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| 1 | Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy显示文摘Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue section.This review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell interactions.Among these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly promising.mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue studies.This technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy. | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2020 | Cancer Communications2020，40，4： | 11 |
| 2 | Benign prostatic hyperplasia and male lower urinary symptoms:A guide for family physicians显示文摘Male patients with lower urinary tract symptoms(LUTS)and benign prostatic hyperplasia(BPH)are increasingly seen by family physicians worldwide due to ageing demographics.A systematic way to stratify patients who can be managed in the community and those who need to be referred to the urologist is thus very useful.Good history taking,physical examination,targeted blood or urine tests,and knowing the red flags for referral are the mainstay of stratifying these patients.Case selection is always key in clinical practice and in the setting of the family physician.The best patient to manage is one above 40 years of age,symptomatic with nocturia,slower stream and sensation of incomplete voiding,has a normal prostatespecific antigen level,no palpable bladder,and no haematuria or pyuria on the labstix.The roles of α blockers,5-α reductase inhibitors,and antibiotics in a primary care setting to manage this condition are also discussed. | Farhad Fakhrudin Vasanwala Michael Yuet Chen Wong Henry Sun Sien Ho Keong Tatt Foo | 2017 | Asian Journal of Urology2017，4，3： | 5 |
| 3 | Can intravesical prostatic protrusion predict bladder outlet obstruction even in men with good flow?显示文摘Objective:Men with benign prostate hyperplasia(BPH)with good urinary flow may still have bladder outlet obstruction(BOO).Intravesical prostatic protrusion(IPP)has been shown to be able to predict BOO.We aim to investigate the use of IPP to predict BOO in men with good urinary flow.Methods:One hundred and fourteen consecutive men(>50 years old)presenting with lower urinary tract symptoms suggestive of BPH were recruited in 2001 and 2002.They were evaluated with serum prostate specific antigen(PSA),uroflowmetry and transabdominal ultrasound measurement of IPP and prostate volume(PV).Pressure-flow urodynamic studies were performed on all men and BOO was defined by BOO index>40.Men with Qmax12.0 mL/s were considered to have good flow.Results:Among the 114 men,61 patients had good urinary flow.Their median age,PV and Qmax were 66 years,32.9 mm3 and 14.5 mL/s respectively.14/61(23.0%)patients had BOO and their distribution of IPP were as follows:Grade 1 e 0/20(0%)obstructed,Grade 2 e 6/22(27.3%)and Grade 3 e 8/19(42.1%).Sensitivity of Grade 2/3 IPP for BOO was 100% while specificity of Grade 3 IPP was 76.6%.The area-under-curve(AUC)for IPP was greater than that for PV(0.757 vs.0.696).Conclusion:Even in men with good flow,high grades of IPP were more likely to have BOO and hence,may be a useful adjunct to predict BOO. | Alvin Lee Han Jie Lee Kok Bin Lim Hong Hong Huang Henry Ho Keong Tatt Foo | 2016 | Asian Journal of Urology2016，3，1： | 4 |
| 4 | Cytotoxic CD8+ T cells and tissue resident memory cells in colorectal cancer based on microsatellite instability and BRAF status显示文摘BACKGROUND Recent studies in non-colorectal malignancy have associated T resident memory(T_(RM)) cells with improved patient survival. It is unknown if T_(RM) plays a role in colorectal cancer(CRC).AIM To examine the potential role of T_(RM) cells in providing immunogenicity in CRC stratified by microsatellite instability(MSI) and BRAF status.METHODS Patients with known MSI and BRAF mutation status were eligible for inclusion in this study. CRC tumour sections stained with haematoxylin and eosin were microscopically reviewed and the images scanned prior to assessment for location of invading edge and core of tumour. Sequential sections were prepared for quantitative multiplex immunohistochemistry(IHC) staining. Opal Multiplex IHC staining was performed with appropriate positive and negative controls and imaged using a standard fluorescent microscope fitted with a spectral scanning camera(Mantra) in conjunction with Mantra snap software. Images were unmixed and annotated in in Form 2.2.0. Statistical analysis was performed using Graphpad Prism Version 7 and Stata Version 15.RESULTS Seventy-two patients with known MSI and BRAF status were included in the study. All patients were assessed for MSI by IHC and high resolution capillary electrophoresis testing and 44 of these patients successfully underwent quantitative multiplex IHC staining. Overall, there was a statistically significant increase in CD8+ T_(RM) cells in the MSI(BRAF mutant and wild type) group over the microsatellite stable(MSS) group. There was a statistically significant difference in CD8+ T_(RM) between high level MSI(MSI-H):BRAF mutant [22.57, 95% confidence interval(CI): 14.31-30.84] vs MSS [8.031(95%CI: 4.698-11.36)], P = 0.0076 and MSI-H:BRAF wild type [16.18(95%CI: 10.44-21.93)] vs MSS [8.031(95%CI: 4.698-11.36)], P = 0.0279. There was no statistically significant difference in CD8 T cells(both CD8+CD103-and CD8+CD103+T_(RM)) between MSI-H: BRAF mutant and wild type CRC.CONCLUSION This study has shown that CD8+ T_(RM) are found in greater abundance in MSI-H CRC, both BRAF mutant and MSI-H:BRAF wild type, when compared with their MSS counterpart. CD8+ T_(RM) may play a role in the immunogenicity in MSI-H CRC(BRAF mutant and BRAF wild type). Further studies should focus on the potential immunogenic qualities of T_(RM) cells and investigate potential immunotherapeutic approaches to improve treatment and survival associated with CRC. | James Wei Tatt Toh Angela L Ferguson Kevin J Spring Hema Mahajan Umaimainthan Palendira | 2021 | World Journal of Clinical Oncology2021，12，4： | 2 |
| 5 | 镍和钒在裂化催化剂上的相互作用显示文摘 | Tatt,BF 蔡庆琪 | 1990 | 石油炼制译丛1990，，4： | 2 |
| 6 | Solving the benign prostatic hyperplasia puzzle显示文摘1.Introduction Benign prostatic hyperplasia(BPH)is a common urological condition,and yet there are many puzzles waiting to be solved.This perspective is to help explain some of these puzzles,based on our recent publications and previous clinical studies on BPH.2.Why small prostates obstruct and some big prostates do not obstruct? | Keong Tatt Foo | 2016 | Asian Journal of Urology2016，3，1： | 2 |
| 7 | Patients with small prostates and low-grade intravesical prostatic protrusion e A urodynamic evaluation显示文摘Abstract Objective:Despite high-grade intravesical prostatic protrusion(IPP)being closely related to bladder outlet obstruction(BOO),up to 21%of patients with low IPP remain obstructed.This study evaluates the characteristics and urodynamic findings of men with small prostates and low IPP.Methods:One hundred and fourteen men aged>50 years old with lower urinary tract symptoms(LUTS)were assessed with symptoms,uroflowmetry,serum prostate-specific antigen(PSA),transabdominal ultrasound measurement of prostate volume(PV),IPP and post-void residual urine(PVRU).All patients underwent pressure flow studies.Patients with PV<30 mL and IPP10 mm were examined for parameters correlating with BOO or impaired detrusor contractility.Results:Thirty-six patients had PV<30 mL and IPP<10 mm.Nine patients(25.0%)had urodynamic BOO,all with normal bladder contractility.Fourteen patients(38.9%)had poor detrusor contractility and all had no BOO.PV,PVRU and IPP were significantly associated with BOO,with IPP showing greatest positive correlation.Both Qmax and IPP were significantly associated with detrusor contractility.At 5-year follow-up,most patients responded to medical therapy.Only three out of nine patients(33.3%)with BOO eventually underwent surgery,and all had a high bladder neck seen on the resectoscope.Only one patient(7.1%)with poor detrusor contractility eventually required surgery after repeat pressure flow study revealed BOO.Conclusion:In men with small prostates and low IPP,the presence of BOO is associated with higher PV,PVRU and IPP,and most respond well to medical management.BOO can possibly be explained by elevation of the bladder neck by a small subcervical adenoma. | Han Jie Lee Alvin Lee Hong Hong Huang Palaniappan Sundaram Keong Tatt Foo | 2017 | Asian Journal of Urology2017，4，4： | 2 |
| 8 | 肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术显示文摘常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术。但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物。本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合研究。在这些技术中,多重免疫组织化学/免疫荧光(multiplex immunohistochemistry/immunofluorescence,mIHC/IF)已成为最具应用前景的技术。mIHC/IF为开展高重复性、高效、高性价比的组织研究提供了高通量多重染色技术和标准化的定量分析。该技术在转化研究和临床实践中,尤其是肿瘤免疫治疗中极具应用价值。 | Wei Chang Colin Tan Sanjna Nilesh Nerurkar Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim | 2021 | 癌症2021，40，9： | 2 |
| 9 | Pathophysiology of clinical benign prostatic hyperplasia显示文摘A disease can be defined as an abnormal anatomy(pathology)and/or function(physiology)that may cause harm to the body.In clinical benign prostatic hyperplasis(BPH),the abnormal anatomy is prostate adenoma/adenomata,resulting in a varying degree of benign prostatic obstruction(BPO)that may cause harm to the bladder or kidneys.Thus clinical BPH can be defined as such and be differentiated from other less common causes of male lower urinary tract symptoms.Diagnosis of the prostate adenoma/adenomata(PA)can be made by measuring the intravesical prostatic protrusion(IPP)and prostate volume(PV)with non-invasive transabdominal ultrasound(TAUS)in the clinic.The PA can then be graded(phenotyped)according to IPP and PV.Multiple studies have shown a good correlation between IPP/PV and BPO,and therefore progression of the disease.The severity of the disease clinical BPH can be classified into stages from stage Ⅰ to Ⅳ for further management.The classification is based on the effect of BPO on bladder functions,namely that of emptying,normal if postvoid residual urine(PVRU)<100 mL;and bladder storage,normal if maximum voided volume(MVV)>100 mL.The effect of BPO on quality of life(QoL)can be assessed by the QoL index,with a score≥3 considered bothersome.Patients with no significant obstruction and no bothersome symptoms would be stage Ⅰ;those with no significant obstruction but has bothersome symptoms(QoL≥3)would be stage Ⅱ;those with significant obstruction(PVRU>100 mL;or MVV<100 mL),irrespective of symptoms would be stage Ⅲ;those with complications of the disease clinical BPH such as retention of urine,bladder stones,recurrent bleeding or infections would be stage Ⅳ.After assessment,further management can then be individualised.A low grade and stage disease can generally be watched(active surveillance)while a high grade and stage disease would need more invasive management with an option for surgery.The final decision making would take into account the patient’s age,co-morbidity,social economic background and his preferences/values. Proper understanding of pathophysiology of clinical BPH would lead to better selection of patients for individualised and personalised care andmore cost effective management. | Keong Tatt Foo | 2017 | Asian Journal of Urology2017，4，3： | 2 |
| 10 | Tools for assessing quality and susceptibility to bias in observational studies in epidemiology:a systematic review and annotated bibliography显示文摘 | Sanderson S Tatt ID Higgins JPT | 2007 | Int J Epidemiol2007，36，3： | 1 |
| 11 | Tools for assessing quality and susceptibility to bias in observational studies in epidemiology;a sys- tematic review and annotated bibliography显示文摘 | Sanderson S Tatt ID Higgins JPT | 2007 | Int J Epidemiol2007，36，3： | 1 |
| 12 | Tools for assessing quality and susceptibility to bias in observational studies in epidemiology: a systematic review and annotated bibliography显示文摘 | Sanderson S Tatt ID Higgins JP | 2007 | International Journal of Epidemiology2007，36，： | 1 |
| 13 | Tools for assessing quality and susceptibillity to bias in observational studies in epidmiology: a systematic review and annotated bibliography 显示文摘 | SANDERSON S TATT ID HIGGINS JP | 2007 | Int J Epidemiol2007，36，3： | 1 |
| 14 | Flowinjection determination of peroxide value in edible oils usingtriiodide detector显示文摘 | Bahruddin Saad Wan Tatt Wai Boey Peng Lim et a1 | 2006 | Analytical Chimica Acta2006，565，： | 1 |
| 15 | An unusual case of right-sided colon cancer with isolated lateral pelvic side wall lymph node metastases显示文摘Lymphatic drainage is typically in the direction of arterial supply,and lymphatic drainage for right-sided colon cancers typically travels to ileocolic lymph nodes.It is rare for right-sided colon cancers to metastasize to the lateral pelvic side wall lymph nodes in the absence of local invasion or other distant metastases.In this report,we present an unusual case of a young female with pT4a right-sided ascending colon cancer with isolated metastases to the pelvic iliac lymph nodes.The patient underwent minimally invasive laparoscopic right hemicolectomy and pelvic lymph node dissection with curative intent.She recovered well with no intraoperative complications and was referred for urgent adjuvant chemotherapy and radiotherapy. | Rishaan Pawaskar James Wei Tatt Toh | 2022 | Laparoscopic, Endoscopic and Robotic Surgery2022，5，3： | 1 |
| 16 | Seven lipoprotein lipase gene polymorphisms, lipid fractions, and coronary disease: a HuGE asso- ciation review and meta-analysis显示文摘 | Sagoo G S Tatt I Salanti G | 2008 | American journal of epidemiol- ogy2008，168，11： | 1 |
| 17 | Sequence and phylogenetic analysis of the VP6 and NSP4 genes of human rotavirus strains:evidence of discordance in their genetic linkage显示文摘 | Tatte V S Rawal K N Chitambar S D | 2010 | Infection Genetics and Evolution2010，10，7： | 1 |
| 18 | Flow injection determination of peroxide value in edible oils using triiodide detector 显示文摘 | Bahruddin Saad Wan Tatt Wai Boey Peng Lim | 2006 | Analytica Chimica Acta2006，565，： | 1 |
| 19 | Hypomagnesaemia associated with long-term use of proton pump inhibitors显示文摘Hypomagnesaemia and associated hypocalcaemia and hypoparathyroidism have been increasingly recognised as rare longterm side-effects of proton pump inhibitors(PPIs).The PPIs may inhibit active magnesium(Mg)absorption by interfering with transcellular transient receptor potential melastatin-6 and-7(TRPM 6 and 7)channels.More recent cell culture studies have suggested concomitant inhibition of passive Mg absorption by omeprazole.After being treated with a range of PPIs,the four patients in our case series developed hypomagnesaemia,which responded to withdrawal of therapy and initiation of Mg replacement.Their clinical course and management demonstrate key aspects of hypomagnesaemia associated with long-term use of PPIs. | James Wei Tatt Toh Evonne Ong Robert Wilson | 2015 | Gastroenterology Report2015，3，3： | 1 |
| 20 | Seven lipoprotein lipase gene polymorphisms,lipid fractions,and coronary disease:a Hu GE association review and meta-analysis显示文摘 | Sagoo GS Tatt I Salanti G | 2008 | Am J Epidemiol2008，168，11： | 1 |
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