示文摘 | Yunus A Gillis AM Traboulsi M | 1997 | Am J Cardiol1997，79，10： | 1 |
| 15 | Antiarrhythmic drug effects on QT interval dispersion in patientsundergoing electrophar-macologic testing for ventrieular tachycardia and fibrillation显示文摘 | Gillis AM Traboulsi M Hii TY | 1998 | Am J Cardiol1998，81，5： | 1 |
| 16 | Antiarrhythmic drug effects on interval dispersion in patients undergoing electropharmacologic testing for ventricular tachycardia and fibeillation显示文摘 | GILLIS A M TRABOULSI M HII J T Y | 1998 | Am J Cardiol1998，81，： | 1 |
| 17 | Repellency and toxicity of aromatic plant extracts against the mosquito Culex pipiens molestus (Diptera: Culicidae)显示文摘 | Traboulsi A F El -Haj S Tueni M | 2005 | Pest Management Science2005，61，： | 1 |
| 18 | Effect of coronary angioplasty on precordial QT dispersion显示文摘 | Yunus A Gillis A M Traboulsi M | 1997 | Am J Cardiol1997，79，10： | 1 |
| 19 | Rescue angioplasty dur- ing myocardial infarction has a beneficial effect on mortality, a tenable hypothesis显示文摘 | Belenkie I Traboulsi M Hall CA | 1992 | Can J Cardioh1992，8，： | 1 |
| 20 | Dispersion of ventricular reploarization predicts antiarrhythmic drug response显示文摘 | Traboulsi M Wyse DG | 1993 | Circulation1993，88，： | 1 |
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