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| 1 | A novel artificial nerve graft for repairing longdistance sciatic nerve defects:a self-assembling peptide nanofiber scaffold-containing poly (lactic-co-glycolic acid) conduit显示文摘In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold(SAPNS)-containing poly(lactic-co-glycolic acid)(PLGA) conduit(SPC) and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury. | Xianghai Wang Mengjie Pan Jinkun Wen Yinjuan Tang Audra D.Hamilton Yuanyuan Li Changhui Qian Zhongying Liu Wutian Wu Jiasong Guo | 2014 | Neural Regeneration Research2014，9，24： | 5 |
| 2 | Characterization of Pythium chondricola associated with red rot disease of Pyropia yezoensis (Ueda)(Bangiales,Rhodophyta) from Lianyungang,China显示文摘Pyropia yezoensis(formerly Porphyra yezoensis)is an economically important red alga that is cultured extensively in China.The red rot disease occurs commonly during Pyropia cultivation,causing serious economic losses.An incidence of red rot disease was found in a P.yezoensis farm from mid-November to mid-December 2015 at Lianyungang,Jiangsu Province,China.Histopathological examination revealed that the naturally infected thalli were infected apparently by a pathogen,leading to red rot symptoms.The causative agent was isolated and identified as the oomycete Pythium chondricola by morphological analysis and sequence analysis of the internal transcribed spacer and cytochrome oxidase subunit 1(cox 1).In artifi cial infection experiments on the P.yezoensis blades,the P.chondricola isolate was able to cause the same characteristic histopathology seen in natural infections.P.chondricola grew well at a wide range of temperatures in the range 8-31℃,salinities at 0-45 and pH 5-9.In an orthogonal test used to determine the effects of environmental factors(temperature,salinity,and zoospore concentration)on infection,the data revealed that temperature was the most important factor to affect red rot disease development,with the optimal conditions for disease expansion being 20℃,35 salinity,and a zoospore concentration of 10^6 zoospores/mL.The results obtained from the present study prompted us to set up a comprehensive epidemiological study on Pyropia,which will provide support to maintain the healthy development of the Pyropia industry in China. | QIU Liping MAO Yunxiang TANG Lei TANG Xianghai MO Zhaolan | 2019 | Journal of Oceanology and Limnology2019，37，3： | 3 |
| 3 | Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease显示文摘To explore ef fective physiological indexes to distinguish the resistant lines and susceptible lines of Pyt hium porphyrae,the causal agent of red rot disease of Pyropia yezoensis,and establish the disease-resistance breeding strategy,we obtained and analyzed the candidate resistant and susceptible lines by population selection.Gametophytes of the candidate lines were cultured in seawater containing Pyt.porphyrae zoospores.Antioxidase activities,including superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO),were measured and compared between the two lines before and after infection.In the resistant lines,SOD and POD activities increased and then decreased,but PPO activity rose with the prolongation of the infection time.The phenylalanine ammonia lyase(PAL)activities also increased and then decreased after infection,but it had significantly different expression in the two lines without pathogen attack.The synthesis rates ofβ-1,3-glucanase,and cell-wall degrading enzyme were different from each other between the two lines after infection of P yt.porphyrae.Comparison in the contents of malondialdehyde(MDA)and reactive oxygen species(ROS)in the two lines showed that,the two contents varied synchronously in response to the pathogen attack.Changes of these enzymes activities or contents demonstrated that Pyr.yezoensis could resist against the pathogen of P yt.porphyrae with the antioxidant defense capacity.In addition,β-1.3-glucanase content showed extremely significant dif ference between the two lines,and the PAL had consistent expression dif ference.Therefore,phenylalanine ammonia lyase(PAL)andβ-1,3-glucanase can be considered as an ef fective index to distinguish susceptible line and resistant line,with which the workload of the resistant breeding could be reduced in the future. | ZOU Dandan TANG Xianghai QIU Liping MAO Yunxiang | 2020 | Journal of Oceanology and Limnology2020，38，2： | 1 |
| 4 | Rapid synthesis of high quality MCM-41 silica with ultrasound radiation显示文摘 | Xianghai Tang Sunwen Liu Yanqi Wang Weiping Huang Elena Sominski Oleg Palchik Yuri Koltypin Aharon Gedanken | 2000 | Chem Commun2000，21，： | 1 |
| 5 | High-efficiency separation and extraction of naphthenic acid from high acid oils using imidazolium carbonate ionic liquids显示文摘N-alkyl imidazolium carbonate ionic liquids were employed to separate and recover naphthenic acid from model oils.The effects of the cationic and anionic structures of ionic liquids and operating conditions on the deacidification performance were investigated.The deacidification performance of traditional organic solvents was also investigated for comparison.The results indicated that the naphthenic acid could be completely removed from the model oil with a small mass ratio of ionic liquid to oil.The extracted naphthenic acid was regenerated with a recovery of up to 92%.In addition,imidazolium carbonate ionic liquids could be successfully regenerated and recycled.The mechanism of interaction between imidazole ionic liquids and the naphthenic acid molecules were explained by Gauss calculation. | Fenghua Geng Rui Zhang Luo Wu Zheng Tang Han Liu Haiyan Liu Zhichang Liu Chunming Xu Xianghai Meng | 2022 | Chinese Journal of Chemical Engineering2022，35，1： | 1 |
| 6 | Rapid synthesis of high quality MCM -41 silica with ultrasound radiation 显示文摘 | Tang Xianghai Liu Suwen Wang Yanqin | 2000 | Chem Commun2000，，： | 1 |
| 7 | 显示文摘 | Xianghai Tang Suwen Liu Yanqin Wang | 2000 | Chem Commun2000，，21： | 1 |
| 8 | 显示文摘 | Wang Yanqin Tang Xianghai Yin Lunxiang | 2000 | Adv Mater2000，12，16： | 1 |
| 9 | Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in Pyropia yezoensis (Bangiales,Rhodophyta)显示文摘The genus Pyropia contains several important cultivated species.Genetic research in nori species has mainly focused on the cell nucleus,with few studies on organelles(chloroplast and mitochondria).Due to the high copy numbers of organelles in cells,which influence the development and traits of algae,it is necessary to study their genetic mechanism.In this study,the marine red alga Pyropia yezoensis,an important economic macroalga,was selected as the study object.To investigate organelle(chloroplast and mitochondria)inheritance in P.yezoensis,the wild type RZ(maternal strain)was crossed with the red mutant HT(paternal strain)and 30 color-sectors from 11 F1 gametophytic blades were examined.The complete chloroplast and mitochondrial genomes of the red mutant(HT)were assembled for the first time.One reliable and stable single nucleotide polymorphism(SNP)loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA,respectively,in subsequent experiments.PCR amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent,confirming that both chloroplast and mitochondrial genomes were inherited maternally in P.yezoensis.The inheritance pattern of organelles in P.yezoensis can be used to guide the hybridization and breeding of nori.Additionally,the organelle SNP markers developed in this study can be applied in subsequent genetic research. | Lu WANG Junhao WANG Yunke ZHU Zhengcai CUI Fanna KONG Xianghai TANG Yunxiang MAO | 2021 | Journal of Oceanology and Limnology2021，39，4： | 0 |
| 10 | Molecular Scrippsiella phylogenetic analysis of dinoflagellate trochoidea isolated from the East Asianwaters显示文摘以前的研究在 Scrippsiella trochoidea A. R 发现 intraspecific 差异。Loeblich III,广泛地分布式的石灰质的生产包囊的 dinoflagellate。在这研究, S 的三紧张(ST-1, ST-D6 和 ST-K ) 。从东方亚洲水孤立的 trochoidea 被学习,和另外的地理紧张,到解决他们的种系发生的关系。因为三个东方亚洲人孤立,原子编码 ribosomal DNA (rDNA ) 的二个高度多样的区域, 5.8S rDNA 和它的 flanking 内部抄录分隔符 1 和 2,并且大子单元的 rDNA 的 5 鈥 ? 部分(包含鈥淒1 鈥 ? 和鈥淒2 鈥 ? 领域) 被定序。从其它的相应序列地理孤立从 GenBank 数据库被选择,种系发生的关系从这些紧张的分子的数据被推断。S 的紧张。trochoidea 被发现聚类进三主要 clades (STR1, STR2 和 STR3 ) ,在以前的研究报导了。三中的二个拉紧 ST-1 和 ST-K,在 clade STR2 被组织,另外的紧张, ST-D6,属于 clade STR3。S 的 intraspecific 差异。东方亚洲水里的 trochoidea 使在一个区域的生态生理学上与生物地理学,人口动力学,和生命周期的数据在联合执行种系发生的调查必要。关键词 Scrippsiella trochoidea - 发展史 - rDNA - 分子的 systematics 由中国科学院的知识革新节目支持了(没有。KZCX2-YW-208 ) 并且中国(号码 40676072 ) | TANG Xianghai YURencheng ZHANG Qingchun WANG Yunfeng YAN Tian ZHOU Mingjiang | 2010 | Chinese Journal of Oceanology and Limnology2010，28，2： | 0 |
| 11 | Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis显示文摘The phosphatidylinositol(PI)signaling system,a central regulator of eukaryotic metabolism,is widely found in eukaryotes for regulating a variety of cell activities.Most of the genes in the PI signaling system were found conserved in Pyropia yezoensis.In this experiment,wortmannin was used as an inhibitor to inhibit the activity of phosphatidylinositol-3 kinase(PI3K),an important regulator of the PI signaling system.After wortmannin treatment,the mitotic division of P.yezoensis was signifi cantly inhibited in a dose-dependent manner,and the mitotic division percentage was reduced by 68.1%and 91.9%in the 5-and 10-μmol/L groups,respectively.When thalli were treated with wortmannin,the levels of reactive oxygen species(ROS)were signifi cantly decreased.Furthermore,the expression level of PI3K was inhibited and the expression levels of downstream genes regulated by PI3K was signifi cantly changed.In the PI3K-AGC signaling pathway,the expression levels of Serine/threonine protein kinase(AGC)and cyclindependent kinases A(CDKA)were downregulated,while WEE1 kinase gene(WEE1)was upregulated.Three nicotinamide adenine dinucleotide phosphate(NADPH)oxidase genes were downregulated after wortmannin treatment.These results indicate that the PI signaling system plays an important role in the regulation of cell activity in P.yezoensis.It was speculated that the growth and development of P.yezoensis might be regulated by P.yezoensis PI3K,which promoted the expression of the AGC gene and further regulates the expression of downstream WEE1 and CDKA genes to advance mitotic division,and also promoted the expression level of NADPH oxidase that regulates ROS homeostasis. | Yunke ZHU Xinran WANG Bin SUN Xianghai TANG Yunxiang MAO | 2022 | Journal of Oceanology and Limnology2022，40，3： | 0 |
| 12 | On microbial community of Pyropia haitanensis by metagenomic analysis显示文摘Microorganisms plays an important role in the growth of Pyropia haitanensis.To understand the structural and functional diversity of the microorganism community of P.haitanensis(PH40),the associated metabolic pathway network in cluster of orthologous groups(COG)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and carbohydrate-active enzymes(CAZymes)were explored in metagenomic analysis.DNA extraction from gametophytes of P.haitanensis was performed first,followed by library construction,sequencing,preprocessing of sequencing data,taxonomy assignment,gene prediction,and functional annotation.The results show that the predominant microorganisms of P.haitanensis were bacteria(98.98%),and the phylum with the highest abundance was Proteobacteria(54.64%),followed by Bacteroidetes(37.92%).Erythrobacter(3.98%)and Hyunsoonleella jejuensis(1.56%)were the genera and species with the highest abundance of bacteria,respectively.The COG annotation demonstrated that genes associated with microbial metabolism was the predominant category.The results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial community.Plant growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units(modules)were the other important pathways.The CAZyme annotation revealed that the starch might be an important carbon source for microorganisms.Glycosyl transferase family 2(GT2)and glycosyl transferase family 3(GT3)were the highly abundant families in glucoside transferase superfamily.Six metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin(vitamin B 12)and indole-3-acetic acid were obtained by binning method.They were confirmed to belong to Rhodobacterales and Rhizobiales,respectively.Our findings provide comprehensive insights into the microorganism community of Pyropia. | Junhao WANG Yunxiang MAO Guoying DU Xiaojiao LI Xianghai TANG | 2021 | Journal of Oceanology and Limnology2021，39，3： | 0 |
| 13 | Construction of high-density genetic linkage map of Pyropia yezoensis(Bangiales,Rhodophyta)and identifi cation of red color trait QTLs in the thalli显示文摘Pyropia yezoensis is an important macroalga because of its extensive global distribution and economic importance.Color is an important trait in the thalli of P.yezoensis,it is also an effective marker to identify the hybridization in genetic breeding.In this study,a high-density genetic linkage map was constructed based on high-throughput single nucleotide polymorphism(SNP)markers,and used for analyzing the quantitative trait loci(QTLs)of red color trait in the thalli of P.yezoensis.The conchospore undergoes meiosis to develop into an ordered tetrad,and each cell has a haploid phenotype and can grow into a single individual.Based on this theory,F1 haploid population was used as the mapping population.The map included 531 SNP markers,394.57 cM long on average distance of 0.74 cM.Collinear analysis of the genetic linkage map and the physical map indicated that the coverage between the two maps was 79.42%.Furthermore,QTL mapping identified six QTLs for the chromosomal regions associated with the red color trait of the thalli.The value of phenotypic variance explained(PVE)by an individual QTL ranged from 4.71%-63.11%.And QTL qRed-1-1,with a PVE of 63.11%,was considered the major QTL.Thus,these data may provide a platform for gene and QTL fine mapping,and marker-assisted breeding in P.yezoensis in the future. | Lu WANG Kuipeng XU Xianghai TANG Junhao WANG Fanna KONG Yunxiang MAO | 2021 | Journal of Oceanology and Limnology2021，39，3： | 0 |
| 14 | Estimating areal carbon fixation of intertidal macroalgal community based on composition dynamics and laboratory measurements显示文摘The community dynamics and potential carbon ?xation of intertidal macroalgae were investigated monthly from April 2014 to April 2015 in the northwest coast of Yellow Sea. Seasonal variations in biomass and carbon ?xation were presented and showed close relationship with community structure.The carbon ?xation rate ranged from 0.48±0.13 mg C/(g FW ·d) to 4.35±0.12 mg C/(g FW ·d). Sargassum thunbergii, Chondrus ocellatus and Ulva intestinalis were three most in?uential species which contributed27%, 21.9% and 18.5% variation of carbon ?xation rate, respectively. Standing carbon stocks ranged from7.52 g C/m^2 to 41.31 g C/m^2, and estimated carbon stocks varied from 11.77 g C/m 2 to 96.49 g C/m^2. The larger dif ference between estimated and standing carbon stocks implied that more ?xed carbon was exported from the community in summer and autumn than in winter. This study suggested that intertidal macroalgal community could provide a potential function in carbon ?xation of coastal ecosystem. | DU Guoying ZHAO Ezi LIU Chunrong TANG Xianghai | 2019 | Journal of Oceanology and Limnology2019，37，1： | 0 |
| 15 | Comparison in structure and predicted function of epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae显示文摘The economic alga Neopyropia,which holds significant value in China for its edibility and economic potential,harbors diverse epiphytic bacteria on its surface.The epiphytic bacteria on Neopyropia yezoensis and Neopyropia katadae,two nori species of Neopyropia living at the same tidal level but with distinct physiological states and living habits,were investigated to understand the differences between them and the effects of epiphytic bacteria on their differential characteristics.Analysis of 16S rRNA gene sequences and real-time quantitative PCR(qPCR)of thalli and seawater samples(n=24)revealed notable differences in microbial diversity and community structure between the two species.Bacteroidetes dominated the bacterial communities in association with N.yezoensis,whereas Proteobacteria were predominant in N.katadae.Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt2)analysis revealed enriched genes that related to the ABC transport system,iron complex outer membrane receptor proteins,and proliferation in N.yezoensis,whereas N.katadae exhibited enrichment of genes that related to energy supply as well as cell wall and cell membrane production.The results of qPCR indicate a higher abundance of epiphytic bacteria on surface of N.yezoensis than those on surface of N.katadae.The findings indicate that differences in the living environments of N.yezoensis and N.katadae may result in distinct structural composition and abundance of epiphytic bacteria on their surfaces,thereby conferring specific biological functionalities to each microbial community,and the epiphytic bacteria may shape the living habits of hosts to some extents.This study provided a basis for understanding the close association between surface microorganisms and algal bodies,and the physiological and ecological characteristics of nori species. | Xinran WANG Junhao WANG Yunke ZHU Xinyu ZHU Hong QIN Ka BIAN Xianghai TANG | 2023 | Journal of Oceanology and Limnology2023，41，6： | 0 |
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