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2篇 您的检索式：作者名="Tiansheng Lu"

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		题名	作者	年代	出处	被引量
	1	Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning显示文摘Previous studies have demonstrated that pine pollen can inhibit cerebral cortical cell apoptosis in mice with arsenic poisoning.The present study sought to detect the influence of pine pollen on apoptosis-related proteins.Immunohistochemistry,western blotting and enzyme-linked immunosorbent assays were used to measure the levels of apoptosis-related proteins in the cerebral cortex of mice with arsenic poisoning.Results indicated that pine pollen suppressed cell apoptosis in the cerebral cortex of arsenic-poisoned mice by reducing Bax,Bcl-2 protein expression and increasing p53 protein expression.	Yanhong Luo Yaodong Wei Taizhong Wang Dongzhu Chen Tiansheng Lu Ruibo Wu Keke Si	2012	Neural Regeneration Research2012，7，12：	4
	2	Efficient gene editing in a medaka(Oryzias latipes)cell line and embryos by SpCas9/tRNA-gRNA显示文摘Generation of mutants with clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)is commonly carried out in fish species by co-injecting a mixture of Cas9 messenger RNA(mRNA)or protein and transcribed guide RNA(gRNA).However,the appropriate expression system to produce functional gRNAs in fish embryos and cells is rarely present.In this study,we employed a poly-transfer RNA(tRNA)-gRNA(PTG)system driven by cytomegalovirus(CMV)promoter to target the medaka(Oryzias latipes)endogenous gene tyrosinase(tyr)or paired box 6.1(pax6.1)and illustrated its function in a medaka cell line and embryos.The PTG system was combined with the CRISPR/Cas9 system under high levels of promoter to successfully induce gene editing in medaka.This is a valuable step forward in potential application of the CRISPR/Cas9 system in medaka and other teleosts.	Qihua PAN Junzhi LUO Yuewen JIANG Zhi WANG Ke LU Tiansheng CHEN	2022	Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，1：	0

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