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| 1 | Evaluation of in vitro digestibility of Aspergillus oryzae fungal biomass grown on organic residue derived‑VFAs as a promising ruminant feed supplement显示文摘Background As demand for high quality animal feed continues to raise,it becomes increasingly important to mini-mize the environmental impact of feed production.An appealing sustainable approach to provide feed fractions is to use organic residues from agro-food industry.In this regard,volatile fatty acids(VFAs)such as acetic,propionic and butyric acids,derived from bioconversion of organic residues can be used as precursors for production of micro-bial protein with ruminant feed inclusion potential.This study aims to investigate the in vitro digestibility of the Asper-gillus oryzae edible fungal biomass cultivated on VFAs-derived from anaerobic digestion of residues.The produced fungal protein biomass,along with hay clover silage and rapeseed meal were subjected to various in vitro assays using two-stage Tilley and Terry(TT),gas,and bag methods to evaluate and compare its digestibility for application in ruminant feed.Results The produced fungal biomass contained a higher crude protein(CP)(41%–49%)and rather similar neutral detergent fiber(NDF)(41%–56%)compared to rapeseed meal.The rumen in vitro dry matter digestibility(IVDMD)of the fungal biomass in the TT method ranged from 82%to 88%(statistically similar to that of the gas method(72%to 85%)).The IVDMD of fungal biomass were up to 26%and 40%greater than that of hay clover silage and rapeseed meal,respectively.The type of substrate and bag method had pronounced effect on the fermentation products(ammonium-N(NH4+-N),total gas and VFAs).Fungal biomass digestion resulted in the highest release of NH4+-N(340–540 mg/L)and the ratio of acetate to propionate ratio(3.5)among subjected substrates.Conclusion The results indicate that gas method can be used as a reliable predictor for IVDMD as well as fermenta-tion products.Furthermore,the high IVDMD and fermentation product observed for Aspergillus oryzae fungal biomass digestion,suggest that the supplementation of fungal biomass will contribute to improving the rumen digestion by providing necessary nitrogen and energy to the ruminant and microbiota. | Clarisse Uwineza Mohammadali Bouzarjomehr Milad Parchami Taner Sar Mohammad J.Taherzadeh Amir Mahboubi | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
| 2 | Conversion of fish processing wastewater into fish feed ingredients through submerged cultivation of Aspergillus oryzae显示文摘Fish processing towards production of fillet gives rise to wastewater streams that are ultimately directed to biogas production and/or wastewater treatment.However,these wastewater streams are rich in minerals,fat,and proteins that can be converted to protein-rich feed ingredients through submerged cultivation of edible filamentous fungi.In this study,the origin of wastewater stream,initial pH,cultivation time,and extent of washing during sieving,were found to influence the amount of recovered material from the wastewater streams and its protein content,following cultivation with Aspergillus oryzae.Through culti-vation of the filamentous fungus in sludge,330 kg of material per ton of COD were recovered by sieving,corresponding to 121 kg protein per ton of COD,while through its cultivation in salt brine,210 kg of material were recovered per ton of COD,corresponding to 128 kg protein per ton of COD.Removal ranges of 12-43%,39-92%,and 32-66%for COD,total solids,and nitrogen,respectively,were obtained after A.oryzae growth and harvesting in the wastewater streams.Therefore,the present study shows the versatility that the integration of fungal cultivation provides to fish processing industries,and should be complemented by economic,environmental,and feeding studies,in order to reveal the most promising valorization strategy. | Taner Sar Jorge A.Ferreira Mohammad J.Taherzadeh | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 0 |
| 3 | Demo-scale production of protein-rich fungal biomass from potato protein liquor for use as innovative food and feed products显示文摘Innovative food and feed products have recently attracted the attention of both producers and consumers.Filamentous fungi are important biomass producers with their high protein contents.In this study,fungal biomass production from edible potato protein liquor (PPL),generated during starch production processes,was investigated through different fungal strains (Rhizopus oryzae ,R.oligosporus ,R.delemar ,Aspergillus oryzae and Neurospora intermedia ).The effects of PPL concentration,incubation time,initial pH,and cultivation conditions (in shake flaks and different scale reactors) were examined to determine the amount of biomass and its crude protein level.It was determined that the fungal biomass produced by R.delemar in industrial scale contained 53% crude protein.For this strain,the amino acid and fatty acid profiles as well as metals (iron,manganese,copper,and zinc) of the produced biomass were also investigated to assess possible use as a food or feed source.The R.delemar fungal biomass can be a promising raw material for feed and food production,for example,considering its protein and fatty acid profiles with 41% essential amino acids and 33% polyunsaturated fatty acids. | Taner Sar Karin Larsson Rikard Fristedt Ingrid Undeland Mohammad J.Taherzadeh | 2022 | Food Bioscience2022，47，3： | 0 |
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