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| 1 | Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy显示文摘Background:Parkinson’s disease(PD)is a neurodegenerative disorder whose diagnosis is often challenging because symptoms may overlap with neurodegenerative parkinsonisms.PD is characterized by intraneuronal accumulation of abnormalα-synuclein in brainstem while neurodegenerative parkinsonisms might be associated with accumulation of eitherα-synuclein,as in the case of Multiple System Atrophy(MSA)or tau,as in the case of Corticobasal Degeneration(CBD)and Progressive Supranuclear Palsy(PSP),in other disease-specific brain regions.Definite diagnosis of all these diseases can be formulated only neuropathologically by detection and localization ofα-synuclein or tau aggregates in the brain.Compelling evidence suggests that trace-amount of these proteins can appear in peripheral tissues,including receptor neurons of the olfactory mucosa(OM).Methods:We have set and standardized the experimental conditions to extend the ultrasensitive Real Time Quaking Induced Conversion(RT-QuIC)assay for OM analysis.In particular,by using human recombinantα-synuclein as substrate of reaction,we have assessed the ability of OM collected from patients with clinical diagnoses of PD and MSA to induceα-synuclein aggregation,and compared their seeding ability to that of OM samples collected from patients with clinical diagnoses of CBD and PSP.Results:Our results showed that a significant percentage of MSA and PD samples inducedα-synuclein aggregation with high efficiency,but also few samples of patients with the clinical diagnosis of CBD and PSP caused the same effect.Notably,the final RT-QuIC aggregates obtained from MSA and PD samples owned peculiar biochemical and morphological features potentially enabling their discrimination.Conclusions:Our study provide the proof-of-concept that olfactory mucosa samples collected from patients with PD and MSA possess important seeding activities forα-synuclein.Additional studies are required for(i)estimating sensitivity and specificity of the technique and for(ii)evaluating its application for the diagnosis of PD and neurodegenerative parkinsonisms.RT-QuIC analyses of OM and cerebrospinal fluid(CSF)can be combined with the aim of increasing the overall diagnostic accuracy of these diseases,especially in the early stages. | Chiara Maria Giulia De Luca Antonio Emanuele Elia Sara Maria Portaleone Federico Angelo Cazzaniga Martina Rossi Edoardo Bistaffa Elena De Cecco Joanna Narkiewicz Giulia Salzano Olga Carletta Luigi Romito Grazia Devigili Paola Soliveri Pietro Tiraboschi Giuseppe Legname Fabrizio Tagliavini Roberto Eleopra Giorgio Giaccone Fabio Moda | 2019 | Translational Neurodegeneration2019，8，1： | 5 |
| 2 | Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization显示文摘 | Fontana F Tagliavini J Congiu L | 1998 | Marine Biology1998，132，3： | 1 |
| 3 | Iron deficiency and chlorosis in orchard and vineyard ecosystems显示文摘 | TAGLIAVINI M ROMBOLA A D | 2001 | European Journal of Agronomy2001，15，2： | 1 |
| 4 | Pear(Pyrus communis) and quince (Cydonia oblonga) roots exhibitdifferent ability to prevent sodium and chloride uptake whenirrigated with saline water显示文摘 | Stefano Musacchi Maurizio Quartieri Massimo Tagliavini | 2006 | European Journal of Agronomy2006，，24： | 1 |
| 5 | Important source of marine secondary organic aerosol from biogenic amines 显示文摘 | Facchini M C Decesari S Rinaldi M Carbone C Finessi E Mircea M Fuzzi S Moretti F Tagliavini E Ceburnis D O'Dowd C D | 2008 | Environ Sci Technol2008，42，24： | 1 |
| 6 | Mineral nutrient partitioning to fruits of deciduous trees 显示文摘 | Tagliavini M Zavalloni C Rombola A D | 2000 | Acta Horti2000，512，： | 1 |
| 7 | Dynamics of uptake of calcium, potassium and magnesium into apple fruit in a high density planting显示文摘 | Zavalloni C Marangoni B Tagliavini M 2001 | 2001 | Acta Hort2001，564，： | 1 |
| 8 | Iron deficiency and chlorosis in orchard and vineyard Ecosystems显示文摘 | Tagliavini M Rombola A D | 2001 | European Journal of Agronomy2001，15，2： | 1 |
| 9 | Amyloid in Alzheimer's disease and prion-related encephalopathies:studies with synthetic peptides显示文摘 | FORLONI G TAGLIAVINI F BUGIANI O | 1996 | Prog Neurobiol1996，49，4： | 1 |
| 10 | Generalized granuloma annulare associated with scabies显示文摘 | Piana S Pizzigoni S Tagliavini E | 2010 | Am J Dermatopathol2010，32，5： | 1 |
| 11 | Iron source affects iron reduction and re-greening of kiwifruit (Actinidia deliciosa) leaves 显示文摘 | Rombola A D Bruggemann W Tagliavini M | 2000 | Journal of Plant Nutrition2000，23，： | 1 |
| 12 | Neurotoxicity of a prion protein fragment 显示文摘 | Forloni G Angeretti N Tagliavini F | 1993 | Nature1993，362，： | 1 |
| 13 | Influence of root pruning and water stress on growth and physiological factors of potted apple, grape, peach and pear trees显示文摘 | Poni S Tagliavini M Neri D | 1992 | Sci Hortic1992，，52： | 1 |
| 14 | Creutzfeldt-Jakob disease:activated microglia detected in vivo by molecular imaging显示文摘 | Moresco RM Messa C Tagliavini F | 2004 | Neuropathol Appl Neurobiol2004，30，4： | 1 |
| 15 | Major nutritional issues in deciduous fruit orchards of northern Italy显示文摘 | Tagliavini M Marangoni B | 2002 | Horttechnology2002，12，1： | 1 |
| 16 | Timing of nitrogen uptake affects winter storage and spring remobilisation of nitrogen in nectarine (Prunus persica var. nectarina) trees显示文摘 | Massimo Tagliavini Peter Millard Maurizio Quartieri Bruno Marangoni | 1999 | Plant and Soil1999，，2： | 1 |
| 17 | pre-postdilution haemofitration显示文摘 | David s Tagliavini d CAMBI V | 1989 | Nephrol Dial Transplant1989，4，： | 1 |
| 18 | Timing of nitrogen uptake affects winter storage and spring remobilisation of nitrogen in nectarine (Prunus persica var. nectarina) trees显示文摘 | Massimo Tagliavini Peter Millard Maurizio Quartieri Bruno Marangoni | 1999 | Plant and Soil1999，，2： | 1 |
| 19 | Influence of root pruning and water stress on growth and physiological factors of potted apple, grape, peach and pear trees显示文摘 | Poni S Tagliavini M Neri D | 1992 | Hort Sci1992，52，3： | 1 |
| 20 | Neuronal loss in the basal nucleus of Meynert in a patient with olivopontocerebellar atrophy显示文摘 | F. Tagliavini G. Pilleri | 1985 | Acta Neuropathologica1985，，2： | 1 |
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